Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP21_F_M04
         (892 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa...   143   3e-35
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S...    27   2.7  
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch...    26   6.3  

>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score =  143 bits (346), Expect = 3e-35
 Identities = 89/233 (38%), Positives = 122/233 (52%), Gaps = 6/233 (2%)
 Frame = +3

Query: 147 FKKIAVVPTAKDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIRKVKYTQQNFHDRLSRI 326
           FK I  +P    F+D++LS+TQRKTPTV+   +KISRIRGFY RKVK+TQ    ++L  I
Sbjct: 6   FKNITPIPDVNTFLDVVLSRTQRKTPTVIRSGFKISRIRGFYGRKVKFTQDTITEKLDSI 65

Query: 327 IQEFPKLDDVHPFYADLMNVLYDKGSL---XTWTRTAEHXQTSY*QCCKDYVRLVKYXDS 497
           +QEFPKL+D+HPF+ADL+N+LYD+  L    +   TA+H   +     +DY+RL+KY DS
Sbjct: 66  LQEFPKLNDIHPFHADLLNILYDRDHLKIALSQLSTAKHLVEN---VARDYIRLLKYGDS 122

Query: 498 LYPCNS-*NVXLXVXXALS*NXXGPIXPSWNXXVXIXHVYRR*XPTPGR*SSV--GXPTF 668
           LY C       L     +       +   +   V   H+ R     P   + +  G P  
Sbjct: 123 LYRCKQLKRAALGRMATIIKRQKSSL--EFLEQVR-QHLSRLPAIDPNTRTLLVCGYPNV 179

Query: 669 GKPVXXXXXXXXXXEMXPFAFTXKXLDXGPPDYXXLGMGGEKPPGXPDSPWEK 827
           GK            ++ P+AFT K L  G  DY  L       PG  D P E+
Sbjct: 180 GKSSFMNKVTRAQVDVQPYAFTTKSLFVGHFDYKYLRWQVIDTPGILDHPLEQ 232



 Score = 68.9 bits (161), Expect = 9e-13
 Identities = 34/63 (53%), Positives = 42/63 (66%)
 Frame = +2

Query: 509 QQLKRAALGXXGPIMKXAXAYXTFLEXVRXHLXRLPAIDXYXRTIIICXXPNVRKTSFIX 688
           +QLKRAALG    I+K   +   FLE VR HL RLPAID   RT+++C  PNV K+SF+ 
Sbjct: 127 KQLKRAALGRMATIIKRQKSSLEFLEQVRQHLSRLPAIDPNTRTLLVCGYPNVGKSSFMN 186

Query: 689 KKT 697
           K T
Sbjct: 187 KVT 189


>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1944

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 13/39 (33%), Positives = 21/39 (53%)
 Frame = +3

Query: 153  KIAVVPTAKDFIDIILSKTQRKTPTVVHKHYKISRIRGF 269
            KI VV   +  +  + S+ QRK  +++ KH  I  + GF
Sbjct: 1670 KIGVVTPYRSQVQQLRSQFQRKYGSIIFKHLDIHTVDGF 1708


>SPAP8A3.14c |||mitochondrial inner membrane protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 677

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 23/90 (25%), Positives = 43/90 (47%), Gaps = 3/90 (3%)
 Frame = +3

Query: 129 KMSLYNFKKIAVVPTAKDFIDIILSKTQRKTPTVVHKHYKISRIRGFYIR-KVKYTQQNF 305
           K++   FK IA   +   F+D  +S   R   T   +  +++   GF     +K +   F
Sbjct: 547 KINTTTFKTIANNKSVDAFLDDCVSFFSRPEMT---QDSQLNASSGFLTNFSLKDSTDRF 603

Query: 306 HDRLS--RIIQEFPKLDDVHPFYADLMNVL 389
           +  LS  ++ + F K+DD +  Y+D+ + L
Sbjct: 604 YKVLSYEKVSERFVKIDDSYITYSDIFSPL 633


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,061,821
Number of Sequences: 5004
Number of extensions: 57233
Number of successful extensions: 125
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)