Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_B15
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 65 1e-11
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 46 9e-06
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.094
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 29 0.88
SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-tr... 28 2.0
SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 3.5
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.5
SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |S... 26 6.2
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 8.2
SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schiz... 26 8.2
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 8.2
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 64.9 bits (151), Expect = 1e-11
Identities = 37/122 (30%), Positives = 66/122 (54%)
Frame = +3
Query: 429 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEK 608
N+I F EL + C + + AE A +GP+ + LA KY + I+ E++EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 609 HSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFAXRYGKIAVNI 788
S+I++N+ + I++ GN+ G +RK H+ F+ + + + P+F +GK+ V I
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIFETSFGKLGVMI 149
Query: 789 CF 794
C+
Sbjct: 150 CW 151
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 45.6 bits (103), Expect = 9e-06
Identities = 49/185 (26%), Positives = 80/185 (43%), Gaps = 5/185 (2%)
Frame = +3
Query: 300 RIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCT 479
R ++G+VQ +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 42 RAFRIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--T 91
Query: 480 REKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGN 659
+ E E E P+ L +A + + E+ L+NTA+V +G
Sbjct: 92 GYFNQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGK 147
Query: 660 VIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFAXRYGKIAVNICFGRHHVLNWMM 824
+I HRK H IP F ES+ G+ + YGK + IC+ M+
Sbjct: 148 LIAVHRKIHLFDIDIPGGVSFRESDSLSPGD-AMTMVDTEYGKFGLGICYDIRFPELAMI 206
Query: 825 FGQNG 839
+NG
Sbjct: 207 AARNG 211
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.094
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -1
Query: 541 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 362
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 361 LTGR 350
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 29.1 bits (62), Expect = 0.88
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = +3
Query: 513 SAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDIL 623
SAE+ + + + +++VS++LE DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPBC28F2.03 |ppi1|cyp2|cyclophilin family peptidyl-prolyl cis-trans
isomerase Cyp2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 162
Score = 27.9 bits (59), Expect = 2.0
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -3
Query: 809 DVVPSEADVHRDLAVSXRKYRMAGVTFHVVV 717
DVVP A R L + Y AG TFH V+
Sbjct: 25 DVVPKTAANFRALCTGEKGYGYAGSTFHRVI 55
>SPAC17H9.01 |cid16||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1202
Score = 27.5 bits (58), Expect = 2.7
Identities = 29/116 (25%), Positives = 48/116 (41%)
Frame = -3
Query: 494 RLLLPCAEREGHVPQLLETDDVNTLLAGNIDDFLDFIENCFLLLVDWTIGGHRDGMLNYS 315
R LL C +R P +L DV ++DD + F+ T GH++ + N S
Sbjct: 119 RALLSCCKRSKD-PSILFPTDVPC----SLDDDVSFL----------TFKGHKNHLENRS 163
Query: 314 YLHNSRGSGLLVLGRESVCGDVEVSLLSCSDRGFFQFDFKVIPPPKMNSVELFQVA 147
+ H+S V+ E + L DR K++ P N ++L +V+
Sbjct: 164 FFHDSESDNFKVVLSNCAINSKEDNNLVTEDR--VNLGAKLLLVPVQNLIKLLKVS 217
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.1 bits (57), Expect = 3.5
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -3
Query: 392 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNSRGSGLLVLGRESVCGDVEVSL 237
+FI+ CF + + GH D +++ S +S GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 817 QFKTWCRPKQMFTAILPYLXANTGWPVLPS 728
+F T K +FTA+ P+L A T + ++PS
Sbjct: 1521 RFPTRVLCKPVFTAVPPFLFAGTDFALIPS 1550
>SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 492
Score = 26.2 bits (55), Expect = 6.2
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -1
Query: 757 ANTGWPVLPSM**LDSLKSPTLGMWFLR-CFPITFPVSLITTAVFQSMSECFSSLSN 590
A+ G P++ + LD+ G W + + PVSLIT AVF + C SSL +
Sbjct: 247 ADDGKPLVEKI--LDAAGQKGTGKWTAQNALEMGTPVSLITEAVF---ARCLSSLKS 298
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 132 NNLTGRDLEEFNRIHFGRRNNLEI 203
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -3
Query: 782 HRDLAVSXRKYRMAGVTFHVVVGFVKIAD 696
HR+L V K A V H VVG K D
Sbjct: 202 HRELTVRAAKQHGARVLIHPVVGMTKPGD 230
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +3
Query: 102 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 212
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,545,126
Number of Sequences: 5004
Number of extensions: 74554
Number of successful extensions: 245
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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