Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP21_F_J22
         (810 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo...   113   4e-26
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo...   101   1e-22
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa...    31   0.26 
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22...    27   2.4  
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual        27   4.2  
SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4...    26   7.3  
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy...    25   9.6  

>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 472

 Score =  113 bits (271), Expect = 4e-26
 Identities = 58/129 (44%), Positives = 75/129 (58%)
 Frame = +1

Query: 151 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPN 330
           KLL + L E DP ++ I+  EK RQ+  + +IASENFTS  V+  L S + NKYSEG P 
Sbjct: 12  KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71

Query: 331 QRYYGGNEYIDEIEILAQNRSLEAYRLKSGKMGASMFXHXQALRXTLPFXPALSNPXXGX 510
            RYYGGNE+ID+ E L Q R+LEA+ L   K G ++  H       L    A+  P    
Sbjct: 72  ARYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPH-SGSPANLQAYQAVMKPHDRL 130

Query: 511 XGLNFPXGG 537
            GL+ P GG
Sbjct: 131 MGLDLPHGG 139


>SPAC24C9.12c |||glycine hydroxymethyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score =  101 bits (242), Expect = 1e-22
 Identities = 53/123 (43%), Positives = 71/123 (57%)
 Frame = +1

Query: 169 LWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGG 348
           L E DP + +I+  E DRQR+ + +IASENFTS  V+  L S + NKYSEG P  RYYGG
Sbjct: 12  LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71

Query: 349 NEYIDEIEILAQNRSLEAYRLKSGKMGASMFXHXQALRXTLPFXPALSNPXXGXXGLNFP 528
           N++ID+IE L Q R+L A+ L   K G ++          +    A+  P     GL+ P
Sbjct: 72  NKFIDQIETLCQERALAAFNLDPAKWGVNV-QCLSGSPANMQVYQAIMPPHGRLMGLDLP 130

Query: 529 XGG 537
            GG
Sbjct: 131 SGG 133


>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1072

 Score = 30.7 bits (66), Expect = 0.26
 Identities = 16/62 (25%), Positives = 33/62 (53%)
 Frame = +1

Query: 205  VKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQ 384
            V+E+D     L+M+  EN +   + +  ++CLH+   + +   R Y G++ +D + +L  
Sbjct: 957  VEERDPPSPLLQML--ENNSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRN 1014

Query: 385  NR 390
             R
Sbjct: 1015 KR 1016


>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 332

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
 Frame = +1

Query: 223 QRAGLEMIASENFTSVPVLQCLSSCLHNKY--SEGMPNQRYYGGNEYIDEIEIL 378
           Q+  L  I S   T    L CLS CL   Y    G+ +   + G E ++ +EIL
Sbjct: 192 QKLSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTS---FSGIEVLENLEIL 242


>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 510

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = -2

Query: 248 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLAL 144
           +I+ S +   S  LT + KSSG AS  + FSN +L
Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSL 431


>SPAC1952.16 |rga9||RhoGAp, GTPase activator towards
           Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 684

 Score = 25.8 bits (54), Expect = 7.3
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +2

Query: 32  NXLXFENTS*MLLVIDLLNIFINCRHSLRKDTLVP 136
           N +  E+T   LLVIDL+N F N    L + +  P
Sbjct: 612 NIIPVEDTHPTLLVIDLINEFENLFADLERPSTPP 646


>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1010

 Score = 25.4 bits (53), Expect = 9.6
 Identities = 15/58 (25%), Positives = 29/58 (50%)
 Frame = +1

Query: 142 MSAKLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYS 315
           ++ + LN  L E D   + +   +KD +  G  ++ +EN     +L  LS+  +N +S
Sbjct: 84  ITCQALNITLSETDSSKYYLEGFKKDLEEEGSPLLFNENNVDSALLSRLSTTGNNTFS 141


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,462,432
Number of Sequences: 5004
Number of extensions: 47261
Number of successful extensions: 114
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 394431430
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)