BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_O15
(911 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.011
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.17
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.69
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 29 0.69
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.8
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.5 bits (78), Expect = 0.011
Identities = 37/150 (24%), Positives = 41/150 (27%), Gaps = 8/150 (5%)
Frame = +3
Query: 450 PPAAXGGXXGXLXXLPPPXXXXXXX-----PXGRXGXPPXXXXGGXPRXPXXPXETPXXX 614
PP G L PPP P GR PP PR P
Sbjct: 325 PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP-----PRSAPSTGRQPPPL 379
Query: 615 XXPKGXXXPKXPEXPAXXGFXXXXXXXLKNXXXKTXXXRXRPXRKGXXXIPXVTPXXPPX 794
+ P P PA G L N + P P + P PP
Sbjct: 380 SSSRAVSNPPAPP-PAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPS 438
Query: 795 ---GPPPXRPXXXRXXLPPPXPRGETGAPP 875
G P P + PP P G APP
Sbjct: 439 LPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
Score = 28.7 bits (61), Expect = 1.2
Identities = 20/80 (25%), Positives = 23/80 (28%), Gaps = 9/80 (11%)
Frame = +1
Query: 592 RGKXPXXXXGQRGXXPPXPXXGRRXL---------GXXXPPPPPXKTTXXKPXXXXPGQX 744
RGK P PP P R G PPPPP ++ P
Sbjct: 322 RGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSS 381
Query: 745 XKXXKXSPG*PPXXPPXXPP 804
+ P PP P P
Sbjct: 382 SRAVSNPPAPPPAIPGRSAP 401
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.5 bits (68), Expect = 0.17
Identities = 31/137 (22%), Positives = 36/137 (26%), Gaps = 9/137 (6%)
Frame = +3
Query: 492 LPPPXXXXXXXPXGRXGXPPXXXXGGXPRXPXXPXETPXXXXXPKGXXXPKX----PEXP 659
+P P P PP G P P P PK P P
Sbjct: 1094 VPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVP 1153
Query: 660 AXXGFXXXXXXXLKNXXXKTXXXRXRPXRKGXXXIPXVTPXXPPXGPPPXRPXXXRXXLP 839
A G + P K +P V P P PP +P +P
Sbjct: 1154 APSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP--PSEAPPVPKPSVGVPPVP 1211
Query: 840 PPX-----PRGETGAPP 875
PP P G PP
Sbjct: 1212 PPSTAPPVPTPSAGLPP 1228
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/39 (33%), Positives = 14/39 (35%)
Frame = +1
Query: 688 PPPXKTTXXKPXXXXPGQXXKXXKXSPG*PPXXPPXXPP 804
PP K P P + K S G PP PP P
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAP 1217
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/41 (31%), Positives = 17/41 (41%), Gaps = 2/41 (4%)
Frame = +3
Query: 762 IPXVTPXXPPXGPPPXRPXXXRXXLPPPXPRGETGAP--PP 878
+P + PP P P PP P+ +GAP PP
Sbjct: 1024 VPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPP 1064
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.5 bits (63), Expect = 0.69
Identities = 15/35 (42%), Positives = 15/35 (42%)
Frame = +3
Query: 774 TPXXPPXGPPPXRPXXXRXXLPPPXPRGETGAPPP 878
TP P PP P PPP P G GA PP
Sbjct: 743 TPAPAPIPVPPPAPIMGGPP-PPPPPPGVAGAGPP 776
Score = 28.3 bits (60), Expect = 1.6
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +1
Query: 628 GXXPPXPXXGRRXLGXXXPPPPP 696
G PP P G G PPPPP
Sbjct: 760 GPPPPPPPPGVAGAGPPPPPPPP 782
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 29.5 bits (63), Expect = 0.69
Identities = 25/70 (35%), Positives = 26/70 (37%), Gaps = 6/70 (8%)
Frame = -1
Query: 809 ARGGXXGGXXGGYPG-----DXXXXFXXWP-GSXXXGFXXVVFXGGGGGXXKPXXRRPFX 648
A GG GG GG G D F G G +F GGG G P RR
Sbjct: 134 AGGGGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAG---PHARRSHP 190
Query: 647 GFGGXXPLWP 618
FGG P P
Sbjct: 191 SFGGSRPSQP 200
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/48 (33%), Positives = 17/48 (35%)
Frame = +3
Query: 735 RPXRKGXXXIPXVTPXXPPXGPPPXRPXXXRXXLPPPXPRGETGAPPP 878
RP + TP PP PP PP P G APPP
Sbjct: 1693 RPQSAAPPQMSAPTPPPPPMSVPPPPSA-------PPMPAGPPSAPPP 1733
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.149 0.524
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,612,575
Number of Sequences: 5004
Number of extensions: 19352
Number of successful extensions: 89
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 68
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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