Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_P21
         (897 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    55   1e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    44   4e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    43   5e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    36   0.008
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   3.6  
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe...    26   6.3  
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ...    26   8.4  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   8.4  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 55.2 bits (127), Expect = 1e-08
 Identities = 29/78 (37%), Positives = 43/78 (55%)
 Frame = +1

Query: 322 TAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCM 501
           T   RK K     E+  E  K++DK+ NG +   ELTH L +LGE+L   EVA++ ++  
Sbjct: 72  TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA- 130

Query: 502 DPEDDDGMIPYAAFLKKV 555
              D DG+I Y  F + +
Sbjct: 131 -DTDGDGVINYEEFSRVI 147



 Score = 29.1 bits (62), Expect = 0.90
 Identities = 15/61 (24%), Positives = 32/61 (52%)
 Frame = +1

Query: 364 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 543
           +F E   L+D++++G +   EL   + +LG+    +E+ ++  +     D +G I +  F
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70

Query: 544 L 546
           L
Sbjct: 71  L 71


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 43.6 bits (98), Expect = 4e-05
 Identities = 22/67 (32%), Positives = 41/67 (61%)
 Frame = +1

Query: 361 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 540
           E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K        DGM+ Y  
Sbjct: 77  EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYHD 133

Query: 541 FLKKVMA 561
           F++ ++A
Sbjct: 134 FVQMILA 140



 Score = 28.7 bits (61), Expect = 1.2
 Identities = 12/19 (63%), Positives = 15/19 (78%)
 Frame = +3

Query: 198 STLGDLLRALNSNPTLATI 254
           +++GDLLRA   NPTLA I
Sbjct: 26  TSIGDLLRACGQNPTLAEI 44


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 43.2 bits (97), Expect = 5e-05
 Identities = 21/75 (28%), Positives = 44/75 (58%)
 Frame = +1

Query: 337 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 516
           K +   + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP  +
Sbjct: 70  KLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TN 127

Query: 517 DGMIPYAAFLKKVMA 561
            G   Y  F++++MA
Sbjct: 128 SGSFDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.29
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = +1

Query: 361 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 540
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 35.9 bits (79), Expect = 0.008
 Identities = 21/74 (28%), Positives = 37/74 (50%)
 Frame = +1

Query: 274 RRRARSCSHSKSSFPSTAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 453
           +RR+R+ S + +     A  R   T    +D  E  KL+D +++  +   EL   + ALG
Sbjct: 8   KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67

Query: 454 EKLDDSEVAEVTKD 495
              + SEV ++ +D
Sbjct: 68  FNAEKSEVLKILRD 81



 Score = 27.9 bits (59), Expect = 2.1
 Identities = 19/74 (25%), Positives = 31/74 (41%)
 Frame = +1

Query: 337 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 516
           K   R   E+     +L+D +E G +    L      L E +DD E+  + ++     D 
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQ 159

Query: 517 DGMIPYAAFLKKVM 558
           DG I    F+  +M
Sbjct: 160 DGEINEQEFIAIMM 173


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +2

Query: 110 MSDLSKNDVER-ASFAFSIYDFEGKGKID 193
           +++L   DV R  SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633


>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 371

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +2

Query: 134 VERASFAFSIYDFEGKGKIDAFNLGRS 214
           +E A    SIYDF    +I+A N+G+S
Sbjct: 117 IEDAPPTQSIYDFSSSRQINALNVGQS 143


>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 474

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
 Frame = +1

Query: 274 RRRARSCSHSKSSFPSTAKQRKTKTRXAYE-DFLECLKLYDKNENGLM 414
           RR+  S SH  +SF   + +  +K R ++  D L+ L  + + E+G++
Sbjct: 36  RRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVV 83


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +1

Query: 448 LGEKLDDSEVAEVTKDCMDPED 513
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,049,883
Number of Sequences: 5004
Number of extensions: 53696
Number of successful extensions: 164
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)