Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP21_F_D24
         (933 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ...   256   3e-69
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd...   239   4e-64
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha...    58   1e-09
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar...    29   0.71 
SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy...    29   0.71 
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    29   0.94 
SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch...    27   3.8  
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr...    27   3.8  
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|...    27   5.0  
SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|ch...    27   5.0  
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc...    26   6.6  
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom...    26   6.6  
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch...    26   8.7  
SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr 1||...    26   8.7  
SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase |Schizosacch...    26   8.7  

>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 937

 Score =  256 bits (627), Expect = 3e-69
 Identities = 125/259 (48%), Positives = 177/259 (68%), Gaps = 1/259 (0%)
 Frame = +3

Query: 117 GIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAE 296
           G  +A  +  +LR+Q++ ++S    F   L I+QVGGREDSNVY+RMK +AA + GI+ E
Sbjct: 7   GTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANEAGISCE 66

Query: 297 HIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLN 476
           H+  P DITE +LL  I   NE P+VHGIIVQ+PL +   I+   +T+AV+P+KDVDG  
Sbjct: 67  HVNFPEDITEYDLLLAIKGFNEDPTVHGIIVQLPLPAH--INEQIITEAVAPEKDVDGFC 124

Query: 477 TINEGRVAVGDLSG-FIPCTPAGCVELIKKTGVTIAGKNVVVLGRSRIVGTPVSELLKWE 653
             N G++   +    F  CTP G + ++K  G+ + GK+ VV+GRS IVG P+S LL+  
Sbjct: 125 ETNLGKLTKREGQPLFTACTPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKA 184

Query: 654 HATVTVCHSKTKNLSEITKTAEILVVAIGRPEXVRGSWIKPGAVVIDCGINAIEDPTKKS 833
           +ATVT+CHSKT+++++I +TA+I+V AIG P  V+  W+K G V ID GIN+I D TKKS
Sbjct: 185 NATVTLCHSKTESIADIVRTADIVVAAIGIPHFVKADWLKKGVVAIDVGINSIPDATKKS 244

Query: 834 GQRLXGDVAYQXAVKVXSA 890
           G RL GD+ ++ A +V SA
Sbjct: 245 GYRLTGDIDFENAKEVASA 263


>SPBC2G2.08 |ade9||C-1-
           tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
           ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
           trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 969

 Score =  239 bits (585), Expect = 4e-64
 Identities = 131/293 (44%), Positives = 189/293 (64%), Gaps = 6/293 (2%)
 Frame = +3

Query: 30  NSLRFKLLESGLDRPTR---QSSGLRWHKLYL-GIEVAGSIENDLRQQVTRLRSKWSGFE 197
           N LR   LES   RP++   QS G       L G  +A  ++  + ++V  L++K   F+
Sbjct: 5   NQLRNYFLESNSLRPSKWLFQSYGTSSSANILNGKLLARKLQRSVAEEVQALKAKDRNFK 64

Query: 198 PRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKITSLNESPSVH 377
           P LAIVQVG REDSNVY+RMK KAA  +GI  ++   P  I    LL ++  LN+  +VH
Sbjct: 65  PALAIVQVGKREDSNVYVRMKEKAARLVGIDFKYCPFPETIQMPALLHELKKLNDDHTVH 124

Query: 378 GIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTINEGRVAVGDLSGF-IPCTPAGCVEL 554
           G++VQ+PL     ++   VT++++P KDVDG    N G +A  D +    PCTP G +EL
Sbjct: 125 GVLVQLPLPKH--LNERTVTESITPPKDVDGFGAFNIGLLAKNDATPIHYPCTPKGIMEL 182

Query: 555 IKKTGVTIAGKNVVVLGRSRIVGTPVSELLKWEHATVTVCHSKTKNLSEITKTAEILVVA 734
           +K   +++AG N VVLGRS IVG P+S LL+ ++ATVTVCHSKTK+L +    A++++ A
Sbjct: 183 LKDNKISVAGLNAVVLGRSDIVGNPISYLLRKDNATVTVCHSKTKDLIQHISNADLVIAA 242

Query: 735 IGRPEXVRGSWIKPGAVVIDCGINAIEDPTKKSGQR-LXGDVAYQXAVKVXSA 890
           +G+PE VRG W+KPG+VV+D GINA++    ++G+R L GDV ++ A KV S+
Sbjct: 243 LGKPEFVRGEWLKPGSVVVDVGINAVQ----RNGKRVLVGDVHFESASKVASS 291


>SPBC1711.04 |||methylenetetrahydrofolate reductase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 320

 Score = 58.4 bits (135), Expect = 1e-09
 Identities = 57/204 (27%), Positives = 90/204 (44%), Gaps = 17/204 (8%)
 Frame = +3

Query: 105 KLYLGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIG 284
           K+     VA +    L+Q V         F P+L          + +Y     K   +IG
Sbjct: 14  KVVYASRVAETFVEQLKQHVNLFE-----FAPKLVGFLSNSDPAARMYADWTNKTCTEIG 68

Query: 285 IAAEHIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDV 464
              E   +P+D    +L   I   N  PSV+GI++  P+ +D   D + +   VSPDKDV
Sbjct: 69  FQYELREVPKD----DLEDAIVEANNDPSVNGIMIYFPVFNDGQ-DQY-LQQVVSPDKDV 122

Query: 465 DGL------NTINEGRVAVGDLS--GFIPCTPAGCVELIKKTGV---------TIAGKNV 593
           +GL      N  +  R    + +    +PCTP   V++++  GV          + GK +
Sbjct: 123 EGLCHKYVMNMYHNIRHLDPEKTKKSILPCTPLAIVKILEYLGVYNKIINYGNRLYGKTI 182

Query: 594 VVLGRSRIVGTPVSELLKWEHATV 665
            ++ RS IVG P++ LL  + A V
Sbjct: 183 TIVNRSEIVGRPLAALLANDGAKV 206


>SPBC23G7.08c |rga7||GTPase activating protein
           Rga7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 29.5 bits (63), Expect = 0.71
 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
 Frame = -3

Query: 622 PTMRLRPSTTTFL-PAIVTPVFLMSSTHPAGVQGINPDRSPTATRPSLIVFKPSTS 458
           P     P+ +TF  P++ +P F  SST        NP  +P +  P     KPST+
Sbjct: 366 PLQNTNPAPSTFPNPSVASPAFPNSSTS-------NPSTAPASASPLASTLKPSTA 414


>SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 537

 Score = 29.5 bits (63), Expect = 0.71
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = -3

Query: 148 SFSIDPATSIPRYNLCHLSPEDCLVGRSNPDSNNLNLKEFPI 23
           S +I+     P  +L    PE  LV +S  D NN N+ EFP+
Sbjct: 18  STTINKPNVHPSMSLLKALPECRLVVKSKGDKNNANVVEFPL 59


>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 29.1 bits (62), Expect = 0.94
 Identities = 29/109 (26%), Positives = 48/109 (44%), Gaps = 1/109 (0%)
 Frame = -3

Query: 760 PRTISGRPIAT-TSISAVLVISLKFLVFEWHTVTVACSHFSNSDTGVPTMRLRPSTTTFL 584
           P T + R   T TS +++   S           + + S +SN  T   T+   P TT+F 
Sbjct: 640 PLTSTNRTSTTFTSSTSISTSSSSTATSSTSFASESSSFYSNVTTSSSTVSTPPPTTSF- 698

Query: 583 PAIVTPVFLMSSTHPAGVQGINPDRSPTATRPSLIVFKPSTSLSGETAS 437
           P+  T  F+ SS+  +     N     TA+  S    K +++ SG ++S
Sbjct: 699 PSTFTTSFITSSSLSSIPN--NSTEVKTASTSSGTEIKTASTSSGSSSS 745


>SPAC14C4.08 |mug5||meiotically upregulated gene
           Mug5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 179

 Score = 27.1 bits (57), Expect = 3.8
 Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
 Frame = +3

Query: 186 SGFEPRLAIVQVGGRE-DSNVYIRMKLKAAEKI 281
           SGFE R+ I++    E DSN+YIR+K +   K+
Sbjct: 11  SGFENRIKILEDLMNEFDSNLYIRLKRECDFKL 43


>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 512

 Score = 27.1 bits (57), Expect = 3.8
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -2

Query: 401 KGHLNNDAVNRRRFIQTRYF 342
           KG+LNN A N+  F + R+F
Sbjct: 365 KGYLNNPAANKSSFTKDRFF 384


>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 927

 Score = 26.6 bits (56), Expect = 5.0
 Identities = 23/95 (24%), Positives = 40/95 (42%), Gaps = 8/95 (8%)
 Frame = -2

Query: 446 DSISDSMSVDRVVRIKGHLNNDAVNR------RRFIQTRYFC*QLYFSDVPW*--ADMFG 291
           D+ S +     +VR++   N + V        R   +  YFC   +     W   A  F 
Sbjct: 701 DASSSAAFNASMVRLRSTFNEELVELFENMAVRSLNRAEYFCTGDFGEKTDWHHYALSFN 760

Query: 290 GDTNFLSCLQLHPDVDVRVLAPAHLHDGKPGLEAR 186
             T+F S ++ +PD+ V  L    L +  PG++ +
Sbjct: 761 HYTHFTSPIRRYPDIIVHRLLERSLKNTSPGIDKK 795


>SPBC25H2.11c |||bromodomain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 979

 Score = 26.6 bits (56), Expect = 5.0
 Identities = 9/23 (39%), Positives = 13/23 (56%)
 Frame = +2

Query: 62  IGSTNETVLWAKMAQIISWD*SC 130
           I   N+T+LW +   +I WD  C
Sbjct: 42  ISILNDTLLWNRFINVIEWDKLC 64


>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
           Sap155|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1188

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 13/28 (46%), Positives = 18/28 (64%)
 Frame = +1

Query: 760 ALGSSLEPSL*TVVSTLLRILQRKAGNV 843
           ALG+  +P L  +VST+L  L  K+ NV
Sbjct: 804 ALGTRCKPYLPQIVSTILYRLNNKSANV 831


>SPBC1A4.07c |||U3 snoRNP-associated protein
           Sof1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 436

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 13/51 (25%), Positives = 24/51 (47%)
 Frame = +3

Query: 150 LRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHI 302
           L + +T+LR+    + P  A   V G ED N+Y+         + +  +H+
Sbjct: 223 LTKLITKLRTNSISWNPMEAFNFVAGSEDHNLYMYDMRNLKRALHVYKDHV 273


>SPBC1289.02c |uap2||U2 snRNP-associated protein
           Uap2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 367

 Score = 25.8 bits (54), Expect = 8.7
 Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
 Frame = +3

Query: 324 EIELLAKITSLNESPSVHGIIVQMPLD-SDHAIDAHRVTDAVSPDKDVDGLNTI 482
           +I +L  I +L E      +++ +  D ++ A    RVT+ V  DK+ DG+ T+
Sbjct: 249 KIVVLKHIFTLEELDKTPELLIDLKDDITEEAEKCGRVTNVVLYDKEPDGVVTV 302


>SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 247

 Score = 25.8 bits (54), Expect = 8.7
 Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
 Frame = +3

Query: 123 EVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRM--KLKAAEK 278
           E+  S++ D+RQ++ +L  + S  E    I +   RE     IRM  +L++AE+
Sbjct: 31  EIESSLK-DVRQELQKLNEEQSRLEKNAQIPEYRVRESEAFLIRMQRRLESAEE 83


>SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 25.8 bits (54), Expect = 8.7
 Identities = 16/34 (47%), Positives = 20/34 (58%)
 Frame = +3

Query: 246 YIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKI 347
           YI  KL  A   G+A + I    DI E+E+LAKI
Sbjct: 23  YISPKLYIASS-GVAGDGIFSTFDIDELEVLAKI 55


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,587,423
Number of Sequences: 5004
Number of extensions: 72973
Number of successful extensions: 184
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)