Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_M19
(887 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 153 4e-38
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 58 2e-09
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 58 2e-09
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 44 3e-05
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 37 0.004
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 36 0.010
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 28 2.0
SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomy... 26 8.2
>SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 194
Score = 153 bits (370), Expect = 4e-38
Identities = 74/160 (46%), Positives = 103/160 (64%), Gaps = 1/160 (0%)
Frame = +1
Query: 103 NEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFTRRNLAE 282
+++K ++ +M G++GDT FAEYIA NI LY +R+ L P A ASFTR+ LA
Sbjct: 31 DDDKSQILNSHNLMLYCGEAGDTTNFAEYIAANISLYTLRHNLNLSPEATASFTRKQLAT 90
Query: 283 YLRSSTPYFVNVLMGGYDKENG-PELYFMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKK 459
LRS PY VN+L+ GY+ G PEL+++DYLA+ V+VP+A GY + LSI DRY+K
Sbjct: 91 SLRSRKPYQVNILLAGYETNLGKPELFWLDYLATCVRVPYACQGYSSFYCLSIFDRYYKP 150
Query: 460 DATETEAYDILKKCVQEVHKRLFVSLPNFQVTVVNRDGIK 579
D T EA I+K C E+ KR+ + F VV++DGI+
Sbjct: 151 DLTIDEAVRIMKLCFDELKKRMPIDFKGFICKVVDKDGIR 190
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 58.0 bits (134), Expect = 2e-09
Identities = 41/163 (25%), Positives = 73/163 (44%), Gaps = 6/163 (3%)
Frame = +1
Query: 109 EKIYKISDRLVMGVIGDSGDTNQFAEYIAK-NIQLYKMRNGYELGPSAAASFTRRNLAEY 285
E++ K+ D ++G GD D Q + K I+ +GY L PS + + L
Sbjct: 75 ERLTKVGDNTIVGAGGDISDYQQIQRLLEKLEIKEGNYGDGYALQPSYIHEYLSKVLYAR 134
Query: 286 LRSSTPYFVNVLMGGYDKEN-GPELYFMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKD 462
PY+ +++ G D EN P + F D ++ P A G+ +L+L ++ + D
Sbjct: 135 RNKLDPYWNQLIVAGVDGENKEPYVAFADLRGTTYSAPAIATGFAMHLALPMLRKATDDD 194
Query: 463 ATETEAYDILKKCVQEVHKRLFV----SLPNFQVTVVNRDGIK 579
+T + + + + E + LF SL F V + +GI+
Sbjct: 195 RWKTLSKESARATIDECMRVLFYRDARSLNKFSVATITPEGIE 237
>SPAC23D3.07 |pup1||20S proteasome component beta
2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 57.6 bits (133), Expect = 2e-09
Identities = 35/135 (25%), Positives = 68/135 (50%)
Frame = +1
Query: 103 NEEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFTRRNLAE 282
N +K++ IS + G + DT I+ NI+L+ + + A + +++L
Sbjct: 65 NCKKLHLISPNIWCAGAGTAADTEFVTSMISSNIELHSLYTNRKPRVVTALTMLKQHLFR 124
Query: 283 YLRSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKD 462
Y Y V +GGYD + GP L+ + SS K+P+ A G G ++S+++ ++ D
Sbjct: 125 YQGHIGAYLV---LGGYDCK-GPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPD 180
Query: 463 ATETEAYDILKKCVQ 507
EA +++K+ ++
Sbjct: 181 LERHEAMELVKEAIE 195
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 44.0 bits (99), Expect = 3e-05
Identities = 25/92 (27%), Positives = 43/92 (46%)
Frame = +1
Query: 106 EEKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFTRRNLAEY 285
+ +++++ D LV+G G D + I + I LY + ++ + A R L Y
Sbjct: 44 QPRVHEVGDDLVIGASGFEADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLL--Y 101
Query: 286 LRSSTPYFVNVLMGGYDKENGPELYFMDYLAS 381
+ PY+V + G DKE E+Y D + S
Sbjct: 102 GKRFFPYYVYTTVAGIDKEGKGEIYSFDPVGS 133
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 36.7 bits (81), Expect = 0.004
Identities = 40/163 (24%), Positives = 67/163 (41%), Gaps = 8/163 (4%)
Frame = +1
Query: 109 EKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQ----LYKMRNGYELGPSA----AASFT 264
EK+++I + + G + D E+ Q Y G E + A F
Sbjct: 65 EKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFG 124
Query: 265 RRNLAEYLRSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAHGYGGYLSLSIMD 444
E S P+ V +L+ G D E+GP+LY + + + A G G + S +
Sbjct: 125 EGEDGEERIMSRPFGVALLIAGID-EHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELV 183
Query: 445 RYHKKDATETEAYDILKKCVQEVHKRLFVSLPNFQVTVVNRDG 573
+ KD T EA ++ K +++V + S N Q+ V +G
Sbjct: 184 KEFHKDMTLEEAEVLILKVLRQVMEEKLDS-KNVQLAKVTAEG 225
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 35.5 bits (78), Expect = 0.010
Identities = 28/132 (21%), Positives = 62/132 (46%)
Frame = +1
Query: 109 EKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFTRRNLAEYL 288
+K+ +I+ L+ + G + D + + +L+++RN EL +AAS N+ Y
Sbjct: 93 KKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRN-KELISVSAASKILSNIT-YS 150
Query: 289 RSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAHGYGGYLSLSIMDRYHKKDAT 468
+ ++ G K G LY++D + +K + G G + ++D ++ D +
Sbjct: 151 YKGYGLSMGTMLAGTGK-GGTALYYIDSDGTRLKGDLFSVGSGSTFAYGVLDSGYRWDLS 209
Query: 469 ETEAYDILKKCV 504
+ EA + ++ +
Sbjct: 210 KQEALYLAQRSI 221
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 27.9 bits (59), Expect = 2.0
Identities = 34/162 (20%), Positives = 63/162 (38%), Gaps = 5/162 (3%)
Frame = +1
Query: 109 EKIYKISDRLVMGVIGDSGDTNQFAEYIAKNIQLYKMRNGYELGPSAAASFT---RRNLA 279
EK+Y I D ++ V G + D N Y + Q Y E+ ++
Sbjct: 63 EKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYT 122
Query: 280 EYLRSSTPYFVNVLMGGYDKENGPELYFMDYLASSVKVPFAAHGYGGYLS--LSIMDRYH 453
+Y P+ V+ L G+D G +L+ + S + A+ GG + S+M + +
Sbjct: 123 QY-GGLRPFGVSFLYAGWDHIRGYQLFQSN--PSGNYGSWQANSIGGNSTSVQSLMRQEY 179
Query: 454 KKDATETEAYDILKKCVQEVHKRLFVSLPNFQVTVVNRDGIK 579
K D EA + K + + ++ + + +D K
Sbjct: 180 KDDINLDEASAMAVKFLSKTLDSNSLTHEKIEFATITKDTTK 221
>SPAC9.09 |met26||homocysteine methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 764
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +2
Query: 146 VSLVTQETQINLLNILQKIFSCIKCVMGMNWGPQLQPVS 262
VS+ ++ + LLN+L + SCI + P++ PVS
Sbjct: 673 VSIENSKSDMKLLNVLSRYTSCIGPGLFDIHSPRVPPVS 711
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,947,871
Number of Sequences: 5004
Number of extensions: 57427
Number of successful extensions: 155
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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