Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_P09
(907 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 125 1e-29
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 45 1e-05
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 36 0.008
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.69
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 1.2
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 28 1.6
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 3.7
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 27 4.8
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 4.8
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 4.8
SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC su... 27 4.8
SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 6.4
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 6.4
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 6.4
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 8.5
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 8.5
SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase Ubp16|Schizo... 26 8.5
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 8.5
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 125 bits (301), Expect = 1e-29
Identities = 72/176 (40%), Positives = 98/176 (55%), Gaps = 10/176 (5%)
Frame = +1
Query: 280 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 456
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 457 MHGETPYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTL 615
M Y IMFGPD CG + VH IF +K + HL + T+LYTL
Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206
Query: 616 IVKPDNTYEVLIDNEKVESGDLXADW--DFLPPKKIKDPEAKKPEDWDDKPTIPDP 777
IVKPD T+EV I+ + V G L D+ LPP +I DPE KP DW D+P IPDP
Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADWVDEPEIPDP 262
Score = 47.6 bits (108), Expect = 2e-06
Identities = 28/65 (43%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = +1
Query: 688 DWDFLPPKKIKDPEAKKPEDW-DDKPT-IPDPENNKX*XLGQA*THSXSXCPQPXNWDDX 861
DWD P+ I DP+A KPEDW +D+P IPDPE K P +WDD
Sbjct: 269 DWDEDAPRMIPDPDAVKPEDWLEDEPLYIPDPEAQK-----------------PEDWDDE 311
Query: 862 MDGKW 876
DG W
Sbjct: 312 EDGDW 316
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 45.2 bits (102), Expect = 1e-05
Identities = 52/194 (26%), Positives = 82/194 (42%), Gaps = 8/194 (4%)
Frame = +1
Query: 160 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 327
F E+F + +W S W K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 328 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 501
P + + P+V+ F VK + CG Y+ + + K++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 502 CGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 675
CG I SY GK HL + T +YTL+++ + T + D V +G
Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTG 207
Query: 676 DLXADWDFLPPKKI 717
D+ ++ P K I
Sbjct: 208 DVGTNFFHSPTKWI 221
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 35.9 bits (79), Expect = 0.008
Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
Frame = -3
Query: 314 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQFIE 138
N SSE F S+S SL P VN + S + ++ L S + S+ NF+S ++S +
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531
Query: 137 DNASKLTTTSTT 102
+ S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 29.5 bits (63), Expect = 0.69
Identities = 22/68 (32%), Positives = 27/68 (39%), Gaps = 4/68 (5%)
Frame = -3
Query: 245 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT----TSTTAFIFDSQY 78
N N F + + S +SGNFS T Q DN S +T ST F S
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFSTQPGRLSTGLHHFPSDR 634
Query: 77 HLLQTKDR 54
LL + R
Sbjct: 635 DLLSNQTR 642
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1
Query: 700 LPPKKIKDPEAKKPEDWDDKPTIP 771
LP + K P AKKPED++ P P
Sbjct: 168 LPSPEYKAPPAKKPEDYEASPGEP 191
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/77 (24%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Frame = +1
Query: 538 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVES-GDLXADWDFLPPKK 714
S + K H KK +D Y+H+Y + P + + + ++ ES + D+LP
Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196
Query: 715 IKDPEAKKPEDWDDKPT 765
+K + P+D P+
Sbjct: 197 MKQKKG-GPKDSSKHPS 212
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 27.1 bits (57), Expect = 3.7
Identities = 34/140 (24%), Positives = 57/140 (40%)
Frame = -3
Query: 446 SSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSGS 267
+SL S P S S + T ++ +PS + + A ++SS PLSSS
Sbjct: 12 TSLSSSASSSIPASSSSAAASTSLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSF 71
Query: 266 LKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTTTSTTAFIFD 87
PA S T L+S SS SS +++ +++ LT++S+ +
Sbjct: 72 TS--PA----------SSSFITSLVS--SSSQQSSSSSASLTSSSSATLTSSSSASPTSS 117
Query: 86 SQYHLLQTKDRPRIINLKDS 27
S H L + + + S
Sbjct: 118 SSSHALSSSSSSLVASSSSS 137
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/45 (24%), Positives = 19/45 (42%)
Frame = +1
Query: 628 DNTYEVLIDNEKVESGDLXADWDFLPPKKIKDPEAKKPEDWDDKP 762
D E+ ++N ++ + WD PP + K+ WD P
Sbjct: 198 DKKRELELNNTEISQKPKRSRWDQAPPSVTQVSTTKRRSRWDKAP 242
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 4.8
Identities = 33/137 (24%), Positives = 59/137 (43%)
Frame = -3
Query: 506 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 327
P S I S SP S SS S + S S + + + T+ + + + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392
Query: 326 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQ 147
+ + SS + SSS S + V+ F ++ S ++ SH SS + SS ++
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHN----STSSRSSSHSSSHSLSSLSSKP 448
Query: 146 FIEDNASKLTTTSTTAF 96
+ ++S L T+S+ +
Sbjct: 449 ILTASSSSLLTSSSHTY 465
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 4.8
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 6/71 (8%)
Frame = +1
Query: 409 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 576
G +LKV + E M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 577 R--CKDDVYTH 603
CK+D + +
Sbjct: 674 LLFCKEDEFLY 684
>SPAC17H9.20 |psc3|SPAC607.01|mitotic cohesin complex, non-SMC
subunit Psc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 962
Score = 26.6 bits (56), Expect = 4.8
Identities = 14/54 (25%), Positives = 24/54 (44%)
Frame = +1
Query: 586 DDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLXADWDFLPPKKIKDPEAKKPED 747
D +Y L +L+ + N+YE + + + W FL D E KK ++
Sbjct: 753 DIIYERLVSLLSRVPNSYEDTLKFSAINTLQSLLFWFFLRKDNPADEEKKKDDE 806
>SPAC23G3.05c |||regulator of G-protein signaling
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 343
Score = 26.2 bits (55), Expect = 6.4
Identities = 17/69 (24%), Positives = 33/69 (47%)
Frame = +1
Query: 421 LKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 600
+K+F + +K + +P+E+M P + P K H + ++R +DV T
Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204
Query: 601 HLYTLIVKP 627
+L ++KP
Sbjct: 205 YLLNFLLKP 213
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.4
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -3
Query: 314 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 219
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 6.4
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 166 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 309
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 8.5
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = -3
Query: 338 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 177
++LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 8.5
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 163 EEKFPDDSWESNWVYS 210
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPCC1682.12c |ubp16||ubiquitin C-terminal hydrolase
Ubp16|Schizosaccharomyces pombe|chr 3|||Manual
Length = 457
Score = 25.8 bits (54), Expect = 8.5
Identities = 11/26 (42%), Positives = 15/26 (57%), Gaps = 1/26 (3%)
Frame = +1
Query: 697 FLPPKKIKDPEAKKPEDWDD-KPTIP 771
F+PP +I PE K +WD+ P P
Sbjct: 100 FIPPARILFPEEKLSMEWDNIMPNAP 125
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 8.5
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 688 DWDFLPPKKIKDPEAKKPEDW 750
DWD +P ++ K EA KP DW
Sbjct: 209 DWDDVPWRE-KPLEAMKPRDW 228
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,867,633
Number of Sequences: 5004
Number of extensions: 86805
Number of successful extensions: 302
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 297
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 458501510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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