Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_L04
(900 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 65 1e-11
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 56 9e-09
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 45 2e-05
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 33 0.055
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 28 2.1
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 27 3.6
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 26 6.3
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 65.3 bits (152), Expect = 1e-11
Identities = 41/158 (25%), Positives = 58/158 (36%), Gaps = 1/158 (0%)
Frame = +1
Query: 400 YESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGN 579
Y C C + + +S K H CF C C +L F + K YC
Sbjct: 250 YRGNSEKSCHSCGGSLRA-GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 580 CYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTX-SFIPREQXIYC 756
Y QF+ RC C + + +HE C C I R+ +C
Sbjct: 309 DYHEQFSPRCKHCKTPIE--DQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWC 366
Query: 757 AGCYEDKFATRCVKCNKIITQGGVTYXNXPXXXXCFTC 870
CY++K+A +C KC K I V + C+TC
Sbjct: 367 QTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTC 404
Score = 59.7 bits (138), Expect = 6e-10
Identities = 40/165 (24%), Positives = 65/165 (39%), Gaps = 1/165 (0%)
Frame = +1
Query: 274 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKCYESVFANGCEECNKIIGI 453
G A K H F C C ++L + R+ YC Y F+ C+ C I
Sbjct: 268 GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCK--TPI 325
Query: 454 DSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVF 630
+ + + + +HE CA C V V+ + D +C CYD ++A +C C +
Sbjct: 326 EDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPI 385
Query: 631 RAGTKKMEYKTRQWHEKCFCCVVCKNPIGTXSFIPREQXIYCAGC 765
+ K ++H +C+ C C +G + E C C
Sbjct: 386 LGISVK--GSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 55.6 bits (128), Expect = 9e-09
Identities = 33/104 (31%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
Frame = +1
Query: 304 WHSGHFCCWQCDESLTGQRYVL---RDEHPYCIKCYESVFANGCEECNKIIGIDSKDLSY 474
WH FCC +CD+ L +L D P C C A+ C C I D +S
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIK-DYALMSG 93
Query: 475 KDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASR 606
D + H CF C CR ++D F I+C +C + SR
Sbjct: 94 YDSY-HRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
Score = 44.0 bits (99), Expect = 3e-05
Identities = 30/125 (24%), Positives = 48/125 (38%), Gaps = 3/125 (2%)
Frame = +1
Query: 424 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKLD-KIYCGNCYDAQ 594
C C + +S+ WH+ CF C KC L D+ D + C +C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74
Query: 595 FASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTXSFIPREQXIYCAGCYED 774
A C C + Y + +H +CF C C+ I +F + I+C C +
Sbjct: 75 -AHTCTACRMRIKDYALMSGYDS--YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQV 131
Query: 775 KFATR 789
+ +R
Sbjct: 132 RHPSR 136
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 44.8 bits (101), Expect = 2e-05
Identities = 35/145 (24%), Positives = 58/145 (40%), Gaps = 8/145 (5%)
Frame = +1
Query: 274 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRD-----EHPYCIKCYESVFANGCEECN 438
G+Y +A+ +H F C C+ + + + + D + P C Y C C
Sbjct: 125 GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCG 184
Query: 439 KIIGIDSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDG 615
+ + ++ +K +H F C+ C V + + K+YC Y FA+RC G
Sbjct: 185 --MALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCG 242
Query: 616 C-GEVFRAGTKKMEYKTRQ-WHEKC 684
C G + R + Q WH C
Sbjct: 243 CDGPILRQFVEVYRNGVSQNWHVPC 267
Score = 41.9 bits (94), Expect = 1e-04
Identities = 37/146 (25%), Positives = 55/146 (37%), Gaps = 7/146 (4%)
Frame = +1
Query: 424 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQF---GSKLDKIY--CGNCYD 588
C C ++I + + +H CF C C + K F L+K C Y
Sbjct: 116 CASCGQVIS--GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYF 173
Query: 589 AQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIG-TXSFIPREQXIYCAGC 765
+ C CG R + +++H + F C +C G S+ E +YC
Sbjct: 174 RRLDLLCASCGMALRG--YYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYH 231
Query: 766 YEDKFATRCVKCN-KIITQGGVTYXN 840
Y FA RC C+ I+ Q Y N
Sbjct: 232 YSTLFAARCCGCDGPILRQFVEVYRN 257
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 33.1 bits (72), Expect = 0.055
Identities = 34/131 (25%), Positives = 49/131 (37%), Gaps = 4/131 (3%)
Frame = +1
Query: 385 YCIKCYESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKL 558
+CIKC+ESV + + + + K WH CF C C L + F
Sbjct: 23 FCIKCWESVPS-------------TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69
Query: 559 DK-IYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCF-CCVVCKNPIGTXSFI 732
K I+C C D C+GC T E+ + CC C+ I + +F
Sbjct: 70 QKQIFCKLCVDI-----CNGC------STPICEFNAVSNSQANHPCCSNCRAFINSNAFG 118
Query: 733 PREQXIYCAGC 765
+ YC C
Sbjct: 119 KFKGQHYCLPC 129
Score = 27.9 bits (59), Expect = 2.1
Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 3/102 (2%)
Frame = +1
Query: 298 KDWHSGHFCCWQCDESL--TGQRYVLRDEHP-YCIKCYESVFANGCEECNKIIGIDSKDL 468
K WHS F C C++ L + + + D+ +C C + NGC I +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD--ICNGCS--TPICEFN---- 93
Query: 469 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQ 594
+ + + C C+ CR + FG + YC C Q
Sbjct: 94 AVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQ 133
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 3/58 (5%)
Frame = +1
Query: 607 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTXS--FIPREQ-XIYCAGCYE 771
C C E + T ++ + + WH CF CV C + S F +Q I+C C +
Sbjct: 24 CIKCWESVPS-TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD 80
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +1
Query: 313 GHFCCWQCDESLTGQRYVLRDEHPYCIKC 399
GHF C +C L GQ+Y KC
Sbjct: 623 GHFTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 27.1 bits (57), Expect = 3.6
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -2
Query: 536 STRETRHLAHKKQASCQCLSLYDRSLESMPMILLHSSQPLAKTLS 402
S R T A KQA L+ Y +++M IL + +PLA T S
Sbjct: 753 SPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRS 797
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 26.2 bits (55), Expect = 6.3
Identities = 22/82 (26%), Positives = 30/82 (36%)
Frame = +1
Query: 472 YKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVFRAGTKKM 651
Y H C++CAKC+ L K G L Y G A R + V K
Sbjct: 4 YYPVHLKSRCYVCAKCKTHLAFK--GHLLSHDYRGKNGPACLFKRVE---NVIEMEPKTE 58
Query: 652 EYKTRQWHEKCFCCVVCKNPIG 717
+ T ++ + C C IG
Sbjct: 59 QMSTGRFIVRHIHCCRCHTYIG 80
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,667,212
Number of Sequences: 5004
Number of extensions: 46036
Number of successful extensions: 155
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -