Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_O19
(914 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 295 7e-81
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 164 2e-41
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 163 3e-41
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 139 6e-34
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 82 1e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 82 1e-16
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 77 3e-15
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 60 4e-10
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 40 4e-04
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 30 0.40
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.9
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.5
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 6.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 295 bits (723), Expect = 7e-81
Identities = 147/207 (71%), Positives = 159/207 (76%)
Frame = +2
Query: 251 VMVGMGQKDSYVGDEAQSXRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 430
+MVGMGQKDSYVGDEAQS RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 431 VLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHT 610
LLTEAPLNPK+NREKMTQI+FETFN+PA YVAIQAVLSLYASGRTTGIVLDSGDGV+HT
Sbjct: 103 CLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT 162
Query: 611 VPIYXGYALPHAILRLDLAGRXLTGLXHEESSPXGVTPFXTTAXKGNXSXHQGKXLLCPP 790
VPIY GYALPHAI+RLDLAGR LT + G T F TTA + K LC
Sbjct: 163 VPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYT-FSTTAER--EIVRDIKEKLCYV 219
Query: 791 SXSXXKMAPXXASTXPXXXSYXLPDRQ 871
+ + A + SY LPD Q
Sbjct: 220 ALDFEQELQTAAQSSSLEKSYELPDGQ 246
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +1
Query: 127 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 249
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 164 bits (398), Expect = 2e-41
Identities = 72/137 (52%), Positives = 102/137 (74%)
Frame = +2
Query: 272 KDSYVGDEAQSXRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 451
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 452 LNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGY 631
+NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+Y
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169
Query: 632 ALPHAILRLDLAGRXLT 682
L H + RLD+AGR T
Sbjct: 170 VLNHLVGRLDVAGRDAT 186
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +1
Query: 139 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 237
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 163 bits (396), Expect = 3e-41
Identities = 75/145 (51%), Positives = 105/145 (72%), Gaps = 1/145 (0%)
Frame = +2
Query: 251 VMVGMGQKDSYVGDEAQSXRGILTLKYPIEHGIITNWDDMEKIWHHTFYNE-LRVAPEEH 427
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + ++ L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 428 PVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSH 607
P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ GDG++H
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 608 TVPIYXGYALPHAILRLDLAGRXLT 682
+VPIY G+++P AI + ++ GR +T
Sbjct: 167 SVPIYDGFSIPSAIQQEEIGGRDVT 191
Score = 52.8 bits (121), Expect = 7e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +1
Query: 133 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 246
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 139 bits (336), Expect = 6e-34
Identities = 74/176 (42%), Positives = 104/176 (59%), Gaps = 12/176 (6%)
Frame = +2
Query: 191 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSXRGI-LTLKYPIEHGIITN 358
A++ PA SS+PS++A + D ++G++A +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 359 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQA 538
WD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE+FN +Y+A+QA
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQA 161
Query: 539 VLSLYASGRT--------TGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRXLT 682
VL+L AS + TG V+DSGDGV+H +P+ GY + +I + LAGR +T
Sbjct: 162 VLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGRDVT 217
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 228
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 81.8 bits (193), Expect = 1e-16
Identities = 42/139 (30%), Positives = 77/139 (55%), Gaps = 3/139 (2%)
Frame = +2
Query: 263 MGQKDSYVGDEAQSXRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 439
+ + + VG++ G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 440 TEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTV 613
TE NP R MT+++FE +N+P++ I + S Y + + ++GIVL+ G+ SH +
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVI 180
Query: 614 PIYXGYALPHAILRLDLAG 670
P+ G + R+ G
Sbjct: 181 PVLNGERILSEAKRISWGG 199
Score = 36.7 bits (81), Expect = 0.005
Identities = 17/42 (40%), Positives = 27/42 (64%)
Frame = +1
Query: 115 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 240
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 81.8 bits (193), Expect = 1e-16
Identities = 39/107 (36%), Positives = 58/107 (54%)
Frame = +2
Query: 317 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 496
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 497 ETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYAL 637
E+ PA Y+A Q + +ASG+ T ++D G S IY G+ L
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +1
Query: 130 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 303
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 77.4 bits (182), Expect = 3e-15
Identities = 40/138 (28%), Positives = 72/138 (52%), Gaps = 2/138 (1%)
Frame = +2
Query: 272 KDSYVGDEAQSXRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 445
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 446 APLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYX 625
NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D G + P+
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPVVD 168
Query: 626 GYALPHAILRLDLAGRXL 679
G + I + LAG L
Sbjct: 169 GLIIRKGIFKQSLAGDFL 186
Score = 34.3 bits (75), Expect = 0.024
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +1
Query: 130 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 231
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 60.1 bits (139), Expect = 4e-10
Identities = 37/121 (30%), Positives = 57/121 (47%), Gaps = 4/121 (3%)
Frame = +2
Query: 332 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 499
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 500 TFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYXGYALPHAILRLDLAGRXL 679
P +A + ++ LYA G G+V+D G + PI G + A +L L GR +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLGGRYM 231
Query: 680 T 682
T
Sbjct: 232 T 232
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 40.3 bits (90), Expect = 4e-04
Identities = 35/157 (22%), Positives = 62/157 (39%), Gaps = 18/157 (11%)
Frame = +2
Query: 281 YVGDEAQSXRGILTLKYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEA 448
++G+E + TL++ H G + +W +W N + P ++ +LLT+
Sbjct: 43 FLGNELANCNDFTTLQFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQP 102
Query: 449 PLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTG--------------IVLD 586
+ Q++FE F A A L + G T +V+D
Sbjct: 103 VFTMPSIEHNTIQLVFEEFQFDAYLPCTPAELIPWDHGSFTMNQEDAYTGQHGECVLVID 162
Query: 587 SGDGVSHTVPIYXGYALPHAILRLDLAGRXLTGLXHE 697
SG +H +P+ A+ R+D+ G+ LT E
Sbjct: 163 SGYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKE 199
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 30.3 bits (65), Expect = 0.40
Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +2
Query: 392 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNSPAM---YVAIQ 535
+Y L E+HP+LLT+ A L P+ + ++ +I ++ N+P + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 618 STKVTLCPTPSSVWTW 665
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.5
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -2
Query: 355 GDDTVLDGVLQSEDTSXALGLVTHVGVFLAHTDHHXPG 242
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 6.5
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 434 LLTEAPLNPKANREKMTQIMFETFN 508
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,011,081
Number of Sequences: 5004
Number of extensions: 55177
Number of successful extensions: 192
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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