Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP21_F_A02
(911 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein L23|Schizosac... 159 7e-40
SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein L23|Schizosacc... 159 7e-40
SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit L38|... 47 4e-06
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 6.5
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 26 6.5
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 26 8.5
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 8.5
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 26 8.5
>SPCC1322.11 |rpl2302|rpl23-2|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 159 bits (385), Expect = 7e-40
Identities = 70/84 (83%), Positives = 80/84 (95%)
Frame = +3
Query: 222 RLNRLPAAGSGDMIVATVKKGKPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVN 401
RLNRLPAA GDM++ATVKKGKP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN
Sbjct: 47 RLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVN 106
Query: 402 NKGEMKGSAITGPVAKECADLWPR 473
KGEMKGSAITGPVAKECADLWPR
Sbjct: 107 PKGEMKGSAITGPVAKECADLWPR 130
Score = 30.3 bits (65), Expect = 0.40
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +2
Query: 104 AGAKFRISLGSPXWEQ*STAPTTQGAKNLYVIAVQG 211
+G K+R++LG P + A + GAKNLY+++V G
Sbjct: 9 SGTKYRMTLGLPVQAIMNCADNS-GAKNLYIVSVFG 43
>SPAC3G9.03 |rpl2301|rpl23-1|60S ribosomal protein
L23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 139
Score = 159 bits (385), Expect = 7e-40
Identities = 70/84 (83%), Positives = 80/84 (95%)
Frame = +3
Query: 222 RLNRLPAAGSGDMIVATVKKGKPELRKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVIVN 401
RLNRLPAA GDM++ATVKKGKP+LRKK+MPA+V+RQRK +RR+DGV++YFEDNAGVIVN
Sbjct: 47 RLNRLPAASCGDMVLATVKKGKPDLRKKIMPAIVVRQRKAWRRKDGVYLYFEDNAGVIVN 106
Query: 402 NKGEMKGSAITGPVAKECADLWPR 473
KGEMKGSAITGPVAKECADLWPR
Sbjct: 107 PKGEMKGSAITGPVAKECADLWPR 130
Score = 30.3 bits (65), Expect = 0.40
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = +2
Query: 104 AGAKFRISLGSPXWEQ*STAPTTQGAKNLYVIAVQG 211
+G K+R++LG P + A + GAKNLY+++V G
Sbjct: 9 SGTKYRMTLGLPVQAIMNCADNS-GAKNLYIVSVFG 43
>SPBC887.07 |mrpl38||mitochondrial ribosomal protein subunit
L38|Schizosaccharomyces pombe|chr 2|||Manual
Length = 126
Score = 46.8 bits (106), Expect = 4e-06
Identities = 28/79 (35%), Positives = 41/79 (51%), Gaps = 9/79 (11%)
Frame = +3
Query: 243 AGSGDMIVATVKKGKPEL---------RKKVMPAVVIRQRKPFRRRDGVFIYFEDNAGVI 395
A GD +V VKK + R + A+++R + P RR DG ++ F+DNA V+
Sbjct: 31 ASLGDEVVVVVKKARSGSSVTAANKVKRGDIHHAIIVRTKSPVRRPDGRYVRFDDNACVL 90
Query: 396 VNNKGEMKGSAITGPVAKE 452
VN + E G+ I VA E
Sbjct: 91 VNKECEPLGTRILSVVANE 109
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 141 LGXPREIRNFAPAGTTTSSLRHFVVSK 61
L P+E NF AG T+ L++++VSK
Sbjct: 1344 LWRPKEEINFQEAGVLTAPLKNWLVSK 1370
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 26.2 bits (55), Expect = 6.5
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = +3
Query: 276 KKGKPELRKKVMPAVVIRQRK---PFRRRDGVFIYFEDNA 386
KKG P RK P+++ PF RD +FI E NA
Sbjct: 337 KKGAPVFRKH-FPSILQELNSLDPPFYERDAIFIVDEGNA 375
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.8 bits (54), Expect = 8.5
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = -2
Query: 424 EPFISPLLLTMTPALSSKYINTPS 353
+P+ P+L + P L Y++ PS
Sbjct: 275 QPYSGPILASTAPTLDGSYLSNPS 298
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 25.8 bits (54), Expect = 8.5
Identities = 12/51 (23%), Positives = 25/51 (49%)
Frame = -2
Query: 466 HRSAHSLATGPVMAEPFISPLLLTMTPALSSKYINTPSRLLNGFRCLMTTA 314
+ + H GP++ ++ L P +S Y+N + L+GF+ ++ A
Sbjct: 963 YNATHFEHIGPMLEATWMPILAALSNPLQNSDYVNELNMCLDGFQLVVRIA 1013
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.8 bits (54), Expect = 8.5
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = -2
Query: 472 RGHRSAHSLATGPVMAEPFISPLLL-TMTPALSSKYINTPSRLLNGF 335
R H+ AH+ AEP ++P++ + P+++ +TPS L F
Sbjct: 60 RRHKDAHAYDMSLTDAEPALAPVMSPAIPPSMNYPTPSTPSVLPESF 106
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,748,094
Number of Sequences: 5004
Number of extensions: 47292
Number of successful extensions: 103
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 97
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 462505890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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