Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_G17
(893 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 122 7e-29
SPAP8A3.10 |||mitochondrial intermembrane space protein sorting ... 71 2e-13
SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr... 27 2.7
SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.7
SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces pom... 27 2.7
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 27 3.6
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 27 3.6
SPAC22E12.14c |sck2||serine/threonine protein kinase Sck2|Schizo... 27 4.8
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.3
SPBC1289.13c |||alpha-1,2-galactosyltransferase|Schizosaccharomy... 26 8.3
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 26 8.3
SPAC21E11.07 ||SPAC2C4.01|glycine cleavage T-protein|Schizosacch... 26 8.3
SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomy... 26 8.3
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 122 bits (294), Expect = 7e-29
Identities = 68/172 (39%), Positives = 99/172 (57%), Gaps = 3/172 (1%)
Frame = +1
Query: 187 MKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVD-GVLHTHRLVSSKWF 363
MKI+ S H F +P+E V+ A W+KYPN VI D ++RKV+D GVL+T RL++
Sbjct: 1 MKIFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKVLDNGVLYTERLITCHQA 60
Query: 364 FPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVDETVRYTPHPSDSSK 543
PRW LI A+ CY E S V+ R +TL T+NLTF + VDETV Y+PHP +
Sbjct: 61 LPRWILKLIDGAQDCYIRETSYVDLKARTLTLLTSNLTFSDRLRVDETVTYSPHP-ELEA 119
Query: 544 TLLKQEAVVTVQGV--PLSSYMEDLLTNKISLNAGKGRQAIEWVIGKLTLKL 693
T+ +QEA + LS+ +E + A +G++ E V+ K+ + +
Sbjct: 120 TVFQQEARIEALACMKRLSNLIEQWSVDGFGKKASRGKEGFESVLEKINMSV 171
>SPAP8A3.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 171
Score = 70.9 bits (166), Expect = 2e-13
Identities = 42/121 (34%), Positives = 64/121 (52%), Gaps = 1/121 (0%)
Frame = +1
Query: 193 IWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVD-GVLHTHRLVSSKWFFP 369
I T + N W TV+ A +YPNP + V+ DV+ER V D G L+T RL+ + P
Sbjct: 4 ICTDKTELNASWNTVSSAWLTRYPNPYSLHVVSADVLERYVDDEGRLYTERLLVKQGRLP 63
Query: 370 RWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHYIAVDETVRYTPHPSDSSKTL 549
RWA L+ K Y E S ++P ++++ +T NL + V E R+ + SKT+
Sbjct: 64 RWASDLLNVNK-SYILERSVIDPSKQELKSETFNLDHVKILRVIEYSRFIQSSENCSKTI 122
Query: 550 L 552
+
Sbjct: 123 V 123
>SPBC21.02 |||TLDc domain protein 2|Schizosaccharomyces pombe|chr
2|||Manual
Length = 511
Score = 27.5 bits (58), Expect = 2.7
Identities = 26/117 (22%), Positives = 47/117 (40%)
Frame = +1
Query: 139 RSLSLYCNLH*ILEKNMKIWTSEHTFNHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVV 318
R + Y N +L K KI + + + P A RKYP P IGTD +
Sbjct: 316 RKMCNYHNPSILLIKAKKINANHKSSSRPISLDATIP-RKYP----PHCIGTDKAVPQKF 370
Query: 319 DGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTNLTFCHY 489
H ++ + RW Q+ +G +++ + ++ PI + + +C +
Sbjct: 371 GADFHNENILLGAYISTRWRQSHMGFFG-DHSTLLFQLQPIHQVYYASNLDKNYCMF 426
>SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 525
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/62 (25%), Positives = 26/62 (41%)
Frame = +1
Query: 220 HPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVDGVLHTHRLVSSKWFFPRWAQALIGTA 399
H W+ + YPN AV ++ G +H L+ S+ WA A++G A
Sbjct: 431 HIWQAMLLYVTDAYPNVSASAVAAFELPSNLGAVGFIHLSALMFSR-MNVHWATAVVGFA 489
Query: 400 KI 405
+
Sbjct: 490 SL 491
>SPBC19G7.07c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 687
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/41 (31%), Positives = 25/41 (60%)
Frame = +3
Query: 630 SKCWQRKASYRMGYREVDTEIKELASSACKVQMNYYHNKKS 752
++C +AS+++ +E+D +I+E V++N NKKS
Sbjct: 47 ARCSTNEASWKLAQKELDLKIREYEQKLKDVKLNDI-NKKS 86
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 505 FHQQQCN-DRMLGLLFLKSSVAVLGLLLISH*HN 407
FH Q N D++L + FL V ++G+L +H HN
Sbjct: 443 FHPPQMNTDQLLLVSFLGLVVNLVGILAFNHGHN 476
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/24 (54%), Positives = 16/24 (66%)
Frame = +3
Query: 744 KKSIDDITTSARRSMDDISSKAKS 815
KKS DDITT +R M+D S +S
Sbjct: 181 KKSKDDITTPKKRLMEDTYSPRES 204
>SPAC22E12.14c |sck2||serine/threonine protein kinase
Sck2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 646
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/41 (29%), Positives = 20/41 (48%)
Frame = +1
Query: 217 NHPWETVAQAAWRKYPNPMNPAVIGTDVVERKVVDGVLHTH 339
+HP T A PNP+ P+ + DV + +D ++ H
Sbjct: 165 SHPIPTPAIQRTSSIPNPLTPSYVVFDVAKPVPIDVNIYDH 205
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 705 SSACKVQMNYYHNKKSIDDITTSARRSMDDISSK 806
SS+ V +YHN S + S+ S+ +SSK
Sbjct: 414 SSSAPVSSAFYHNSTSSRSSSHSSSHSLSSLSSK 447
>SPBC1289.13c |||alpha-1,2-galactosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 375
Score = 25.8 bits (54), Expect = 8.3
Identities = 14/55 (25%), Positives = 22/55 (40%)
Frame = +1
Query: 460 KTTNLTFCHYIAVDETVRYTPHPSDSSKTLLKQEAVVTVQGVPLSSYMEDLLTNK 624
+TT +T E PHP +S LL PL+ Y+ ++ N+
Sbjct: 77 ETTTVTPTVTATAQELSTLNPHPENSKIVLLMGSNAQNDPSSPLNPYIRTIIKNR 131
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/55 (23%), Positives = 29/55 (52%)
Frame = +1
Query: 307 RKVVDGVLHTHRLVSSKWFFPRWAQALIGTAKICYASEISEVNPIQRQMTLKTTN 471
RK+++ +LH+H +SS +F+ + + A + + +NP R + ++N
Sbjct: 42 RKIINEMLHSHSELSS-FFYAEKSNTFLTIAGLAESELFDTLNPAFRISSCPSSN 95
>SPAC21E11.07 ||SPAC2C4.01|glycine cleavage
T-protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 325
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = +1
Query: 538 SKTLLKQEAVVTVQGVPLSSYMEDLLTNKISLN 636
S L ++++ V+GV +++ L TNKI+L+
Sbjct: 22 SVCLNSSKSLIRVEGVDAVKFLQGLTTNKITLD 54
>SPAC3A12.15 |vps53||GARP complex subunit Vps53 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 756
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +3
Query: 741 NKKSIDDITTSARRSMDDISSKAKSXLDDXXKXTKANANXRS 866
NKK+ +D S MDD++S S L + N +S
Sbjct: 59 NKKTYEDAKLSMGEKMDDLNSSIVSLLQELSTLQSVAENTQS 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,476,430
Number of Sequences: 5004
Number of extensions: 71948
Number of successful extensions: 180
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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