BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_O06
(989 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 35 0.015
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.027
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.58
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 28 1.8
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 3.1
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 5.4
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 5.4
SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|... 27 5.4
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 35.1 bits (77), Expect = 0.015
Identities = 35/123 (28%), Positives = 37/123 (30%)
Frame = +3
Query: 573 PXXLXXPQXXXPXXKXPXXGPPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXSGX 752
P + P P P PP PA P PP P P P SG
Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPP-VPAPSGIPPVPKPSVAAPPV-PKPSVAVPPVPAPSGA 1120
Query: 753 RAXXXXPXXXKPXXXTXPXXVRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPPHPXXGXXG 932
P KP P V PSG P P PP P P G PP P
Sbjct: 1121 ------PPVPKPSVAAPPVPV--PSGAPPVPK--PSVAAPPVPA--PSGAPPVPKPSVAA 1168
Query: 933 PNI 941
P +
Sbjct: 1169 PPV 1171
Score = 34.3 bits (75), Expect = 0.027
Identities = 33/132 (25%), Positives = 35/132 (26%), Gaps = 10/132 (7%)
Frame = +3
Query: 591 PQXXXPXXKXPXXGPPRXPAYRSXEXPPXXXX---KXPPXXPPPXPXXTXXPXXSGXR-- 755
P P P PP P S P PP P P P SG
Sbjct: 1027 PSADAPPIPVPSTAPP-VPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPV 1085
Query: 756 -AXXXXPXXXKPXXXTXPXX----VRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPPHPXX 920
A P KP P P P AP P P +P G PP P
Sbjct: 1086 PAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKP 1145
Query: 921 GXXGPNITLXRG 956
P + G
Sbjct: 1146 SVAAPPVPAPSG 1157
Score = 28.3 bits (60), Expect = 1.8
Identities = 26/117 (22%), Positives = 28/117 (23%)
Frame = +3
Query: 591 PQXXXPXXKXPXXGPPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXSGXRAXXXX 770
P P P PP S PP P P P P S
Sbjct: 1126 PSVAAPPVPVPSGAPPVPKP--SVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPK 1183
Query: 771 PXXXKPXXXTXPXXVRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPPHPXXGXXGPNI 941
P P P + P P PP P G PP P P +
Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTP-SAGLPPVPVPTAKAPPV 1239
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.3 bits (75), Expect = 0.027
Identities = 23/96 (23%), Positives = 23/96 (23%)
Frame = +3
Query: 621 PXXGPPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXSGXRAXXXXPXXXKPXXXT 800
P PPR A S PP PP P P P P P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 801 XPXXVRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPP 908
P G P P P P PP
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433
Score = 30.7 bits (66), Expect = 0.33
Identities = 27/106 (25%), Positives = 30/106 (28%), Gaps = 1/106 (0%)
Frame = +3
Query: 633 PPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXSGXRAXXXXPXXXKP-XXXTXPX 809
PP P+ R E P PP PPP P S R P P T
Sbjct: 233 PPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPR--PIAPVSMNPAINSTSKP 290
Query: 810 XVRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPPHPXXGXXGPNITL 947
+ PS A PP P P G N +L
Sbjct: 291 PLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSL 336
Score = 30.3 bits (65), Expect = 0.44
Identities = 19/54 (35%), Positives = 19/54 (35%)
Frame = +2
Query: 770 PPXXKTPXXNXAXXXPXPLRDPQXXXXPXXXRPPPPPXXNPPXXXPXPPXGXXR 931
PP P N A P P PQ P PPPPP P PP R
Sbjct: 337 PPPPPPPRSNAAGSIPLP---PQGRSAP----PPPPPRSAPSTGRQPPPLSSSR 383
Score = 30.3 bits (65), Expect = 0.44
Identities = 27/106 (25%), Positives = 30/106 (28%)
Frame = +3
Query: 591 PQXXXPXXKXPXXGPPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXSGXRAXXXX 770
P P P PP A R+ P PP PP P G A
Sbjct: 391 PPPAIPGRSAPAL-PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPA---- 445
Query: 771 PXXXKPXXXTXPXXVRXPSGTPKXAPXXPXXDXPPXPXXIPXGXPP 908
P + P P+G P P P PP P P P
Sbjct: 446 ---APPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP-PAPAPAPAAP 487
Score = 29.5 bits (63), Expect = 0.77
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 866 PPPPPXXNPPXXXPXPPXGXXRP 934
PPPPP N P PP G P
Sbjct: 339 PPPPPRSNAAGSIPLPPQGRSAP 361
Score = 26.2 bits (55), Expect = 7.1
Identities = 23/92 (25%), Positives = 26/92 (28%), Gaps = 9/92 (9%)
Frame = +3
Query: 633 PPRXPAYRSXEXPPXXXXKXPPXXPPPXPXXTXXPXXS------GXRAXXXXPXXXKPXX 794
PP P+ R+ PP PPP P S G A P P
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPST 372
Query: 795 XTXPXXV---RXPSGTPKXAPXXPXXDXPPXP 881
P + R S P P P P P
Sbjct: 373 GRQPPPLSSSRAVSNPPAPPPAIPGRSAPALP 404
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.9 bits (64), Expect = 0.58
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2
Query: 866 PPPPPXXNPPXXXPXPP 916
PPPPP PP P PP
Sbjct: 12 PPPPPGFEPPSQPPPPP 28
Score = 26.2 bits (55), Expect = 7.1
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 851 PXXXRPPPPPXXNPPXXXPXPP 916
P PPPPP P P PP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPP 27
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.3 bits (60), Expect = 1.8
Identities = 19/75 (25%), Positives = 20/75 (26%), Gaps = 1/75 (1%)
Frame = +3
Query: 693 PPXXP-PPXPXXTXXPXXSGXRAXXXXPXXXKPXXXTXPXXVRXPSGTPKXAPXXPXXDX 869
PP P PP P P S P PS P A
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKS 183
Query: 870 PPXPXXIPXGXPPHP 914
PP +P PP P
Sbjct: 184 PPSAPSLPSAVPPMP 198
Score = 25.8 bits (54), Expect = 9.4
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +2
Query: 866 PPPPPXXNPPXXXPXPP 916
PPPPP P P PP
Sbjct: 6 PPPPPAPAPAAAAPAPP 22
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.5 bits (58), Expect = 3.1
Identities = 20/104 (19%), Positives = 23/104 (22%)
Frame = +2
Query: 632 PPPXXXISIXRXPPXXXXQXTPXTXTXXPXXNQXPXXERXXXXXXXPPXXKTPXXNXAXX 811
PPP P P P + P P P
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHE 306
Query: 812 XPXPLRDPQXXXXPXXXRPPPPPXXNPPXXXPXPPXGXXRPQHH 943
+ P P PPPP P P PP + H
Sbjct: 307 PGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPFKHHELEEH 350
>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 557
Score = 26.6 bits (56), Expect = 5.4
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 4/32 (12%)
Frame = +2
Query: 206 DYNPNG-NGYEPIDNGAYYVDPPQG---RPYF 289
DYN N N Y PI N Y+++ G PYF
Sbjct: 119 DYNNNRKNFYPPIQNSTYFINATGGIDSMPYF 150
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = -2
Query: 913 GXGGXPWGIXXGXGGXSXXGW 851
G GG P G G GG GW
Sbjct: 253 GFGGGPGGFGGGPGGHGGPGW 273
>SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase
Pmp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 278
Score = 26.6 bits (56), Expect = 5.4
Identities = 14/33 (42%), Positives = 16/33 (48%)
Frame = +2
Query: 200 PKDYNPNGNGYEPIDNGAYYVDPPQGRPYFKPT 298
PK PN N +P NG + PP Y KPT
Sbjct: 46 PKASKPNSN--QPYPNGPVCIYPPNIYLYAKPT 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,493,884
Number of Sequences: 5004
Number of extensions: 40603
Number of successful extensions: 189
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 511279616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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