BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_K22
(956 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.14
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.14
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 1.3
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 28 1.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.8
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.9 bits (69), Expect = 0.14
Identities = 13/22 (59%), Positives = 13/22 (59%), Gaps = 2/22 (9%)
Frame = +3
Query: 897 PPXGXGPPPPXXGFXPP--PPP 956
PP PPPP GF PP PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPP 26
Score = 26.6 bits (56), Expect = 5.2
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +2
Query: 434 PPPPXGXGFXFXGPXXXPPPXTPG 505
PPPP GF P PPP PG
Sbjct: 10 PPPPPPPGFE--PPSQPPPPPPPG 31
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.9 bits (69), Expect = 0.14
Identities = 21/74 (28%), Positives = 21/74 (28%), Gaps = 1/74 (1%)
Frame = +2
Query: 425 LXXPPPPXGXGFXFXGPXXXPPPXTPGPXXSGGGXXXPPXPG-GXLGXPXPXGSPXEXFF 601
L PPP P P P P GG PP PG G P P P
Sbjct: 728 LLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSA 787
Query: 602 XXLXPXGPXPXKRP 643
P P P
Sbjct: 788 GGSRYYAPAPQAEP 801
Score = 31.1 bits (67), Expect = 0.24
Identities = 15/50 (30%), Positives = 17/50 (34%)
Frame = +3
Query: 438 PPPGXGXXLFXAXSXXPXPXXRAPGXRGGXXXXPPPQGXXWVXPPXGGPP 587
PPP + + P P GG PPP G PP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 31.1 bits (67), Expect = 0.24
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = -3
Query: 573 GXGXPKXPPGXGGXXXPPPXSXGPGVXGGG 484
G P PPG G PPP P V GG
Sbjct: 760 GPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 29.5 bits (63), Expect = 0.73
Identities = 16/33 (48%), Positives = 17/33 (51%)
Frame = +3
Query: 858 GGXFPXXPXXGXSPPXGXGPPPPXXGFXPPPPP 956
GG P P G + G GPPPP PPPPP
Sbjct: 759 GGPPPPPPPPGVA---GAGPPPP-----PPPPP 783
Score = 28.3 bits (60), Expect = 1.7
Identities = 17/52 (32%), Positives = 19/52 (36%), Gaps = 3/52 (5%)
Frame = +1
Query: 613 PPXPXXXKTPPFXGFFPXXPP-PLXX--PXXXPPPXLXGGAPPXXXFKXPRV 759
PP P P P PP P+ P PPP + G PP P V
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAV 785
Score = 27.9 bits (59), Expect = 2.2
Identities = 15/32 (46%), Positives = 15/32 (46%), Gaps = 3/32 (9%)
Frame = +3
Query: 870 PXXPXXGXSPPXGXGPPPPXX---GFXPPPPP 956
P P G PP PPPP G PPPPP
Sbjct: 753 PPAPIMGGPPPP---PPPPGVAGAGPPPPPPP 781
Score = 26.6 bits (56), Expect = 5.2
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +3
Query: 900 PXGXGPPPPXXGFXPPPPP 956
P PP P G PPPPP
Sbjct: 748 PIPVPPPAPIMGGPPPPPP 766
Score = 25.8 bits (54), Expect = 9.0
Identities = 17/59 (28%), Positives = 19/59 (32%)
Frame = -3
Query: 561 PKXPPGXGGXXXPPPXSXGPGVXGGGXXXGPXKXXPXPXGGGGXFXRXKXXXXPPRXRE 385
P P GG PPP PGV G G P GG + P+ E
Sbjct: 752 PPPAPIMGG---PPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEPKIDE 807
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.7 bits (61), Expect = 1.3
Identities = 20/74 (27%), Positives = 21/74 (28%)
Frame = +2
Query: 422 RLXXPPPPXGXGFXFXGPXXXPPPXTPGPXXSGGGXXXPPXPGGXLGXPXPXGSPXEXFF 601
R PP P P PP P G P P + P P G P
Sbjct: 411 RTSTPPVPTPPSLPPSAPPSLPPSAPPS-LPMGAPAAPPLPPSAPIAPPLPAGMPAA--- 466
Query: 602 XXLXPXGPXPXKRP 643
L P P P P
Sbjct: 467 PPLPPAAPAPPPAP 480
Score = 27.9 bits (59), Expect = 2.2
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = +3
Query: 849 GGGGGXFPXXPXXGXSPPXGXGPPPPXXGFXPPPPP 956
G P P S G P PP PPPPP
Sbjct: 330 GSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP 365
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.3 bits (60), Expect = 1.7
Identities = 15/39 (38%), Positives = 17/39 (43%)
Frame = -1
Query: 755 RGXLKXXXGGAPPXRXGGGXXXGXXRGGGXFGKXPXKGG 639
RG + GG R G G G RGGG G +GG
Sbjct: 11 RGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGG 49
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 3.0
Identities = 34/122 (27%), Positives = 36/122 (29%), Gaps = 7/122 (5%)
Frame = +1
Query: 388 PXPGGGXXXFXPXKXTPP-PXGXGXXFXGPXPLXPXXXXGPXGXGGGXXXXPXPRGXFGX 564
P P G P PP P G P P P P G P P
Sbjct: 1115 PAPSGAPPVPKPSVAAPPVPVPSGAP---PVP-KPSVAAPPVPAPSGAPPVPKP-SVAAP 1169
Query: 565 PXPX---GVPXGXIFXXXXPPXPXXXKTPPFXG---FFPXXPPPLXXPXXXPPPXLXGGA 726
P P G+P PP P + PP P PPP P P P G
Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAP---PVPTPSAGL 1226
Query: 727 PP 732
PP
Sbjct: 1227 PP 1228
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.8
Identities = 14/40 (35%), Positives = 15/40 (37%)
Frame = +1
Query: 613 PPXPXXXKTPPFXGFFPXXPPPLXXPXXXPPPXLXGGAPP 732
PP P PP P PP P PPP L + P
Sbjct: 1707 PPPPPMSVPPP-----PSAPPMPAGPPSAPPPPLPASSAP 1741
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,610,913
Number of Sequences: 5004
Number of extensions: 45305
Number of successful extensions: 196
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 44
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 489310570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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