Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_H15
(897 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 242 5e-65
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 134 1e-32
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 132 5e-32
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 89 6e-19
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 71 3e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 69 1e-12
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 62 8e-11
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 58 2e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 50 4e-07
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 34 0.024
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.22
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.8
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 242 bits (592), Expect = 5e-65
Identities = 105/117 (89%), Positives = 114/117 (97%)
Frame = +3
Query: 123 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 302
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 303 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREE 473
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NRE+
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREK 118
Score = 210 bits (513), Expect = 2e-55
Identities = 99/108 (91%), Positives = 106/108 (98%)
Frame = +1
Query: 469 KKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 648
+KMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+
Sbjct: 117 EKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIM 176
Query: 649 RLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE 792
RLDLAGRDLTDYLMKIL ERGY+F+TTAEREIVRDIKEKLCYVALDFE
Sbjct: 177 RLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFE 224
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/18 (88%), Positives = 17/18 (94%)
Frame = +3
Query: 825 LEKSYELPDGQVITIGTK 878
LEKSYELPDGQVITIG +
Sbjct: 236 LEKSYELPDGQVITIGNE 253
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 134 bits (325), Expect = 1e-32
Identities = 61/108 (56%), Positives = 81/108 (75%)
Frame = +1
Query: 469 KKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 648
+KM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H +
Sbjct: 117 EKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVG 176
Query: 649 RLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFE 792
RLD+AGRD T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E
Sbjct: 177 RLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLE 224
Score = 118 bits (283), Expect = 1e-27
Identities = 54/115 (46%), Positives = 81/115 (70%), Gaps = 2/115 (1%)
Frame = +3
Query: 135 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 308
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 309 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREE 473
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANRE+
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREK 118
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 132 bits (320), Expect = 5e-32
Identities = 61/116 (52%), Positives = 81/116 (69%), Gaps = 1/116 (0%)
Frame = +3
Query: 129 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 308
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 309 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREE 473
+L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+E+
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEK 123
Score = 130 bits (314), Expect = 3e-31
Identities = 57/107 (53%), Positives = 83/107 (77%)
Frame = +1
Query: 469 KKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 648
+K+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQ 181
Query: 649 RLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 789
+ ++ GRD+TDYL L + G+ ++AE+EIVR+IKEK CYVA DF
Sbjct: 182 QEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDF 228
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 804 HRCRLHFLEKSYELPDGQVITIGTK 878
H+ ++H +Y+LPD Q IT+GT+
Sbjct: 238 HKPQIH----TYQLPDNQTITLGTE 258
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 89.4 bits (212), Expect = 6e-19
Identities = 52/134 (38%), Positives = 70/134 (52%), Gaps = 24/134 (17%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 269
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 270 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 428
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 429 LLTEAPLNPKANRE 470
LLTE PLNP NRE
Sbjct: 127 LLTEPPLNPPENRE 140
Score = 86.6 bits (205), Expect = 4e-18
Identities = 50/112 (44%), Positives = 70/112 (62%), Gaps = 10/112 (8%)
Frame = +1
Query: 481 QIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALP 636
+IMFE+FN +Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY +
Sbjct: 144 EIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIG 203
Query: 637 HAILRLDLAGRDLTDYLMKILTERGY--SFTTTAEREIVRDIKEKLCYVALD 786
+I + LAGRD+T ++ +L +R S TAER IKE+ CYV D
Sbjct: 204 SSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAER-----IKEECCYVCPD 250
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 70.5 bits (165), Expect = 3e-13
Identities = 39/114 (34%), Positives = 61/114 (53%)
Frame = +3
Query: 126 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 305
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 306 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 467
G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR 114
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/81 (29%), Positives = 40/81 (49%)
Frame = +1
Query: 472 KMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 651
K + MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 116 KTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQV 175
Query: 652 LDLAGRDLTDYLMKILTERGY 714
+G + D L + L ++ +
Sbjct: 176 QHFSGNAINDILAQTLRDKNF 196
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 68.5 bits (160), Expect = 1e-12
Identities = 42/132 (31%), Positives = 74/132 (56%), Gaps = 1/132 (0%)
Frame = +3
Query: 63 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 242
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R +
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60
Query: 243 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 419
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + E
Sbjct: 61 ----KLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115
Query: 420 HPVLLTEAPLNP 455
HP+ +TE NP
Sbjct: 116 HPICMTEPLANP 127
Score = 54.8 bits (126), Expect = 2e-08
Identities = 28/107 (26%), Positives = 54/107 (50%), Gaps = 2/107 (1%)
Frame = +1
Query: 475 MTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAIL 648
MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +P+ G +
Sbjct: 134 MTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAK 193
Query: 649 RLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF 789
R+ G + YL+K+ + SF + C+V+ D+
Sbjct: 194 RISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCHVSSDY 240
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 62.5 bits (145), Expect = 8e-11
Identities = 34/118 (28%), Positives = 64/118 (54%), Gaps = 2/118 (1%)
Frame = +3
Query: 126 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 305
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 306 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREE 473
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQ 119
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 58.0 bits (134), Expect = 2e-09
Identities = 27/77 (35%), Positives = 48/77 (62%)
Frame = +1
Query: 571 IVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVR 750
+V+DSG +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV
Sbjct: 159 LVIDSGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVN 216
Query: 751 DIKEKLCYVALDFEXXM 801
+IKE +CYV+ +F+ M
Sbjct: 217 EIKESVCYVSQNFKEDM 233
Score = 28.7 bits (61), Expect = 1.2
Identities = 26/104 (25%), Positives = 47/104 (45%), Gaps = 4/104 (3%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 320
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 321 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTE 440
++ H G + +W +W N + P ++ +LLT+
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQ 101
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 50.0 bits (114), Expect = 4e-07
Identities = 29/95 (30%), Positives = 46/95 (48%)
Frame = +1
Query: 478 TQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLD 657
TQ FE P +A + ++ LYA G G+V+D G + PI +G + A +L
Sbjct: 166 TQFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLP 225
Query: 658 LAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKE 762
L GR +T +L +L E + ++ + DI E
Sbjct: 226 LGGRYMTQHLQNLLRESLPTLKSSGQYVSKEDITE 260
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 34.3 bits (75), Expect = 0.024
Identities = 16/84 (19%), Positives = 42/84 (50%)
Frame = +1
Query: 469 KKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAIL 648
+K+ I+F + + +++ + + +G + V+D G + + EG +P++ +
Sbjct: 260 EKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNSRI 319
Query: 649 RLDLAGRDLTDYLMKILTERGYSF 720
+++ G D+T MK+L + +
Sbjct: 320 KINYGGDDITLLFMKLLMRSHFPY 343
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.22
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +3
Query: 186 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 329
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.8
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 614 STKVTLCPTPSSVWTW 661
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,283,166
Number of Sequences: 5004
Number of extensions: 65563
Number of successful extensions: 239
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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