Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_M13
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 38 0.001
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 36 0.010
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.055
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.22
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 31 0.29
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 8.3
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 8.3
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 8.3
SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces pom... 23 8.8
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 38.3 bits (85), Expect = 0.001
Identities = 31/85 (36%), Positives = 32/85 (37%)
Frame = -3
Query: 656 GGGGPPXPXXGGXFPXGGGXKKKXSXXGGXFXXGGGKXGXFXXXPPXXPPXFXGGGGGXX 477
GG G P P GG F GG + GG GGG G P F GG GG
Sbjct: 195 GGSGGPPPGPGG-FGGFGGFGGEGHHHGGHGGFGGGPGGF-----EGGPGGFGGGPGGFG 248
Query: 476 FXXLGPFFXGGXRGXXXGGGGFFXP 402
G F GG G G GG P
Sbjct: 249 GGLGG--FGGGPGGFGGGPGGHGGP 271
Score = 31.9 bits (69), Expect = 0.13
Identities = 34/101 (33%), Positives = 34/101 (33%)
Frame = -3
Query: 575 GGXFXXGGGKXGXFXXXPPXXPPXFXGGGGGXXFXXLGPFFXGGXRGXXXGGGGFFXPPX 396
GG GGG G PP P F G GG F G GG G G GGF P
Sbjct: 188 GGFGGFGGGSGG-----PPPGPGGFGGFGG---FGGEG-HHHGGHGGFGGGPGGFEGGPG 238
Query: 395 XXKXAXXFFXXPPXXXPXGGGGXXXPRGGPXXXGXGGGXXG 273
F GGG GGP G G G G
Sbjct: 239 GFGGGPGGF----------GGGLGGFGGGPGGFGGGPGGHG 269
Score = 29.5 bits (63), Expect = 0.67
Identities = 32/98 (32%), Positives = 32/98 (32%)
Frame = -1
Query: 574 GGXXPXGGXKXGXFXXPPPXXPPXFXGGGGXXXSXXWXPFXWGGXGGXXXGGGXFXPPPX 395
GG GG G PP P F G GG GG GG G G F P
Sbjct: 188 GGFGGFGGGSGG-----PPPGPGGFGGFGGFGGEGHHH----GGHGGFGGGPGGFEGGPG 238
Query: 394 XXXXXXFFFXXPXXXXPXGGGGXXXPGGXPGXGGXGGG 281
F P GGG GG PG G G G
Sbjct: 239 G------FGGGPGGF----GGGLGGFGGGPGGFGGGPG 266
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 35.5 bits (78), Expect = 0.010
Identities = 14/23 (60%), Positives = 14/23 (60%), Gaps = 1/23 (4%)
Frame = +3
Query: 282 PPPXPPXPG-XPPGXXXPPPPXG 347
PPP PP PG PP PPPP G
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPG 31
Score = 29.9 bits (64), Expect = 0.51
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPPXGXXXXGXXKK 374
PP PP P PPG P P G KK
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGYVKK 35
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 1/27 (3%)
Frame = +1
Query: 274 PXXPPPXPXXXG-PPRGXXXPPPPXGF 351
P PPP P G P PPPP G+
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPPGY 32
Score = 27.9 bits (59), Expect = 2.1
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +2
Query: 419 PPXXXPPXXPPXKRXPXXXXXXPPPP 496
PP PP PP P PPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/36 (44%), Positives = 16/36 (44%)
Frame = +1
Query: 601 PPPXGXXPPXXGXGGPPPPXRGXAXPKKIKKXXPPQ 708
PPP G PP PPPP G KK K P Q
Sbjct: 13 PPPPGFEPPSQ---PPPPPPPGYV--KKRKNKTPAQ 43
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.1 bits (72), Expect = 0.055
Identities = 24/90 (26%), Positives = 27/90 (30%), Gaps = 2/90 (2%)
Frame = +1
Query: 415 PPPPXXXPLXPPXKKGPXXXXXXPPPPP--XXXGGXXGGXXKKXPXXPPPXXKXPPXXLX 588
PPP PP G PPPPP G + PPP + P
Sbjct: 315 PPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGR 374
Query: 589 FFFXPPPXGXXPPXXGXGGPPPPXRGXAXP 678
PPP PPP G + P
Sbjct: 375 ---QPPPLSSSRAVSNPPAPPPAIPGRSAP 401
Score = 32.7 bits (71), Expect = 0.072
Identities = 34/141 (24%), Positives = 37/141 (26%), Gaps = 13/141 (9%)
Frame = +1
Query: 274 PXXPPPXPXXXG----PPRGXXXPPPPXGFXXGGXXKKXXAXFXXXGGXKXPPPPXXXP- 438
P PPP G PP+G PPPP ++ P PP P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 439 -----LXPPXKKGPXXXXXXPPPPPXXXGGXXGGXXKKXPXXPPPXXKXPPXXLXFFFXP 603
L P P PP P P PP P
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAP 457
Query: 604 P-PXG--XXPPXXGXGGPPPP 657
P P G PP PPP
Sbjct: 458 PLPAGMPAAPPLPPAAPAPPP 478
Score = 28.7 bits (61), Expect = 1.2
Identities = 24/94 (25%), Positives = 27/94 (28%), Gaps = 3/94 (3%)
Frame = +1
Query: 400 GGXKXPPPPXXXPLXPPXKKGPXXXXXXPP-PPPXXX--GGXXGGXXKKXPXXPPPXXKX 570
G PP P+ P PP PPP K+ P PPP +
Sbjct: 262 GTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRN 321
Query: 571 PPXXLXFFFXPPPXGXXPPXXGXGGPPPPXRGXA 672
PP G PPPP R A
Sbjct: 322 R--------GKPPIGNGSSNSSLPPPPPPPRSNA 347
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/53 (26%), Positives = 16/53 (30%)
Frame = +1
Query: 415 PPPPXXXPLXPPXKKGPXXXXXXPPPPPXXXGGXXGGXXKKXPXXPPPXXKXP 573
PP P P P ++ P PPP P G P P P
Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNP 282
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.1 bits (67), Expect = 0.22
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPP 341
PPP PP PG PPPP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPP 780
Score = 30.3 bits (65), Expect = 0.38
Identities = 20/68 (29%), Positives = 22/68 (32%)
Frame = +1
Query: 535 KXPXXPPPXXKXPPXXLXFFFXPPPXGXXPPXXGXGGPPPPXRGXAXPKKIKKXXPPQXX 714
K P PPP P PPP P G PPPP G A PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPA----PIMGGPPPPPPPPGVAGAGPPPPPPPPPAV 785
Query: 715 XXXGGXFF 738
G ++
Sbjct: 786 SAGGSRYY 793
Score = 29.5 bits (63), Expect = 0.67
Identities = 17/54 (31%), Positives = 18/54 (33%), Gaps = 5/54 (9%)
Frame = +1
Query: 409 KXPPPPXXXPLXP-----PXKKGPXXXXXXPPPPPXXXGGXXGGXXKKXPXXPP 555
K PPPP + P P P PPPP G G P PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/54 (33%), Positives = 19/54 (35%), Gaps = 2/54 (3%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPPXGXXXXGXXKKXXXXFXXXGGGKK--XPPPXXXP 437
PPP P G PP PPPP G G GG + P P P
Sbjct: 752 PPPAPIMGGPPP----PPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEP 801
Score = 27.9 bits (59), Expect = 2.1
Identities = 16/49 (32%), Positives = 16/49 (32%), Gaps = 1/49 (2%)
Frame = +1
Query: 283 PPPXPXXXGP-PRGXXXPPPPXGFXXGGXXKKXXAXFXXXGGXKXPPPP 426
PPP P P P P PP GG G PPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.6
Identities = 15/39 (38%), Positives = 15/39 (38%), Gaps = 2/39 (5%)
Frame = +1
Query: 253 PXXFXXXPXXPPPXP--XXXGPPRGXXXPPPPXGFXXGG 363
P P PPP P GPP PPPP GG
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPP---PPPPPPPAVSAGG 789
Score = 25.8 bits (54), Expect = 8.3
Identities = 14/51 (27%), Positives = 14/51 (27%)
Frame = +1
Query: 274 PXXPPPXPXXXGPPRGXXXPPPPXGFXXGGXXKKXXAXFXXXGGXKXPPPP 426
P PPP P PPP G G PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 30.7 bits (66), Expect = 0.29
Identities = 33/126 (26%), Positives = 34/126 (26%), Gaps = 5/126 (3%)
Frame = +3
Query: 294 PPXPGXPPGXXXPPPPXGXXXXGXXKKXXXXFXXXGGGKKXPPPXXXP--PXPPXKKGXQ 467
PP PG PPPP G G PP P PP +
Sbjct: 209 PPPMHHKPGEHMPPPPM-HHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHE 267
Query: 468 XXEXXXPPPPXKXGGXXGGGXXKXPXFXPPXGKXPPXXTXF---FFXPPPPXEXXPXXGX 638
E PPP G P P PP PPPP P G
Sbjct: 268 PGEHMPPPPMHHEPGEH---MPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEP--GE 322
Query: 639 GXXPPP 656
PPP
Sbjct: 323 HMPPPP 328
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.6
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPP 341
PP PP P PP PP P
Sbjct: 1717 PPSAPPMPAGPPSAPPPPLP 1736
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +1
Query: 415 PPPPXXXPLXPPXKKGPXXXXXXPPPP 495
PPPP P P P PPPP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPPXG 347
P P PP PP PP P G
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAG 1726
Score = 25.8 bits (54), Expect = 8.3
Identities = 12/43 (27%), Positives = 13/43 (30%)
Frame = +1
Query: 550 PPPXXKXPPXXLXFFFXPPPXGXXPPXXGXGGPPPPXRGXAXP 678
PP P PPP P PPPP + P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 8.3
Identities = 9/20 (45%), Positives = 9/20 (45%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPP 341
PPP PP PP PP
Sbjct: 206 PPPPPPQQNYPPAASSSAPP 225
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 25.8 bits (54), Expect = 8.3
Identities = 9/20 (45%), Positives = 9/20 (45%)
Frame = +3
Query: 282 PPPXPPXPGXPPGXXXPPPP 341
P P PP P P P PP
Sbjct: 3 PAPPPPPPAPAPAAAAPAPP 22
Score = 25.8 bits (54), Expect = 8.3
Identities = 25/107 (23%), Positives = 27/107 (25%), Gaps = 10/107 (9%)
Frame = +1
Query: 415 PPPPXXXPLXPPXKKGPXXXXXXPPPPPXXXGGXXGGXX-------KKXPXXPPPXXKXP 573
PP P P P P P GG G K PP P
Sbjct: 73 PPVPTGAPSLPTSSNNTQQAEERPSMPAL--GGLFAGGMPKLRHIGKSSASAAPPSAPAP 130
Query: 574 PXXLXFFFXP---PPXGXXPPXXGXGGPPPPXRGXAXPKKIKKXXPP 705
P P PP PP PP P + P + P
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 8.3
Identities = 25/94 (26%), Positives = 27/94 (28%), Gaps = 4/94 (4%)
Frame = +1
Query: 409 KXPPPPXXX----PLXPPXKKGPXXXXXXPPPPPXXXGGXXGGXXKKXPXXPPPXXKXPP 576
K PP P P+ P P PP P G P P P + P
Sbjct: 1020 KAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAP----PPVPAPSSEIPS 1075
Query: 577 XXLXFFFXPPPXGXXPPXXGXGGPPPPXRGXAXP 678
P P G P G PP P A P
Sbjct: 1076 I-------PAPSGAPPVPAPSGIPPVPKPSVAAP 1102
>SPBC11B10.08 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 204
Score = 22.6 bits (46), Expect(2) = 8.8
Identities = 9/20 (45%), Positives = 9/20 (45%)
Frame = +1
Query: 283 PPPXPXXXGPPRGXXXPPPP 342
P P P RG PPPP
Sbjct: 32 PKAKPQWECPVRGLTIPPPP 51
Score = 21.4 bits (43), Expect(2) = 8.8
Identities = 10/32 (31%), Positives = 10/32 (31%)
Frame = +1
Query: 403 GXKXPPPPXXXPLXPPXKKGPXXXXXXPPPPP 498
G PPPP PP P P P
Sbjct: 44 GLTIPPPPSVDHSAPPSGPPPSYSNSAAPATP 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.157 0.555
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,118,377
Number of Sequences: 5004
Number of extensions: 38771
Number of successful extensions: 245
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
- SilkBase 1999-2023 -