BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_M16
(901 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.11 |atp17||F0 ATPase subunit F|Schizosaccharomyces pomb... 29 1.2
SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulat... 29 1.2
SPBC21C3.03 |||ABC1 kinase family protein|Schizosaccharomyces po... 28 1.6
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 28 2.1
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 27 2.7
SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomy... 27 3.6
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 27 4.8
SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH comple... 27 4.8
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 27 4.8
SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces po... 27 4.8
SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.3
SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch... 26 6.3
SPCC548.07c |ght1||hexose transporter Ght1 |Schizosaccharomyces ... 26 8.4
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 26 8.4
SPAC11D3.17 |||zinc finger protein|Schizosaccharomyces pombe|chr... 26 8.4
>SPBC1604.11 |atp17||F0 ATPase subunit F|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 96
Score = 28.7 bits (61), Expect = 1.2
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +2
Query: 401 PWPDEDEVSPIVDND---NVFMILYKELYYRDIYARVPGGPKPEQRFHSFYNY 550
P+P + + P V N N F + D Y+R+P GP P++ +SF+++
Sbjct: 3 PFPLKSFIPPQVANPTALNAFPSSARMGRIVDFYSRLPHGPAPKKSSNSFFSW 55
>SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulatory
subunit Rpn11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 308
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 770 VDKSNIKRQLEVYASGGDPDSVAGEFGRHPWYKCWDTS 883
VD K +++ G P+ V G + HP + CW +S
Sbjct: 86 VDPVFQKNMMDMLKQTGRPEMVVGWYHSHPGFGCWLSS 123
>SPBC21C3.03 |||ABC1 kinase family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 674
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 5/54 (9%)
Frame = -1
Query: 715 IKSFNFSWTQLCGTRPVLG---KRLELIN--KFINKLPQPLVGQLERYRSYRED 569
+ S N W +LCGT+ V K+ L+N K I+ P+P+ + R ED
Sbjct: 21 VNSQNTRWKRLCGTQSVWSPYKKKHVLVNRLKHISLFPKPIFARKFTTRQKSED 74
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/41 (26%), Positives = 21/41 (51%)
Frame = +2
Query: 317 INEGVTYEILNLYENTFPTLTEKYFENTPWPDEDEVSPIVD 439
I+ G YE++NL ++ +T+ + PW D+ +D
Sbjct: 431 IDGGCVYEVINLVQSVLRRVTKLFQLKEPWKLSDDSQEKID 471
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 27.5 bits (58), Expect = 2.7
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +2
Query: 281 WSRNSLXYFRNMINEGVTYEILNLYEN-TFPTLTEKYFENTPWPDEDEVSPIVDNDNVFM 457
W+ N+ F N I G + +EN TF + +K EN+P P++ + P + M
Sbjct: 262 WNENA-GVFINNIYFGFSLSDAMKFENETF--IAKKILENSPCPNQPSIQPF----GILM 314
Query: 458 ILYKELY--YRDIY 493
+L +Y ++++Y
Sbjct: 315 MLVSTIYGNFKNLY 328
>SPAC11H11.04 |mam2||pheromone p-factor receptor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 348
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/40 (35%), Positives = 25/40 (62%)
Frame = +3
Query: 735 TYCASSMFCTLSWTSLILNVSLRYMHLVVILILWLASLVV 854
T C++S +++ ILN+ Y+H+ ILIL LA +++
Sbjct: 100 TICSNSYSILVNY-GFILNMVHMYVHVFNILILLLAPVII 138
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 26.6 bits (56), Expect = 4.8
Identities = 17/46 (36%), Positives = 19/46 (41%)
Frame = -1
Query: 283 PCQAFYNKKTCHIPARSVLWTVLHSRHRIRNLPHYNRRWNTWLLIE 146
PC+A N CHI R+ W R P Y NT L IE
Sbjct: 59 PCKAVLNPY-CHIDTRAKFWICPFCLQRNMLPPQYKDISNTSLPIE 103
>SPBC13G1.13 |tfb2|SPBC31F10.01|transcription factor TFIIH complex
subunit Tfb2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 447
Score = 26.6 bits (56), Expect = 4.8
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = +2
Query: 566 YILSV--TPVPLELPDQWLWELIDEFVYQFQSF 658
Y++S+ P+P+ L D LW + VYQ +SF
Sbjct: 43 YVMSMLFNPMPVALSDFDLWIKLSSKVYQSESF 75
>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/42 (26%), Positives = 23/42 (54%)
Frame = +3
Query: 408 QMKMRSHLLWTMIMYS*SYTKNYTTATSMQESLEDQNQSSAS 533
Q S +W + ++ K+YT AT ++ +EDQ + +++
Sbjct: 431 QFPFESRKIWFPVTHN-ILAKHYTQATKAKQEIEDQQRQASA 471
>SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 467
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -1
Query: 232 VLWTVLHSRHRIRNLPHYNRRWN 164
+L H+R I +LPH+ RRW+
Sbjct: 407 ILQLATHNRSFICSLPHFLRRWS 429
>SPAC4D7.07c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 600
Score = 26.2 bits (55), Expect = 6.3
Identities = 19/81 (23%), Positives = 39/81 (48%)
Frame = +2
Query: 533 HSFYNYCDLFNYILSVTPVPLELPDQWLWELIDEFVYQFQSFAQYRSRTTKLSPAEIEAL 712
+++ ++ + Y++S PV + P L+++ V SFA++ + S +
Sbjct: 235 NTYLDFIEKDIYLVSHLPVSEKHPGNIPISLVNKSVQAICSFAEHYNLLRNPSYRGFLRI 294
Query: 713 NTENKAWNVLCILNVLHSLVD 775
N +N+LCI + LH V+
Sbjct: 295 NNGESIFNLLCIED-LHESVN 314
>SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein
Rrp12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1001
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/43 (27%), Positives = 26/43 (60%)
Frame = +2
Query: 311 NMINEGVTYEILNLYENTFPTLTEKYFENTPWPDEDEVSPIVD 439
+MI+ + + LNL +T LT ++E + + D+D V+ +++
Sbjct: 795 SMISANLMNDSLNLKVSTIFALTRIFYEFSDYIDDDYVNAVLE 837
>SPCC548.07c |ght1||hexose transporter Ght1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 557
Score = 25.8 bits (54), Expect = 8.4
Identities = 10/35 (28%), Positives = 19/35 (54%)
Frame = +1
Query: 160 MYSSDDYNEGGYESYGDYEAQSTEHYEPEYDRSSY 264
M+ +D + GG + D++++ + Y P D SY
Sbjct: 19 MFGADTGSIGGITNMRDFQSRFADRYNPVTDTYSY 53
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/55 (27%), Positives = 25/55 (45%), Gaps = 1/55 (1%)
Frame = +2
Query: 542 YNYCDLFNYILSVTPVPLELPDQWLWELIDEFVYQF-QSFAQYRSRTTKLSPAEI 703
Y C ++++ +T VPL WL + Y F ++ A Y + L+ A I
Sbjct: 396 YGLCAFSSFLVVITKVPLHFLPTWLRHVSRIVYYMFSKTSASYVTSPLTLAYASI 450
>SPAC11D3.17 |||zinc finger protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 585
Score = 25.8 bits (54), Expect = 8.4
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +2
Query: 386 YFENTPWPDEDEVSPIVDNDNVFM 457
YF++ W D+D +SP + N+ M
Sbjct: 451 YFKDDSWMDDDYISPCMTTYNLTM 474
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,692,900
Number of Sequences: 5004
Number of extensions: 78382
Number of successful extensions: 273
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 273
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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