BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_M02
(983 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.020
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 0.93
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 1.0
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 3.1
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.1
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 3.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 5.4
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 5.4
SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo... 27 5.4
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 7.1
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 26 9.4
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.7 bits (76), Expect = 0.020
Identities = 15/34 (44%), Positives = 15/34 (44%)
Frame = -1
Query: 650 PSXXXXPPHPXGAPXXPPXXSAPXRPPPXXFPXP 549
PS PP P G P PP A PPP P P
Sbjct: 451 PSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 30.3 bits (65), Expect = 0.44
Identities = 16/40 (40%), Positives = 17/40 (42%), Gaps = 2/40 (5%)
Frame = -1
Query: 632 PPHPXGAPXXPPXXSA-PXRPP-PXXFPXPXXGPPPGXXA 519
PP P AP PP + P PP P P P P P A
Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAA 486
Score = 29.9 bits (64), Expect = 0.58
Identities = 19/51 (37%), Positives = 19/51 (37%), Gaps = 2/51 (3%)
Frame = -1
Query: 632 PPHPXGAPXXPP-XXSAPXRPP-PXXFPXPXXGPPPGXXAXGXPXXXXRAP 486
P P GAP PP SAP PP P P PP P AP
Sbjct: 437 PSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 29.1 bits (62), Expect = 1.0
Identities = 49/227 (21%), Positives = 59/227 (25%), Gaps = 4/227 (1%)
Frame = -3
Query: 975 PPXPRXRLXTXPPEXLPXXXXP---PLXPXXYXPSXXPXXRPXLXXXXXLXXXDXTXXXX 805
PP P + PPE +P P P+ P P P
Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSK 289
Query: 804 TXGXXPREXXXXXXSXASXXRPPSTXXXXPRXXG-PXGXXGVXXALXPPXXXPXXVXXPP 628
P A+ RPP R G P G + PP PP
Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPP---------PP 340
Query: 627 PPXXGXSXXAAXXRPXAAPSPXFPXPXQXPXPXXXGXGXPXXXXARAXXXXRGGXPLPPX 448
PP + + P +P P P P G P +RA P PP
Sbjct: 341 PPPRSNAAGSIPLPPQGRSAPPPPPPRSAP---STGRQPPPLSSSRAV----SNPPAPP- 392
Query: 447 LPXXXAXXXXXXPPXXSAXXXRXXPEXPXXXSAXXXXXXKXPXXGPP 307
P PP +A P P S P PP
Sbjct: 393 -PAIPGRSAPALPPLGNA-SRTSTPPVPTPPSLPPSAPPSLPPSAPP 437
Score = 28.7 bits (61), Expect = 1.3
Identities = 15/37 (40%), Positives = 15/37 (40%), Gaps = 3/37 (8%)
Frame = -1
Query: 632 PPHPXGA---PXXPPXXSAPXRPPPXXFPXPXXGPPP 531
PP A P P SAP PPP P PPP
Sbjct: 342 PPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
Score = 26.2 bits (55), Expect = 7.1
Identities = 15/48 (31%), Positives = 16/48 (33%)
Frame = -1
Query: 650 PSXXXXPPHPXGAPXXPPXXSAPXRPPPXXFPXPXXGPPPGXXAXGXP 507
P+ PP P P P P R P P P PPP P
Sbjct: 224 PTSTSAPPIPPSIPSSRP----PERVPSLSAPAPPPIPPPSNGTVSSP 267
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect(2) = 0.93
Identities = 16/48 (33%), Positives = 17/48 (35%)
Frame = -1
Query: 623 PXGAPXXPPXXSAPXRPPPXXFPXPXXGPPPGXXAXGXPXXXXRAPXS 480
P AP PP + RPP P P PP A P P S
Sbjct: 124 PPSAPA-PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSS 170
Score = 21.0 bits (42), Expect(2) = 0.93
Identities = 8/17 (47%), Positives = 8/17 (47%)
Frame = -1
Query: 650 PSXXXXPPHPXGAPXXP 600
P PP P GAP P
Sbjct: 67 PKSFAAPPVPTGAPSLP 83
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 1.0
Identities = 15/49 (30%), Positives = 17/49 (34%)
Frame = -1
Query: 632 PPHPXGAPXXPPXXSAPXRPPPXXFPXPXXGPPPGXXAXGXPXXXXRAP 486
P HP P P +AP + P P PP A P AP
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAP 1731
Score = 26.6 bits (56), Expect = 5.4
Identities = 14/43 (32%), Positives = 14/43 (32%), Gaps = 1/43 (2%)
Frame = -1
Query: 632 PPHPXGAPXXPPXXSAPXRPPPXXFPX-PXXGPPPGXXAXGXP 507
PP PP S P P P P PPP A P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.5 bits (58), Expect = 3.1
Identities = 17/47 (36%), Positives = 18/47 (38%), Gaps = 4/47 (8%)
Frame = -1
Query: 614 APXXPPXXSAPXRPPPXXFP---XPXXGPPPGXXA-XGXPXXXXRAP 486
AP PP + P PP P P GPP G A G P P
Sbjct: 1881 APPPPPPMALPKAGPPSAAPTSALPPAGPPAGATAISGNPGMPAPVP 1927
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.5 bits (58), Expect = 3.1
Identities = 19/54 (35%), Positives = 20/54 (37%), Gaps = 6/54 (11%)
Frame = -1
Query: 650 PSXXXXP-PHPX-GAPXXPPXXSAPXRPPP----XXFPXPXXGPPPGXXAXGXP 507
PS P P P G P PP AP P P P P PP + G P
Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLP 1227
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 3.1
Identities = 17/45 (37%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = -1
Query: 632 PPHPXGAPXXPPXXSAPXR-PPPXXFPX--PXXGPPPGXXAXGXP 507
PP P A P AP PPP P PPPG G P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP 776
Score = 26.6 bits (56), Expect = 5.4
Identities = 15/44 (34%), Positives = 15/44 (34%), Gaps = 5/44 (11%)
Frame = -1
Query: 629 PHPXGAPXXPPXXSAPXRPPP-----XXFPXPXXGPPPGXXAXG 513
P P P P P PPP P P PPP A G
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 25.8 bits (54), Expect = 9.4
Identities = 14/41 (34%), Positives = 15/41 (36%), Gaps = 1/41 (2%)
Frame = -1
Query: 650 PSXXXXPPHPXGAPXXPPXXSAPXRPPPXXFP-XPXXGPPP 531
P+ P P P PP PPP P GPPP
Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = -1
Query: 599 PXXSAPXRPPPXXFPXPXXGPPP 531
P + P PPP F P PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPP 27
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 5.4
Identities = 17/43 (39%), Positives = 18/43 (41%), Gaps = 3/43 (6%)
Frame = +1
Query: 532 GGGP--LXGXGKXXGGGRXG-ALXXGGXXGAPXGWGGXXXXXG 651
GGGP G GGG G GG G P G+GG G
Sbjct: 227 GGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHG 269
Score = 25.8 bits (54), Expect = 9.4
Identities = 14/40 (35%), Positives = 15/40 (37%)
Frame = +1
Query: 532 GGGPLXGXGKXXGGGRXGALXXGGXXGAPXGWGGXXXXXG 651
G G G G GG GG G P G+GG G
Sbjct: 209 GFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFG 248
>SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 26.6 bits (56), Expect = 5.4
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -1
Query: 587 APXRPPPXXFPXPXXGPPP 531
AP PPP F GPPP
Sbjct: 347 APGAPPPWLFAMQRYGPPP 365
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.2 bits (55), Expect = 7.1
Identities = 12/33 (36%), Positives = 12/33 (36%)
Frame = -1
Query: 629 PHPXGAPXXPPXXSAPXRPPPXXFPXPXXGPPP 531
P P AP P S P RP P P P
Sbjct: 635 PQPPAAPVVPEVPSVPQRPAVPVVPEAPSVPQP 667
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 25.8 bits (54), Expect = 9.4
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = -2
Query: 982 RAPPXXPXKVXHRPPRATS 926
R PP P V H PPR S
Sbjct: 224 RGPPSPPPPVLHSPPRKVS 242
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.143 0.484
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,385,270
Number of Sequences: 5004
Number of extensions: 14003
Number of successful extensions: 77
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 61
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 507285244
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
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