Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_P10
(924 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 461 e-131
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 259 5e-70
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 250 2e-67
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 170 2e-43
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 121 2e-28
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 113 4e-26
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 100 3e-22
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 75 2e-14
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 68 2e-12
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 38 0.002
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.23
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.40
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.9
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 461 bits (1136), Expect = e-131
Identities = 212/238 (89%), Positives = 228/238 (95%)
Frame = +1
Query: 118 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 297
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 298 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQ 477
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 478 IMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLA 657
I+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+RLDLA
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLA 181
Query: 658 GRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEMATXAASTSLEKS 831
GRDLTDYLMKIL ERGY+F+TTAE EIVRDIKEKLCYVALDFE E+ T A S+SLEKS
Sbjct: 182 GRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAAQSSSLEKS 239
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/23 (65%), Positives = 15/23 (65%), Gaps = 1/23 (4%)
Frame = +3
Query: 831 YELPDGQVITSVRE-VPLSEALF 896
YELPDGQVIT E EALF
Sbjct: 240 YELPDGQVITIGNERFRAPEALF 262
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 259 bits (634), Expect = 5e-70
Identities = 119/225 (52%), Positives = 163/225 (72%), Gaps = 1/225 (0%)
Frame = +1
Query: 124 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRG 303
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 304 ILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEAPLNPKANREKMTQI 480
+L ++ PIE GII NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ +
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127
Query: 481 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 660
+ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + ++ G
Sbjct: 128 FYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGG 187
Query: 661 RDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEM 795
RD+TDYL L + G+ ++AE EIVR+IKEK CYVA DF E+
Sbjct: 188 RDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEI 232
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 250 bits (613), Expect = 2e-67
Identities = 127/270 (47%), Positives = 177/270 (65%), Gaps = 9/270 (3%)
Frame = +1
Query: 130 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSKRG 303
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++ R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 304 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 483
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM + M
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123
Query: 484 FETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGR 663
FE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + RLD+AGR
Sbjct: 124 FERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGR 183
Query: 664 DLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEMATXAASTSLEKSTNFL 843
D T YL+ +L +GY+F TA+ E VR++KEKLCYV+ D E + +T L ++
Sbjct: 184 DATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLMRNYTLP 243
Query: 844 TVR----*SHRYEKFRC---PRLXSAFLPG 912
R S RYE C P L + PG
Sbjct: 244 DGRVIKVGSERYECPECLFQPHLVGSEQPG 273
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 170 bits (414), Expect = 2e-43
Identities = 98/253 (38%), Positives = 139/253 (54%), Gaps = 32/253 (12%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 264
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 265 ------DSYVGDEAQSKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 423
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 424 LLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRT--------TGIVLDS 579
LLTE PLNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DS
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDS 186
Query: 580 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEK 759
GDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R + + IKE+
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRN---EPDSSLKTAERIKEE 243
Query: 760 LCYVALDFEXEMA 798
CYV D E +
Sbjct: 244 CCYVCPDIVKEFS 256
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 121 bits (291), Expect = 2e-28
Identities = 74/260 (28%), Positives = 135/260 (51%), Gaps = 3/260 (1%)
Frame = +1
Query: 58 AAVESIPASPTQTXTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 237
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R +
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60
Query: 238 GVMVGMGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEE 414
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + E
Sbjct: 61 K----LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRME 115
Query: 415 HPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDG 588
HP+ +TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+
Sbjct: 116 HPICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNA 175
Query: 589 VSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCY 768
SH +P+ G + R+ G + YL+K+ + SF + C+
Sbjct: 176 ASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSFPIKMLPSQAELLMHDHCH 235
Query: 769 VALDFEXEMATXAASTSLEK 828
V+ D+ ++A LE+
Sbjct: 236 VSSDYTHDIAHALDRDILER 255
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 113 bits (271), Expect = 4e-26
Identities = 63/196 (32%), Positives = 101/196 (51%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 300
D+V+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 301 GILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQI 480
G + +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 62 G-MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 481 MFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAG 660
MFE+ PA Y+A Q + +ASG+ T ++D G S IY+G+ L +G
Sbjct: 121 MFESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVLQKGYQVQHFSG 180
Query: 661 RDLTDYLMKILTERGY 708
+ D L + L ++ +
Sbjct: 181 NAINDILAQTLRDKNF 196
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 100 bits (239), Expect = 3e-22
Identities = 54/200 (27%), Positives = 103/200 (51%), Gaps = 2/200 (1%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 300
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 301 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 474
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 475 QIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDL 654
+ FE + PA Y+ QAV +A+ ++T +++D G + P+ +G + I + L
Sbjct: 122 EAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVDGLIIRKGIFKQSL 181
Query: 655 AGRDLTDYLMKILTERGYSF 714
AG L + ++ F
Sbjct: 182 AGDFLNANIEQLFNTMNIEF 201
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 74.5 bits (175), Expect = 2e-14
Identities = 62/238 (26%), Positives = 109/238 (45%), Gaps = 18/238 (7%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 315
+V+DNG+ KAGFAG P+ + R + G + ++G+E + TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 316 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTQIM 483
++ H G + +W +W N + P ++ +LLT+ + Q++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 484 FETFNSPAMYVAIQAVLSLYASGRTT--------------GIVLDSGDGVSHTVPIYEGY 621
FE F A A L + G T +V+DSG +H +P+ +
Sbjct: 118 FEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVIDSGYSFTHIIPVIDFS 177
Query: 622 ALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEXEIVRDIKEKLCYVALDFEXEM 795
A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE +CYV+ +F+ +M
Sbjct: 178 VQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKESVCYVSQNFKEDM 233
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 68.1 bits (159), Expect = 2e-12
Identities = 43/149 (28%), Positives = 70/149 (46%), Gaps = 4/149 (2%)
Frame = +1
Query: 322 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 489
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 490 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDL 669
P +A + ++ LYA G G+V+D G + PI +G + A +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231
Query: 670 TDYLMKILTERGYSFTTTAEXEIVRDIKE 756
T +L +L E + ++ + DI E
Sbjct: 232 TQHLQNLLRESLPTLKSSGQYVSKEDITE 260
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 37.9 bits (84), Expect = 0.002
Identities = 28/146 (19%), Positives = 67/146 (45%), Gaps = 12/146 (8%)
Frame = +1
Query: 313 LKYPIEHGIITNWD---------DMEKIWHHTFYNELRVAPEE---HPVLLTEAPLNPKA 456
L YPI+ G+ D D+ +I+ ++ + L++ + + V+ L +
Sbjct: 198 LFYPIKSGVFNESDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRV 257
Query: 457 NREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHA 636
EK+ I+F + + +++ + + +G + V+D G + + EG +P++
Sbjct: 258 YVEKILDILFFDLHFGKAAIVQESLCTSFGAGMSAACVVDMGAQKTSISCVEEGVVVPNS 317
Query: 637 ILRLDLAGRDLTDYLMKILTERGYSF 714
++++ G D+T MK+L + +
Sbjct: 318 RIKINYGGDDITLLFMKLLMRSHFPY 343
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.23
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +1
Query: 181 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 324
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.40
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 382 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 525
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 608 STKVTLCPTPSSVWTW 655
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 6.5
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 424 LLTEAPLNPKANREKMTQIMFETFN 498
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,270,092
Number of Sequences: 5004
Number of extensions: 64126
Number of successful extensions: 241
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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