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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_J13
         (954 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    28   2.2  
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce...    27   3.9  
SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr ...    26   6.8  
SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyce...    26   9.0  

>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 27.9 bits (59), Expect = 2.2
 Identities = 16/71 (22%), Positives = 34/71 (47%)
 Frame = +3

Query: 438 VDENGGTEQSILTTVESIENSGIDLQNINPYATEVHFDETHFEISQSNKLTFPLENWTDE 617
           V E+G +  SI +T  S   S + +   +  ++ VH    H   S+S+ ++ P    +  
Sbjct: 596 VSESGSSSASITSTYPS---STLSMTTSHLSSSSVHSSSAHSSSSRSSSMSLPPSAGSST 652

Query: 618 EIQRLLVFYMY 650
            +QR+ +  ++
Sbjct: 653 SLQRISLLCVF 663


>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1610

 Score = 27.1 bits (57), Expect = 3.9
 Identities = 10/30 (33%), Positives = 20/30 (66%)
 Frame = -2

Query: 326  LKRXDNFALQIVKKKTKNYKINEXLRVCIL 237
            +K  + FAL++    +KNY++N  ++ CI+
Sbjct: 1161 VKSFELFALRLYNALSKNYEMNSDIKDCIV 1190


>SPBC713.06 |adl1|lig3|DNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 774

 Score = 26.2 bits (55), Expect = 6.8
 Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
 Frame = +3

Query: 156 CSGKNAXQMPVL-KFXFFCCHFNLI 227
           C GK+  QMP++ +   F  HFNLI
Sbjct: 529 CDGKSCLQMPLIERRRMFFEHFNLI 553


>SPCC1235.02 |bio2|SPCC320.01c|biotin synthase|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 363

 Score = 25.8 bits (54), Expect = 9.0
 Identities = 10/23 (43%), Positives = 16/23 (69%)
 Frame = +3

Query: 564 EISQSNKLTFPLENWTDEEIQRL 632
           +I +S+ L+    NWT EEIQ++
Sbjct: 9   QIRRSSALSLVRNNWTREEIQKI 31


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,465,419
Number of Sequences: 5004
Number of extensions: 44570
Number of successful extensions: 129
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 487313384
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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