Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_N19
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 370 e-103
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 211 9e-56
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 210 3e-55
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 196 3e-51
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.6
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 4.7
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 6.3
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 6.3
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 370 bits (911), Expect = e-103
Identities = 168/244 (68%), Positives = 194/244 (79%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 316
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 317 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 496
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 497 RKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 676
R+EAE+CD LQGFQ KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180
Query: 677 EPYNATLSVHQLVENTDETYCIDNXALYDICFRTLKLSTPTYGXLNHLVSLTMSGVTTCL 856
EPYNATLS+HQLVEN+DET+CIDN AL I TLK+ +P+Y LNHLVS M+GVTT
Sbjct: 181 EPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSF 240
Query: 857 RFPG 868
RFPG
Sbjct: 241 RFPG 244
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 211 bits (516), Expect = 9e-56
Identities = 100/251 (39%), Positives = 147/251 (58%), Gaps = 6/251 (2%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 298
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 299 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 478
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 479 SVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 658
SVL+ +R+ A++C LQGF ++ EY + +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Query: 659 VSDTVVEPYNATLSVHQLVENTDETYCIDNXALYDICFRTLKLSTPTYGXLNHLVSLTMS 838
VS +VVEPYN+ L+ H ++N+D T+ +DN A YDIC R L + PTY LN L++ +S
Sbjct: 181 VSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVS 240
Query: 839 GVTTCLRFPGT 871
+T LRF G+
Sbjct: 241 SITASLRFAGS 251
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 210 bits (512), Expect = 3e-55
Identities = 100/247 (40%), Positives = 144/247 (58%), Gaps = 2/247 (0%)
Frame = +2
Query: 137 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 310
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 311 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 490
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 491 VVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 670
+R+ A++C LQGF ++ EY + +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 671 VVEPYNATLSVHQLVENTDETYCIDNXALYDICFRTLKLSTPTYGXLNHLVSLTMSGVTT 850
VVEPYN+ L+ H ++ D T+ +DN + YDIC R L + P+Y LN L++ +S +T
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 851 CLRFPGT 871
LRF G+
Sbjct: 241 SLRFEGS 247
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 196 bits (478), Expect = 3e-51
Identities = 94/246 (38%), Positives = 145/246 (58%), Gaps = 3/246 (1%)
Frame = +2
Query: 140 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 319
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 320 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 493
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 494 VRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 670
+ +EA+ D L+GF ++ + YP +I+ TYSV P S VSD
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 671 VVEPYNATLSVHQLVENTDETYCIDNXALYDICFRTLKLSTPTYGXLNHLVSLTMSGVTT 850
VV+PYN+ L++ +L N D +DN AL I L PT+ N LVS MS TT
Sbjct: 182 VVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTT 241
Query: 851 CLRFPG 868
LR+PG
Sbjct: 242 TLRYPG 247
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 3.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 176 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 277
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 371 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 472
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 6.3
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +2
Query: 440 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 529
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 6.3
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -2
Query: 513 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 376
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +2
Query: 392 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 523
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,096,179
Number of Sequences: 5004
Number of extensions: 57369
Number of successful extensions: 181
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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