Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_O05
(897 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 182 5e-47
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 93 5e-20
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 30 0.51
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 2.1
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 27 2.7
SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe... 27 2.7
SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr 2|... 27 2.7
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.7
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 27 3.6
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 4.8
SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces pom... 26 8.4
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 26 8.4
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 182 bits (444), Expect = 5e-47
Identities = 90/159 (56%), Positives = 110/159 (69%), Gaps = 7/159 (4%)
Frame = +1
Query: 100 DRALFNGAQKLFGMVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDE 279
DR ++ QK ++I GQA YV+TGMYG P ++GAG+CLL+I+QL A LIVLLLDE
Sbjct: 110 DREMYQLVQKFLAIIIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDE 169
Query: 280 LLQKGYGLGSGISLFIATNICETIVWKAFSPATVNTGRGTEFEGAVIALFHLLATRPDKV 459
LLQKGYGLGSGISLFIAT CE I WKAFSP T + G +FEGAVI +++ T +K
Sbjct: 170 LLQKGYGLGSGISLFIATINCENIFWKAFSPTTYHIANGVQFEGAVINFVYVMFTWDNKA 229
Query: 460 RALREAFYRQ-------NLPNLMNLLATVLVFAIVIYFQ 555
AL +AF+R LPNL N AT+LVF +VIY Q
Sbjct: 230 AALYQAFFRSGLTSSQIQLPNLWNFFATLLVFGVVIYLQ 268
Score = 83.8 bits (198), Expect = 3e-17
Identities = 42/86 (48%), Positives = 55/86 (63%)
Frame = +2
Query: 560 FRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLAVKFXGNFLXNV 739
FRV++PI+S ++RG S++P+KL YTSN PI+LQSAL SNL+ S++L +F NFL
Sbjct: 270 FRVEIPIRSQKFRGYRSTFPVKLLYTSNTPIMLQSALTSNLFFASRLLFNRFSSNFLVRF 329
Query: 740 LGVWADXGGGXPAXXAPVGGLCYYLS 817
LGVW GL YYLS
Sbjct: 330 LGVWEQ---------TATSGLSYYLS 346
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 93.1 bits (221), Expect = 5e-20
Identities = 47/152 (30%), Positives = 86/152 (56%), Gaps = 2/152 (1%)
Frame = +1
Query: 100 DRALFNGAQKLFGMVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDE 279
DR LF AQK+ ++ + A+ YV +G YG S++G +++I+Q+F+ G++ + L E
Sbjct: 110 DRVLFQNAQKVVSALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPGIVCIYLCE 169
Query: 280 LLQKGYGLGSGISLFIATNICETIVWKAFS--PATVNTGRGTEFEGAVIALFHLLATRPD 453
+++KG+GLGSG L + ++I I+W S VN ++++GA++ L + +
Sbjct: 170 IIEKGHGLGSGPVLLLGSHILGNIMWDVLSLHRYPVNESGDSQYQGALVGFAFNLFSFKN 229
Query: 454 KVRALREAFYRQNLPNLMNLLATVLVFAIVIY 549
K +LR +R + + L + VFA +Y
Sbjct: 230 KFSSLRSILFRSEGLSFVQFLVCIAVFATFMY 261
Score = 52.4 bits (120), Expect = 8e-08
Identities = 28/84 (33%), Positives = 47/84 (55%)
Frame = +2
Query: 563 RVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLAVKFXGNFLXNVL 742
R+D+PI+S+R RG ++P+KL YTS IP+I +++S+L V + L + + +L
Sbjct: 266 RIDVPIRSSRVRGVRQNFPLKLLYTSVIPLIYFYSILSHLLVFAYALYSLCPNSLITRLL 325
Query: 743 GVWADXGGGXPAXXAPVGGLCYYL 814
++ VGGL Y+L
Sbjct: 326 VQYSPIDTFAEHKLQLVGGLVYFL 349
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 29.9 bits (64), Expect = 0.51
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -2
Query: 335 FVAINREIPEPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAPIS 201
+ +I+RE+ N PF SNS S ++ T++ +R + +PI+
Sbjct: 29 YTSISRELDYANQSPFSSNSFSPTELKARTSATFDVRSASTSPIN 73
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -2
Query: 323 NREIPEPNPYPFWSNSSSNNT 261
N +P P P P +NSS+NNT
Sbjct: 395 NSNMPPPAPRPVTNNSSNNNT 415
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -1
Query: 321 QRDTRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHYINNSLTN 151
Q+ +S S ++ F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 150 SNHHTK 133
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 365
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 2/53 (3%)
Frame = +1
Query: 343 ETIVWKAFSPATVNTGRGTEFEGAVIALFHLLATRPD--KVRALREAFYRQNL 495
E +VW+ F TV TG G I ++L R + K RA + Y + L
Sbjct: 273 EDVVWETFDSQTVETGNGELCAANFIPAVYVLNQRKEEKKKRAKDDEQYAKRL 325
>SPBC2A9.08c |sec22||SNARE Sec22|Schizosaccharomyces pombe|chr
2|||Manual
Length = 209
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/69 (24%), Positives = 26/69 (37%)
Frame = -1
Query: 351 NGFTDVCSNKQRDTRTQSISFLEQFIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHY 172
NG +C +Q R + S+LE+ + N G + L ++ F F S
Sbjct: 59 NGICYLCICEQSYPRKLAFSYLEELAGEFWNSFGEEALQPGLRPYAFVQFDTFMQKSKRV 118
Query: 171 INNSLTNSN 145
N N N
Sbjct: 119 YNTPRANDN 127
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -1
Query: 258 KTGHKQLYDNEEAHSGTNFTGFAIHSSHYINN-SLTNSNHHT 136
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 27.1 bits (57), Expect = 3.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -2
Query: 479 KASRRARTLSGRVANRWNNAITAPS 405
K RR+R LS ++ N W+ + +PS
Sbjct: 542 KLFRRSRELSEKLCNNWSERVKSPS 566
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 26.6 bits (56), Expect = 4.8
Identities = 20/76 (26%), Positives = 33/76 (43%), Gaps = 11/76 (14%)
Frame = -2
Query: 428 NNAITAPSNSVPRPVLTVAGENAFH-----------TMVSQMFVAINREIPEPNPYPFWS 282
N +++P NS PRP+ V+ A + + VS +A N++ P P P P
Sbjct: 261 NGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRR 320
Query: 281 NSSSNNTIRPATNSCM 234
N ++NS +
Sbjct: 321 NRGKPPIGNGSSNSSL 336
>SPAC4G8.04 |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 772
Score = 25.8 bits (54), Expect = 8.4
Identities = 18/53 (33%), Positives = 25/53 (47%), Gaps = 1/53 (1%)
Frame = -2
Query: 347 VSQMFVAINREIPEPNPYPFWS-NSSSNNTIRPATNSCMIMRRHTPAPISLGS 192
VS FV+ E PE Y + NSSSN +R + + + P+SL S
Sbjct: 288 VSSTFVSATSE-PEVETYSVSTKNSSSNKNLRSSLSKLLSTSNLNNKPLSLSS 339
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 25.8 bits (54), Expect = 8.4
Identities = 18/72 (25%), Positives = 31/72 (43%), Gaps = 5/72 (6%)
Frame = -1
Query: 441 CQQMEQCYHSTLEFCAA-----TSVNSGW*KCLPYNGFTDVCSNKQRDTRTQSISFLEQF 277
C M C EFC T + W C N + + S RD++++S + LE++
Sbjct: 304 CNHMT-CKKCKYEFCWVCLGPWTEHGNNWYTC---NRYEEKSSTSARDSQSKSRASLERY 359
Query: 276 IEQQHNKTGHKQ 241
+ + H+Q
Sbjct: 360 LHYYNRFANHEQ 371
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,490,925
Number of Sequences: 5004
Number of extensions: 76952
Number of successful extensions: 220
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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