Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_N17
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 44 4e-05
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 40 5e-04
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 39 8e-04
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 31 0.29
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 30 0.51
SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyce... 30 0.51
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo... 30 0.51
SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein Mud1|Schizosacch... 29 0.67
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 29 0.89
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 27 2.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.6
SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.6
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 27 4.7
SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 compon... 27 4.7
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.3
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 6.3
SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizo... 26 6.3
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 26 8.3
SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr 3... 26 8.3
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 43.6 bits (98), Expect = 4e-05
Identities = 38/135 (28%), Positives = 49/135 (36%), Gaps = 4/135 (2%)
Frame = -3
Query: 677 APPMAVPIPP--NEVAMP-SRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPN 507
APP P P N ++ P S P S P S + S P +P + S P
Sbjct: 965 APPSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSKAPPV 1024
Query: 506 PESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNPES-CPKLPNPESRPK 330
P P P+ P P S P P+S P+ P P S P +P P P
Sbjct: 1025 PLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPP 1084
Query: 329 SPNPESRPKLSNPGL 285
P P P + P +
Sbjct: 1085 VPAPSGIPPVPKPSV 1099
Score = 30.3 bits (65), Expect = 0.38
Identities = 22/79 (27%), Positives = 33/79 (41%), Gaps = 3/79 (3%)
Frame = -3
Query: 683 VRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLS--SAESPKLP 510
V P A P+P V +P P + +P + P P + S+E+P +
Sbjct: 1191 VPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVS 1250
Query: 509 NPESWCPNPES-WCPNPES 456
P S P+P S P+P S
Sbjct: 1251 TPRSSVPSPHSNASPSPTS 1269
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 39.9 bits (89), Expect = 5e-04
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 3/65 (4%)
Frame = -3
Query: 467 NPESWCP-NPESWCPNPESCPKLPNPESWCPNPESCPKLP-NPESRPKSPNP-ESRPKLS 297
+P S P P+S P S P P P P P S P +P P S P P P S P +
Sbjct: 1685 HPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Query: 296 NPGLR 282
NPG R
Sbjct: 1745 NPGDR 1749
Score = 28.7 bits (61), Expect = 1.2
Identities = 21/58 (36%), Positives = 23/58 (39%), Gaps = 1/58 (1%)
Frame = -3
Query: 506 PESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNP-ESCPKLPNPESR 336
P+ P P P P P P S P P P P P P P S P +PNP R
Sbjct: 1700 PQMSAPTP----PPPPMSVPPPPSAPPMPAGPPSAPPP----PLPASSAPSVPNPGDR 1749
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -3
Query: 674 PPMAVPIPPNEVAMPSRPFSILSSSIPWSA 585
PPM+VP PP+ MP+ P S +P S+
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 39.1 bits (87), Expect = 8e-04
Identities = 27/82 (32%), Positives = 35/82 (42%), Gaps = 2/82 (2%)
Frame = -3
Query: 524 SPKLPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNPESCPKLPNP 345
+P P P+S P S P P P+P S P P P P P S P + + +P
Sbjct: 127 APAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAP--PIPSSLPPPAQPAAPVKSP 184
Query: 344 ESRPKSPN--PESRPKLSNPGL 285
S P P+ P PK+ P L
Sbjct: 185 PSAPSLPSAVPPMPPKVPPPPL 206
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 30.7 bits (66), Expect = 0.29
Identities = 25/81 (30%), Positives = 36/81 (44%), Gaps = 1/81 (1%)
Frame = -3
Query: 635 MPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWCPNPESWCPNPES 456
+P R FS+ S+S P + +S P P S + N +S+ +P+S NP S
Sbjct: 250 VPRRQFSVSSASDPPQTPISMSPPIP----PTPSQFSAFMYQNQQSYL-SPQSHYENPLS 304
Query: 455 WCPNPESWCPN-PESCPKLPN 396
P CP+ P S L N
Sbjct: 305 ISSRPSPCCPSTPSSAVTLSN 325
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 29.9 bits (64), Expect = 0.51
Identities = 23/79 (29%), Positives = 29/79 (36%), Gaps = 10/79 (12%)
Frame = -3
Query: 521 PKLPNPESWCPNPESWCPNPESWCP------NPESWCP----NPESCPKLPNPESWCPNP 372
P PE W + + P+PE+ P W P NP+ C + W P
Sbjct: 281 PDAVKPEDWLEDEPLYIPDPEAQKPEDWDDEEDGDWIPSEIINPK-CIEGAGCGEWKPPM 339
Query: 371 ESCPKLPNPESRPKSPNPE 315
P P S P PNPE
Sbjct: 340 IRNPNYRGPWSPPMIPNPE 358
>SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 533
Score = 29.9 bits (64), Expect = 0.51
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = -1
Query: 364 ARSFRIRSHVQSHRTQSHDRNYRIQGCDFQSRQIRNHDQNCRSRYDQN 221
AR + + SH+Q+H + DR YR C ++ +R+HD R +N
Sbjct: 455 ARKYNVESHIQTHLS---DRPYRCDLC--KAGFVRHHDLKRHLRIHEN 497
>SPBC19G7.10c |||topoisomerase associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 744
Score = 29.9 bits (64), Expect = 0.51
Identities = 27/106 (25%), Positives = 44/106 (41%), Gaps = 3/106 (2%)
Frame = -3
Query: 668 MAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWCP 489
M P PP++ A+P P S +++ + + IS PA+S + + + P +
Sbjct: 179 MTAPKPPSQPAIPIVP-SEMAAQV---TRENISSLDPAISAASIGNVTFGQPNIPSTTTD 234
Query: 488 NPESWCPN---PESWCPNPESWCPNPESCPKLPNPESWCPNPESCP 360
PN P PNP P P++P P++ NP P
Sbjct: 235 FAGLAAPNMVHPSQAIPNPVM---QPSLVPQMPYPQNGMYNPSVAP 277
>SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein
Mud1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 29.5 bits (63), Expect = 0.67
Identities = 23/80 (28%), Positives = 31/80 (38%)
Frame = -3
Query: 548 LPKLSSAESPKLPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNPE 369
+P L +E PKL N P S + PNP+S +L ES PN E
Sbjct: 223 IPFLGESEIPKLLANVEPSANAHGLGIEPAS---KASASSPNPQSGTRLGTKESVAPNNE 279
Query: 368 SCPKLPNPESRPKSPNPESR 309
P+ + P P S+
Sbjct: 280 GSSNPPSLVNPPTDPGLNSK 299
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 29.1 bits (62), Expect = 0.89
Identities = 33/111 (29%), Positives = 48/111 (43%), Gaps = 4/111 (3%)
Frame = -3
Query: 698 FAIFEVRAPPMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPK-LSSAES 522
F F R P P N ++PS P SSS P + + + + + +P L S++
Sbjct: 98 FKNFPFRPPTRLTSTPSNSSSLPSIP---SSSSTPSISSIPHTTSSVSNDIPSVLGSSDH 154
Query: 521 P-KLPNPESWCPNPESWCPNPE-SWCPNPESWCPNPESCPK-LPNPESWCP 378
P L NPE P P S+ + S P P P+ S P + S+CP
Sbjct: 155 PIDLDNPEHLTP-PSSFITAKQLSRLPTPLP-PPSSSSLPTGTISTNSFCP 203
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/63 (26%), Positives = 31/63 (49%)
Frame = -3
Query: 671 PMAVPIPPNEVAMPSRPFSILSSSIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWC 492
P + P PP + + S+P S SS++P K F+ + S ++++ P+ + ES
Sbjct: 193 PYSSPFPPPRIVLGSKPVS--SSTLPSKPKSFQVFSDASSSRDSQNASDLPQAKSLESEA 250
Query: 491 PNP 483
P
Sbjct: 251 NTP 253
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/51 (31%), Positives = 19/51 (37%)
Frame = -3
Query: 449 PNPESWCPNPESCPKLPNPESWCPNPESCPKLPNPESRPKSPNPESRPKLS 297
P PE P P P PE P P P PE+ +P S +S
Sbjct: 113 PVPEEPLPGEPPLPDEPVPEEPLPGEPPLPNEPVPETNCHKESPLSDETVS 163
>SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 224
Score = 27.1 bits (57), Expect = 3.6
Identities = 22/73 (30%), Positives = 27/73 (36%), Gaps = 1/73 (1%)
Frame = -3
Query: 533 SAESPKLPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCP-NPESCPK 357
S+ S LPN + N P S N S P P + P P SW P N +
Sbjct: 39 SSSSSTLPNYAASSLNSR---PVSSSGSGNAYSQAPYPPARPTSQRPNSWQPGNASTMYA 95
Query: 356 LPNPESRPKSPNP 318
P P S + P
Sbjct: 96 SPPPSSNYNTAKP 108
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 26.6 bits (56), Expect = 4.7
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 4/50 (8%)
Frame = -3
Query: 569 FTCP--ALSLPKLSSA--ESPKLPNPESWCPNPESWCPNPESWCPNPESW 432
+T P A+ +P L A +S P PE++ NP+ W PN + +P++W
Sbjct: 403 YTVPKDAMVIPTLYGALHDSKVYPEPETF--NPDRWAPNGLAE-QSPKNW 449
>SPBC776.15c |||dihydrolipoamide S-succinyltransferase, e2 component
of oxoglutarate dehydrogenase complex
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 26.6 bits (56), Expect = 4.7
Identities = 19/73 (26%), Positives = 30/73 (41%), Gaps = 1/73 (1%)
Frame = -3
Query: 542 KLSSAESPK-LPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNPES 366
K + A++ K L P+ P P P+ ES + + P PE P P+
Sbjct: 136 KTADADAAKDLSTPQD-SSKPIEEKPMPDLGAEQKESAPSSTKPAPDAKEPEFSSPKPKP 194
Query: 365 CPKLPNPESRPKS 327
P +S+PK+
Sbjct: 195 AKSEPVKQSKPKA 207
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.3
Identities = 19/69 (27%), Positives = 28/69 (40%)
Frame = -3
Query: 530 AESPKLPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPKLPNPESWCPNPESCPKLP 351
A SP++P ++ P P P P P + P + +LP P N S +P
Sbjct: 218 AVSPEIP--PTYTPKQADPLPAPPP--PPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIP 273
Query: 350 NPESRPKSP 324
P + P P
Sbjct: 274 PPPATPSQP 282
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/42 (35%), Positives = 18/42 (42%), Gaps = 1/42 (2%)
Frame = -3
Query: 422 PESCPKLPNPESWCPNPESCP-KLPNPESRPKSPNPESRPKL 300
PE+ P P P S P P S P L E+ P P+L
Sbjct: 196 PETVPSTPQPASSLPIPSSPPAALETEENVDSQPMELDEPEL 237
>SPBC32C12.02 |ste11|aff1, stex|transcription factor
Ste11|Schizosaccharomyces pombe|chr 2|||Manual
Length = 468
Score = 26.2 bits (55), Expect = 6.3
Identities = 21/63 (33%), Positives = 26/63 (41%), Gaps = 4/63 (6%)
Frame = -3
Query: 572 SFTCPALSLPKLSS----AESPKLPNPESWCPNPESWCPNPESWCPNPESWCPNPESCPK 405
S C A +PK S +E +PE S C +P P+ S CPN PK
Sbjct: 259 SSNCSARRVPKFDSKGTVSEQSNSDSPELSADKVLSHC-SPIDARPSTPS-CPNASISPK 316
Query: 404 LPN 396
PN
Sbjct: 317 TPN 319
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 25.8 bits (54), Expect = 8.3
Identities = 37/145 (25%), Positives = 56/145 (38%), Gaps = 7/145 (4%)
Frame = -3
Query: 776 NIVYTTREL*AVKQ--IVLTCXDQCLRPFAIFEVRAPPMAVPIPPNEVAMPSRPFSILSS 603
N+V T + V + IVL LR F FE P M +P P + F L S
Sbjct: 185 NLVLTFLDTTTVSKVNIVLEIHIPVLRDFVTFEQYIPLMFLPSPFETIL----SFDNLKS 240
Query: 602 SIPWSAKLEISFTCPALSLPKLSSAESPKLPNPESWCPNPESWCPNPES--WCPNPESWC 429
P S+ L + + +S +S + + L + N E+ W P P +
Sbjct: 241 DSP-SSDLHLGWD-HTVSKESMSQSIAEVLNPYNKGVSRSLNDATNMETYHWSPFPPQFS 298
Query: 428 PNPESCPKLP---NPESWCPNPESC 363
++ +L P+SW P SC
Sbjct: 299 TFNDNALRLRIYYKPKSWLPKNSSC 323
>SPCC5E4.05c |||serine hydrolase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 8.3
Identities = 14/37 (37%), Positives = 19/37 (51%), Gaps = 2/37 (5%)
Frame = -2
Query: 411 PEATESRVVVSKSRIVPEASESGVTSKVTE--PRVTT 307
P + R S+S VPE + T ++E PRVTT
Sbjct: 332 PASAAERAPTSESTTVPETIVASTTKVISEPAPRVTT 368
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,759,915
Number of Sequences: 5004
Number of extensions: 57377
Number of successful extensions: 231
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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