Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_J07
(915 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.30
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.70
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 29 1.2
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 27 2.8
SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 27 2.8
SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 27 3.7
SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 27 4.9
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 26 6.5
SPAC3A11.04 |||siepin homolog|Schizosaccharomyces pombe|chr 1|||... 26 6.5
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 8.6
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 26 8.6
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 30.7 bits (66), Expect = 0.30
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +3
Query: 498 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 674
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVP 360
Query: 675 SNTTGT 692
+T +
Sbjct: 361 PTSTSS 366
Score = 30.7 bits (66), Expect = 0.30
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +3
Query: 498 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTV-IAPLTP 674
VTP P S S PP +T T S P +++ S T + T +ST + P P
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414
Query: 675 SNTTGT 692
++ T
Sbjct: 415 PTSSST 420
Score = 29.9 bits (64), Expect = 0.53
Identities = 23/64 (35%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +3
Query: 504 PKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTPSN 680
P T + S P P T+ TS S PP NST + +T V + SST P P++
Sbjct: 264 PPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNST-TPVTPTVPPTSTSSTSTPP-PPAS 321
Query: 681 TTGT 692
T+ T
Sbjct: 322 TSST 325
Score = 29.5 bits (63), Expect = 0.70
Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Frame = +3
Query: 498 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 674
+TP P S S+P P T+ + S P T ST + + + S+T + P P
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306
Query: 675 SNTTGT 692
+T +
Sbjct: 307 PTSTSS 312
Score = 29.1 bits (62), Expect = 0.92
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +3
Query: 498 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAPLTP 674
+TP P S S+P P T+ + S P T ST S T + SS++ P+TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249
Query: 675 S 677
+
Sbjct: 250 T 250
Score = 28.3 bits (60), Expect = 1.6
Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +3
Query: 489 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLVMTVSSTVIAP 665
L++ TP T P S S W TT T S T+ST +T T ++ +
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657
Query: 666 LTPSNTTGT 692
T S++T T
Sbjct: 658 STSSSSTAT 666
>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 29.5 bits (63), Expect = 0.70
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = -3
Query: 580 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 479
DLE N +V Q+ G G+FL G FG + FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -3
Query: 712 FVHGGLKVPVVFEGVSGAITVDDTVIT 632
F H + V G+ GAIT+DD++IT
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSIIT 133
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +2
Query: 191 QLYMSVVIGEY-EXAIAKCSEYLKEKKGEVIKEAVKRLIE 307
Q+++++V+ Y A+ EYL+EK I + K ++E
Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595
>SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 417
Score = 27.5 bits (58), Expect = 2.8
Identities = 18/91 (19%), Positives = 36/91 (39%)
Frame = -1
Query: 327 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAXSYSPMTTLIYSCSASTSSVLGASVAL 148
M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++
Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57
Query: 147 EASAHTARTKANKVRXXLGAVVSFESKATNN 55
+A A T A S A+++
Sbjct: 58 NGAAKEAATAAGDAETTSSVAASVTPAASSS 88
>SPCC576.13 |swc5||chromatin remodeling complex subunit
Swc5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 215
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 257 KEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 367
K +K + + +++++ K NT++ A Q W+K KE
Sbjct: 139 KRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175
>SPAC3C7.09 |set8||lysine methyltransferase Set8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 429
Score = 26.6 bits (56), Expect = 4.9
Identities = 33/121 (27%), Positives = 46/121 (38%), Gaps = 4/121 (3%)
Frame = +2
Query: 458 KLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRV--YFKIMSTEDKQYLKLDNTKGS 631
K ID+ I +G K S+ F P E +R+ K++ ED LD K
Sbjct: 219 KDIDENEEVTINYGSEKGSAEFLFSYGFLPEPEGDRITNVMKLLIPEDSND-SLDLAKRR 277
Query: 632 SDDRIIYGDSTADTFKHHWYLEPSMYESDVMFFVYNRE--YNSVMTLDEDMAANEDRETL 805
S + +D+ W+ P ++ F V N E N M DE A D E
Sbjct: 278 SCKTPPMIEFVSDSSGELWWHAPFLF-----FSVLNVEDFTNFKMVCDESKAQTVDWEFE 332
Query: 806 G 808
G
Sbjct: 333 G 333
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 26.2 bits (55), Expect = 6.5
Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 6/51 (11%)
Frame = +2
Query: 632 SDDRIIYGDSTADTFKHHWYLEP----SMYESDVMFFVYNR--EYNSVMTL 766
SDDR+ GD+ + +Y++ +MYE ++ Y YN V+ L
Sbjct: 84 SDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLAL 134
>SPAC3A11.04 |||siepin homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 236
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = -2
Query: 809 APRFHGLRWRPYLHQVS 759
A + HG+RW Y H+VS
Sbjct: 185 ASKLHGMRWFMYTHKVS 201
>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
Smc6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1140
Score = 25.8 bits (54), Expect = 8.6
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +2
Query: 428 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 520
L+ K+D A K+ DQ H K+ +DK S
Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 25.8 bits (54), Expect = 8.6
Identities = 22/81 (27%), Positives = 36/81 (44%), Gaps = 2/81 (2%)
Frame = -1
Query: 288 ASLITSPFFSFRYSEHLAIAXSYSPMTTLIYSCSASTSSVLGASVALEA--SAHTARTKA 115
+S ITS + + ++A S + TT S++TSS L ++ A + S+ A +
Sbjct: 141 SSSITSSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSATSSSLASSSL 200
Query: 114 NKVRXXLGAVVSFESKATNNS 52
N S S A +NS
Sbjct: 201 NSTTSATATSSSLSSTAASNS 221
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,299,756
Number of Sequences: 5004
Number of extensions: 70276
Number of successful extensions: 271
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 238
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 269
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -