BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_K24
(972 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.061
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.080
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 1.7
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.0
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 9.2
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.061
Identities = 23/71 (32%), Positives = 24/71 (33%), Gaps = 5/71 (7%)
Frame = -1
Query: 462 PPPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPP---TXXQKXXXAPXGXGGPXXPPXXP 292
PPPPPPR P P R + PPAPP P G P P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAV--SNPPAPPPAIPGRSAPALPPLGNASRTSTPPVP 418
Query: 291 X*GGXPP--PP 265
PP PP
Sbjct: 419 TPPSLPPSAPP 429
Score = 32.7 bits (71), Expect = 0.080
Identities = 23/75 (30%), Positives = 25/75 (33%), Gaps = 5/75 (6%)
Frame = -1
Query: 459 PPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPPTXXQ---KXXXAPXGXGGPXXPP--XX 295
PPPPP + + P P PP PP P G P PP
Sbjct: 311 PPPPPPPSRRNRGKP-PIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSA 369
Query: 294 PX*GGXPPPPXXSXA 250
P G PPP S A
Sbjct: 370 PSTGRQPPPLSSSRA 384
Score = 31.9 bits (69), Expect = 0.14
Identities = 21/71 (29%), Positives = 22/71 (30%)
Frame = -1
Query: 462 PPPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPPTXXQKXXXAPXGXGGPXXPPXXPX*G 283
P P PP P P G P APP P G P PP P
Sbjct: 416 PVPTPPSLPPSAPPSLPPSAPPSLPMGA-PAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 282 GXPPPPXXSXA 250
PP P + A
Sbjct: 475 APPPAPAPAPA 485
Score = 29.5 bits (63), Expect = 0.75
Identities = 16/47 (34%), Positives = 17/47 (36%)
Frame = -2
Query: 500 PXXGXXXXXXXXPPPPPPXGXXKXXXXPXXPGPRAGXXGGPXPXPPR 360
P G PPPPPP P P R+ P P PPR
Sbjct: 325 PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRS----APPPPPPR 367
Score = 26.6 bits (56), Expect = 5.3
Identities = 13/35 (37%), Positives = 14/35 (40%)
Frame = -2
Query: 464 PPPPPPXGXXKXXXXPXXPGPRAGXXGGPXPXPPR 360
PPPPPP P G + P P PPR
Sbjct: 311 PPPPPPPSRRNRGKPPIGNG-SSNSSLPPPPPPPR 344
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.080
Identities = 15/36 (41%), Positives = 15/36 (41%)
Frame = -2
Query: 464 PPPPPPXGXXKXXXXPXXPGPRAGXXGGPXPXPPRP 357
PPPPP P P A GGP P PP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 32.7 bits (71), Expect = 0.080
Identities = 15/36 (41%), Positives = 15/36 (41%)
Frame = -2
Query: 464 PPPPPPXGXXKXXXXPXXPGPRAGXXGGPXPXPPRP 357
PPPPPP G P P P A GG P P
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 31.9 bits (69), Expect = 0.14
Identities = 22/65 (33%), Positives = 22/65 (33%)
Frame = -1
Query: 462 PPPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPPTXXQKXXXAPXGXGGPXXPPXXPX*G 283
PPPPP P P P G PP PP P G G PP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPP---------PPGVAGAGPPPPPP--- 780
Query: 282 GXPPP 268
PPP
Sbjct: 781 --PPP 783
Score = 29.9 bits (64), Expect = 0.57
Identities = 15/49 (30%), Positives = 15/49 (30%)
Frame = -2
Query: 503 PPXXGXXXXXXXXPPPPPPXGXXKXXXXPXXPGPRAGXXGGPXPXPPRP 357
PP P P PP P P P G P P PP P
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.5 bits (58), Expect = 3.0
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = -3
Query: 307 PPXXPXLGGXPPXPP 263
PP P +GG PP PP
Sbjct: 752 PPPAPIMGGPPPPPP 766
Score = 26.6 bits (56), Expect = 5.3
Identities = 14/48 (29%), Positives = 14/48 (29%)
Frame = -1
Query: 504 PPGXGXXXXXXXXXPPPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPP 361
PP P P PP P P P G PP PP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 26.6 bits (56), Expect = 5.3
Identities = 13/37 (35%), Positives = 13/37 (35%)
Frame = -1
Query: 375 PPAPPTXXQKXXXAPXGXGGPXXPPXXPX*GGXPPPP 265
P P AP G P PP G PPPP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.3 bits (60), Expect = 1.7
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = -1
Query: 462 PPPPPPRGXKXXKXXPXPXXP 400
PPPPPP G + P P P
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
Score = 27.5 bits (58), Expect = 3.0
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = -3
Query: 463 PPPPPPXGXXXKXXXPXPXXP 401
PPPPPP G P P P
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.5 bits (58), Expect = 3.0
Identities = 23/69 (33%), Positives = 23/69 (33%), Gaps = 4/69 (5%)
Frame = +2
Query: 266 GGGGXPP---*XGXXGGXXGPPXPXGAXXXFCXXVGG-AGGXVPPXXRXGXXGXGXXXLX 433
G GG PP G GG G G F GG GG G G G
Sbjct: 196 GSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFG 255
Query: 434 XXPRGGGGG 460
P G GGG
Sbjct: 256 GGPGGFGGG 264
Score = 26.2 bits (55), Expect = 7.0
Identities = 14/36 (38%), Positives = 14/36 (38%)
Frame = +1
Query: 358 GRGGXGXGPPXXPARGPGXXGXFXXFXXPXGGGGGG 465
G GG G GP G G F GG GGG
Sbjct: 222 GHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGG 257
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 9.2
Identities = 19/71 (26%), Positives = 20/71 (28%)
Frame = -1
Query: 462 PPPPPPRGXKXXKXXPXPXXPXRXXGGTXPPAPPTXXQKXXXAPXGXGGPXXPPXXPX*G 283
PP P P G P G P P+ AP PP
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSG----IPPVPKPAA 1186
Query: 282 GXPPPPXXSXA 250
G PP P S A
Sbjct: 1187 GVPPVPPPSEA 1197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.312 0.160 0.582
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,389,918
Number of Sequences: 5004
Number of extensions: 19511
Number of successful extensions: 116
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 499296500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 43 (22.0 bits)
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