BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_J05
(933 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.004
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 33 0.076
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.94
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.0
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 37.1 bits (82), Expect = 0.004
Identities = 25/101 (24%), Positives = 28/101 (27%), Gaps = 2/101 (1%)
Frame = +1
Query: 607 NXXNNRXXPPXXPPPXKXPKXXXPXP--GGTKKTPLSPRXXPPXGXPPXAPPXSXXXAXP 780
N +N PP PPP P P G + P PR P G P S + P
Sbjct: 329 NGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNP 388
Query: 781 XPPXXXXPXEKXGXXXXXXKKGRKXXXKNXXXXQXPPXTPP 903
P P R PP PP
Sbjct: 389 PAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPP 429
Score = 35.1 bits (77), Expect = 0.014
Identities = 19/64 (29%), Positives = 25/64 (39%), Gaps = 2/64 (3%)
Frame = +1
Query: 619 NRXXPPXXPPPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXA-P-PXSXXXAXPXPPX 792
N+ PP PPP + + P G+ + L P PP + P P A P PP
Sbjct: 307 NKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366
Query: 793 XXXP 804
P
Sbjct: 367 RSAP 370
Score = 30.7 bits (66), Expect = 0.31
Identities = 24/89 (26%), Positives = 26/89 (29%)
Frame = +1
Query: 523 PGXXXAPHPXXRXKKXXXAGGPKXRXPENXXNNRXXPPXXPPPXKXPKXXXPXPGGTKKT 702
P AP P P +N PP P P P G +T
Sbjct: 355 PQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPAL--PPLGNASRT 412
Query: 703 PLSPRXXPPXGXPPXAPPXSXXXAXPXPP 789
P PP PP APP A P P
Sbjct: 413 STPPVPTPP-SLPPSAPPSLPPSAPPSLP 440
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/61 (31%), Positives = 20/61 (32%), Gaps = 3/61 (4%)
Frame = +1
Query: 631 PPXXPP---PXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPPXSXXXAXPXPPXXXX 801
PP PP P P P P +P P P APP A P PP
Sbjct: 424 PPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP--LPPAAPAPPPAPA 481
Query: 802 P 804
P
Sbjct: 482 P 482
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.7 bits (71), Expect = 0.076
Identities = 18/58 (31%), Positives = 19/58 (32%)
Frame = +1
Query: 646 PPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPPXSXXXAXPXPPXXXXPXEKXG 819
PP P P P P+ P G PP PP A P PP P G
Sbjct: 732 PPPPPPAVIVPTPAPAP-IPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAG 788
Score = 26.6 bits (56), Expect = 5.0
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = -2
Query: 527 PGPXXXPXGXPXPXXXXPPPRPP 459
P P P P P PPP PP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPP 766
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 0.94
Identities = 24/101 (23%), Positives = 27/101 (26%)
Frame = +1
Query: 631 PPXXPPPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPPXSXXXAXPXPPXXXXPXE 810
P PP P P P K + +P P G PP P P P P
Sbjct: 1126 PSVAAPPVPVPSGAPPVP---KPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVP 1182
Query: 811 KXGXXXXXXKKGRKXXXKNXXXXQXPPXTPPPXXKXGEXTP 933
K + PP PPP TP
Sbjct: 1183 KPAAGVPPVPPPSEAPPVPKPSVGVPP-VPPPSTAPPVPTP 1222
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/54 (29%), Positives = 16/54 (29%)
Frame = +1
Query: 643 PPPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPPXSXXXAXPXPPXXXXP 804
P P P P P SP PP PP AP P P P
Sbjct: 961 PRPAAPPSIPPPLP--VSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVP 1012
Score = 26.6 bits (56), Expect = 5.0
Identities = 15/53 (28%), Positives = 17/53 (32%), Gaps = 1/53 (1%)
Frame = +1
Query: 631 PPXXPPPXKXPKXXXPXPGGTKKTPL-SPRXXPPXGXPPXAPPXSXXXAXPXP 786
P PP P P P T P+ P PP P S + P P
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAP 1079
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/42 (33%), Positives = 14/42 (33%)
Frame = +1
Query: 631 PPXXPPPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPP 756
PP P P P P G P PP P APP
Sbjct: 1102 PPVPKPSVAVPPV--PAPSGAPPVPKPSVAAPPVPVPSGAPP 1141
Score = 25.8 bits (54), Expect = 8.7
Identities = 14/42 (33%), Positives = 14/42 (33%)
Frame = +1
Query: 631 PPXXPPPXKXPKXXXPXPGGTKKTPLSPRXXPPXGXPPXAPP 756
PP P P P P G P PP P APP
Sbjct: 1121 PPVPKPSVAAPPV--PVPSGAPPVPKPSVAAPPVPAPSGAPP 1160
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.2
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = +2
Query: 728 PXGXPRXPPPXXXTKPXPXPPPKXXP 805
P G P PPP +P PPP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 725 PPXGXPRXPPPXXXTKPXPXPPP 793
PP P PPP P PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPP 27
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.0
Identities = 14/43 (32%), Positives = 15/43 (34%), Gaps = 1/43 (2%)
Frame = +1
Query: 631 PPXXPPPXKXPKXXXP-XPGGTKKTPLSPRXXPPXGXPPXAPP 756
PP P P+ P P P P P PP APP
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.309 0.143 0.482
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,516,074
Number of Sequences: 5004
Number of extensions: 17336
Number of successful extensions: 88
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 43 (22.0 bits)
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