Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_L24
(932 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein L23|Schizosac... 159 7e-40
SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein L23|Schizosacc... 159 7e-40
SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit L38|... 47 4e-06
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 26 6.6
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 26 8.7
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 8.7
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 8.7
>SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 159 bits (385), Expect = 7e-40
Identities = 70/84 (83%), Positives = 80/84 (95%)
Frame = +1
Query: 223 RLNRLPAAGSGDMIVATVKKGKPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVN 402
RLNRLPAA GDM++ATVKKGKP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN
Sbjct: 47 RLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVN 106
Query: 403 NKGEMKGSAITGPVAKECADLWPR 474
KGEMKGSAITGPVAKECADLWPR
Sbjct: 107 PKGEMKGSAITGPVAKECADLWPR 130
Score = 27.5 bits (58), Expect = 2.9
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
Frame = +2
Query: 86 RXRGGSCGSEIPYLPGXSQXGAVINCADNTGCKE----SVCDRCPRXSKVA*TDCRRPVL 253
R RG + G++ G A++NCADN+G K SV R +++ C VL
Sbjct: 3 RGRGAASGTKYRMTLGLPVQ-AIMNCADNSGAKNLYIVSVFGTGARLNRLPAASCGDMVL 61
Query: 254 GT 259
T
Sbjct: 62 AT 63
>SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 139
Score = 159 bits (385), Expect = 7e-40
Identities = 70/84 (83%), Positives = 80/84 (95%)
Frame = +1
Query: 223 RLNRLPAAGSGDMIVATVKKGKPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVN 402
RLNRLPAA GDM++ATVKKGKP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN
Sbjct: 47 RLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVN 106
Query: 403 NKGEMKGSAITGPVAKECADLWPR 474
KGEMKGSAITGPVAKECADLWPR
Sbjct: 107 PKGEMKGSAITGPVAKECADLWPR 130
Score = 27.5 bits (58), Expect = 2.9
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 4/62 (6%)
Frame = +2
Query: 86 RXRGGSCGSEIPYLPGXSQXGAVINCADNTGCKE----SVCDRCPRXSKVA*TDCRRPVL 253
R RG + G++ G A++NCADN+G K SV R +++ C VL
Sbjct: 3 RGRGAASGTKYRMTLGLPVQ-AIMNCADNSGAKNLYIVSVFGTGARLNRLPAASCGDMVL 61
Query: 254 GT 259
T
Sbjct: 62 AT 63
>SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit
L38|Schizosaccharomyces pombe|chr 2|||Manual
Length = 126
Score = 46.8 bits (106), Expect = 4e-06
Identities = 28/79 (35%), Positives = 41/79 (51%), Gaps = 9/79 (11%)
Frame = +1
Query: 244 AGSGDMIVATVKKGKPEL---------RKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVI 396
A GD +V VKK + R + A+++R + P RR DG ++ F+DNA V+
Sbjct: 31 ASLGDEVVVVVKKARSGSSVTAANKVKRGDIHHAIIVRTKSPVRRPDGRYVRFDDNACVL 90
Query: 397 VNNKGEMKGSAITGPVAKE 453
VN + E G+ I VA E
Sbjct: 91 VNKECEPLGTRILSVVANE 109
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 26.2 bits (55), Expect = 6.6
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +1
Query: 277 KKGKPELRKKVMPAVVIRQRK---PFRRRDGVFIYFEDNA 387
KKG P RK P+++ PF RD +FI E NA
Sbjct: 337 KKGAPVFRKH-FPSILQELNSLDPPFYERDAIFIVDEGNA 375
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.8 bits (54), Expect = 8.7
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = -1
Query: 425 EPFISPLLLTMTPALSSKYINTPS 354
+P+ P+L + P L Y++ PS
Sbjct: 275 QPYSGPILASTAPTLDGSYLSNPS 298
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = -1
Query: 467 HRSAHSLATGPVMAEPFISPLLLTMTPALSSKYINTPSRLLNGFRCLMTTA 315
+ + H GP++ ++ L P +S Y+N + L+GF+ ++ A
Sbjct: 963 YNATHFEHIGPMLEATWMPILAALSNPLQNSDYVNELNMCLDGFQLVVRIA 1013
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = -1
Query: 473 RGHRSAHSLATGPVMAEPFISPLLL-TMTPALSSKYINTPSRLLNGF 336
R H+ AH+ AEP ++P++ + P+++ +TPS L F
Sbjct: 60 RRHKDAHAYDMSLTDAEPALAPVMSPAIPPSMNYPTPSTPSVLPESF 106
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,627,909
Number of Sequences: 5004
Number of extensions: 46647
Number of successful extensions: 106
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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