Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_O13
(899 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC328.10c |rps502|rps5-2|40S ribosomal protein S5|Schizosaccha... 252 4e-68
SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces p... 250 2e-67
SPAC16E8.10c |||mitochondrial ribosomal protein subunit S7|Schiz... 38 0.003
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.90
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 27 2.7
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 27 3.6
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 27 3.6
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 27 4.8
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 26 8.4
>SPAC328.10c |rps502|rps5-2|40S ribosomal protein
S5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 203
Score = 252 bits (618), Expect = 4e-68
Identities = 129/196 (65%), Positives = 157/196 (80%)
Frame = +3
Query: 153 AGSVVVETMSLPQAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 332
A S++ + +SL + IKLF ++ V+V D+SL DYI++ + LPH+AGR+
Sbjct: 2 AASIIPKEVSLDETG---HIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQ 56
Query: 333 HKRFRKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAII 512
KRFRKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+
Sbjct: 57 TKRFRKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVA 116
Query: 513 NSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECVADEL 692
GPREDSTRIG AGTVRRQAVDVSPLRRVNQA+ L+ GAREAAFRN+K+I+EC+A+E+
Sbjct: 117 ACGPREDSTRIGSAGTVRRQAVDVSPLRRVNQALALITIGAREAAFRNVKSISECLAEEI 176
Query: 693 INAAKGSSNSYAHPKR 740
INAAKGSSNSYA K+
Sbjct: 177 INAAKGSSNSYAIKKK 192
Score = 31.5 bits (68), Expect = 0.17
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = +2
Query: 734 KKKDELERVAKSNR 775
KKKDELERVAKSNR
Sbjct: 190 KKKDELERVAKSNR 203
>SPAC8C9.08 |rps5||40S ribosomal protein S5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 203
Score = 250 bits (612), Expect = 2e-67
Identities = 129/192 (67%), Positives = 153/192 (79%), Gaps = 3/192 (1%)
Frame = +3
Query: 174 TMSLPQAADIPE---IKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYAHKRF 344
T SL + E IKLF ++ V+V D+SL DYI++ + LPH+AGR+ KRF
Sbjct: 3 TSSLTPGVSLDENGSIKLFNKFPFEGVEVKDISLVDYITIGN--GQPLPHTAGRFQTKRF 60
Query: 345 RKAQCPIVERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 524
RKA+C IVERLTNSLMM+GRNNGKKL+A RIVKHAFEII LLT +NPLQVLV A+ GP
Sbjct: 61 RKARCFIVERLTNSLMMNGRNNGKKLLATRIVKHAFEIIALLTDQNPLQVLVDAVAACGP 120
Query: 525 REDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECVADELINAA 704
REDSTRIG AGTVRRQAVDVSPLRRVNQA+ L+ GAREAAFRN+K+I+EC+A+E+INAA
Sbjct: 121 REDSTRIGSAGTVRRQAVDVSPLRRVNQALALITIGAREAAFRNVKSISECLAEEIINAA 180
Query: 705 KGSSNSYAHPKR 740
KGSSNSYA K+
Sbjct: 181 KGSSNSYAIKKK 192
Score = 31.5 bits (68), Expect = 0.17
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = +2
Query: 734 KKKDELERVAKSNR 775
KKKDELERVAKSNR
Sbjct: 190 KKKDELERVAKSNR 203
>SPAC16E8.10c |||mitochondrial ribosomal protein subunit
S7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 259
Score = 37.5 bits (83), Expect = 0.003
Identities = 23/53 (43%), Positives = 28/53 (52%)
Frame = +3
Query: 366 VERLTNSLMMHGRNNGKKLMAVRIVKHAFEIIHLLTGENPLQVLVTAIINSGP 524
V+ L N +M +GKK A +IV A II TGENP+ VL AI P
Sbjct: 126 VQHLVNLIM----RDGKKAKAEKIVATALSIIQKETGENPIDVLKQAIAEISP 174
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 29.1 bits (62), Expect = 0.90
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2
Query: 425 GRTYCQTCV*NYSLVNWRKPSASTRDCHYQLWT 523
G TYC C L+NW K S S C +L+T
Sbjct: 101 GHTYCYEC-----LLNWLKESKSCPTCRQKLYT 128
>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 335
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +3
Query: 447 AFEIIHLLTGENPLQVLVTAIINSGPREDSTRIGRAGTVRRQAVDVSPLRR 599
+ +I HL+TG N + T ++N R D R+ R+ + Q+ D SP +R
Sbjct: 159 SLDIHHLVTGHNADDIAETILMNL-LRGDVARLPRSTEITTQS-DSSPTKR 207
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +3
Query: 189 QAADIPEIKLFGRWSCYDVQVSDMSLQDYISVKEKYAKYLPHSAGRYA 332
+ ADI + FGR ++++++ + I+V+EKYAK P R A
Sbjct: 7 KVADI-SLAAFGR---KELEIAENEMPGLIAVREKYAKSQPLKGARIA 50
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 27.1 bits (57), Expect = 3.6
Identities = 9/30 (30%), Positives = 17/30 (56%)
Frame = +3
Query: 663 TIAECVADELINAAKGSSNSYAHPKRRTSW 752
++ C +L++ +G+ NS+ HP R W
Sbjct: 458 SLLPCTEMKLVDCEEGNYNSHGHPPRGEIW 487
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +1
Query: 517 LDPVKIRLGSVVRVQFVVKPLMFHPCAESTKQSGFCAQV 633
++PV R SVV V +P+ +H +S ++G Q+
Sbjct: 273 IEPVSSRQSSVVNNNSVQQPVAYHAFVQSPTENGTLPQL 311
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = +2
Query: 374 PYKLSNDARSEQWQKTDGRTYCQTCV*NYSLVNWRKPSAST 496
PYK+ + + W + G+TY + + + L R+ +AST
Sbjct: 138 PYKIVEHSNGDAWLEARGKTYSPSQIGGFILSKMRE-TAST 177
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,444,371
Number of Sequences: 5004
Number of extensions: 69637
Number of successful extensions: 168
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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