Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_P14
(893 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 44 4e-05
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch... 37 0.004
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos... 28 2.1
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 2.1
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S... 26 6.3
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 6.3
SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase |Schizosaccharo... 26 6.3
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 26 8.3
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 26 8.3
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 43.6 bits (98), Expect = 4e-05
Identities = 42/148 (28%), Positives = 60/148 (40%)
Frame = +2
Query: 350 EGVVMELADCALPLLAGVLPTANPEEAFKDVAAAFLVGAMPRKEGMERKDLLAANVRIFK 529
EG M+L A PL + KD A + +K G R DLL AN+ IFK
Sbjct: 59 EGEAMDLNHAA-PLSHETRVYLGDYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFK 117
Query: 530 EQGQALDKAARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQLAAK 709
E + + K KD +LV NP + K + + T +D R Q +
Sbjct: 118 EILREVTKYT-KDAILLVATNPVDVLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKL 176
Query: 710 IGVPVKDVKRVIIWGNHSSTQFPDASNA 793
G+ + V II G H ++ S+A
Sbjct: 177 YGLDPQSVNADII-GEHGDSELAVWSHA 203
>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 509
Score = 36.7 bits (81), Expect = 0.004
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +2
Query: 542 ALDKAARKDVKVLVVGNPANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL 700
A+ + D K +VV + + A +CSKY PSIP +TR Q QS L
Sbjct: 394 AIGASIESDAKAIVVLSTSGNTARLCSKYRPSIP---IVMVTRCPQRARQSHL 443
>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 855
Score = 27.9 bits (59), Expect = 2.1
Identities = 24/62 (38%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
Frame = -3
Query: 483 PSFLGIAPTRKAAA--TSLKASSGFAVGRTPAKSGRAQSANSMTTPSSTPIIGAISRRWR 310
P F + P RK +A T+L SS P + RA SANS+T+P P A S + R
Sbjct: 516 PQFSAV-PHRKVSAQDTNLMGSSPGMYNHMPYLN-RATSANSITSPGVLPEGMAASLKKR 573
Query: 309 KT 304
T
Sbjct: 574 TT 575
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 27.9 bits (59), Expect = 2.1
Identities = 23/89 (25%), Positives = 37/89 (41%), Gaps = 5/89 (5%)
Frame = -3
Query: 588 PTTRTFTSLRAALSKAWPCSLKMRTLAARRSFLSIPSFLGIAPTRKAA-----ATSLKAS 424
P+ T + L + + P S T A S + S T ++ +T ++
Sbjct: 521 PSYNTSSVLPTSSVSSTPLSSANSTTATSASSTPLTSVNSTTATSASSTPFGNSTITSSA 580
Query: 423 SGFAVGRTPAKSGRAQSANSMTTPSSTPI 337
SG T SG + S+TTP+STP+
Sbjct: 581 SGSTGEFTNTNSGNGDVSGSVTTPTSTPL 609
>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
Klp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 26.2 bits (55), Expect = 6.3
Identities = 22/102 (21%), Positives = 45/102 (44%), Gaps = 2/102 (1%)
Frame = +2
Query: 500 LLAANVRIFKEQGQALDKAARKDVKV--LVVGNPANTNALICSKYAPSIPKENFTAMTRL 673
++ +R ++ + L + D+ V L + N + L+ + PK +
Sbjct: 153 IIFLTMRALLDRVEGLKRTMNVDISVSYLEIYNEKIRDLLVQDPLSMEKPK-SLNICEDA 211
Query: 674 DQNRAQSQLAAKIGVPVKDVKRVIIWGNHSSTQFPDASNAVA 799
+QN + L+ +++V +II GN + T P +NAV+
Sbjct: 212 EQNVSVPGLSYFTPTNLEEVMEIIIRGNSNRTMSPTEANAVS 253
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 26.2 bits (55), Expect = 6.3
Identities = 23/74 (31%), Positives = 26/74 (35%), Gaps = 9/74 (12%)
Frame = +2
Query: 593 PANTNALICSKYAPSIPKENFTAMTRLDQNRAQSQL---------AAKIGVPVKDVKRVI 745
P N+N S SIP T N AQS L A K+G K +
Sbjct: 240 PPNSNNANPSTLFSSIPSSRHTTSNHFPSNSAQSSLFSPTARPLTARKLGFASSQTKSAV 299
Query: 746 IWGNHSSTQFPDAS 787
NHS DAS
Sbjct: 300 S-NNHSRNSSKDAS 312
>SPAC13A11.06 ||SPAC3H8.01|pyruvate decarboxylase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 571
Score = 26.2 bits (55), Expect = 6.3
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = -3
Query: 642 GIDGAYLEHIRAFVLAGLPTTR 577
G+ G+Y EH+ + G+P+T+
Sbjct: 87 GVAGSYAEHVPVVHIVGMPSTK 108
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.8 bits (54), Expect = 8.3
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -3
Query: 768 VDEWFPQMITLLT 730
VDEWFP+M T LT
Sbjct: 231 VDEWFPKMETSLT 243
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 8.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -3
Query: 642 GIDGAYLEHIRAFVLAGLPTTRTFTS 565
GI GAY E++ +++G P T +S
Sbjct: 89 GIGGAYAENLPVILVSGSPNTNDLSS 114
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,531,955
Number of Sequences: 5004
Number of extensions: 71587
Number of successful extensions: 222
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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