Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_I19
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr ... 50 6e-07
SPAC23C4.06c |||methyltransferase |Schizosaccharomyces pombe|chr... 42 2e-04
SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces p... 39 0.001
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 33 0.041
SPCC4G3.16 |||CMP/dCMP deaminase family|Schizosaccharomyces pomb... 33 0.055
SPAC25B8.10 |||trans-aconitate 3-methyltransferase |Schizosaccha... 32 0.13
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 31 0.22
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 29 0.89
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 27 2.7
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 27 3.6
SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease XPF... 26 8.3
>SPAC3A11.03 |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 247
Score = 49.6 bits (113), Expect = 6e-07
Identities = 39/138 (28%), Positives = 64/138 (46%), Gaps = 1/138 (0%)
Frame = +1
Query: 364 SRIFLKKLINCIEPVQEVHDNLYAELCRAMNNSAIEDYCYRHYVISNDLNNIIIMKETKN 543
+R FLK ++ + H+ L S Y + + I+++E+++
Sbjct: 83 TRAFLKSYFRFLDSIDSGHNERNEALLYTYIESLSSTYIPPVQYSLGEYD--ILIRESRH 140
Query: 544 MVVN-GTTGMRTWEAALMLSDWILCNKELFSSKDVLELGSGIGFTGITLAKFCEPKSVTM 720
+++ GTTG RTWEA + L+++I + S VLELG+G G I AK V
Sbjct: 141 VLLREGTTGARTWEAGMALAEYIY-QHPVQSGMRVLELGAGTGLVSILCAKM--GSIVLA 197
Query: 721 TDCHEEVLXVLCENVDIN 774
TD +V + EN +N
Sbjct: 198 TDGDTKVCDGVRENARLN 215
>SPAC23C4.06c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 327
Score = 41.5 bits (93), Expect = 2e-04
Identities = 30/82 (36%), Positives = 45/82 (54%), Gaps = 5/82 (6%)
Frame = +1
Query: 577 WEAALMLSDWILCNKELFS---SKD--VLELGSGIGFTGITLAKFCEPKSVTMTDCHEEV 741
W+A ++ S IL + +S KD VLELGSG G GI++A V+MTD E+
Sbjct: 159 WDAGVVFSKKILSDDWHYSFSNRKDINVLELGSGCGIVGISIASKYPRALVSMTDT-EDA 217
Query: 742 LXVLCENVDINFPSQCKNRSSD 807
+ + +NV+ N + N +SD
Sbjct: 218 IEFMEKNVEKNKSAMSNNITSD 239
>SPCC338.11c |rrg1|uvi22|methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 303
Score = 38.7 bits (86), Expect = 0.001
Identities = 29/98 (29%), Positives = 52/98 (53%), Gaps = 3/98 (3%)
Frame = +1
Query: 406 VQEVHDNLYAELCRAM---NNSAIEDYCYRHYVISNDLNNIIIMKETKNMVVNGTTGMRT 576
V+E D L + +C + + R + +SN ++ ++++E M N T G +T
Sbjct: 61 VEEERDYLVSTICERIAERSGRLAAPTRKREFSLSNGVS--VVLREP-TMTYN-TLGFKT 116
Query: 577 WEAALMLSDWILCNKELFSSKDVLELGSGIGFTGITLA 690
W +A +LS + ++L +S + LELG+G G GI+ A
Sbjct: 117 WGSAPLLSANLPKWEDLSNSINALELGAGTGLVGISAA 154
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 33.5 bits (73), Expect = 0.041
Identities = 20/56 (35%), Positives = 29/56 (51%)
Frame = +1
Query: 577 WEAALMLSDWILCNKELFSSKDVLELGSGIGFTGITLAKFCEPKSVTMTDCHEEVL 744
W + + L+++I N + +K VLELG+G G I A F K V TD + L
Sbjct: 58 WNSGIELANYIDKNPDTVRAKKVLELGAGAGLPSIVSA-FDGAKFVVSTDYPDPAL 112
>SPCC4G3.16 |||CMP/dCMP deaminase family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 405
Score = 33.1 bits (72), Expect = 0.055
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 5/52 (9%)
Frame = +1
Query: 556 GTTGMRTWEAALMLSDWILCNKELFSS----KDVLELGSGI-GFTGITLAKF 696
G+TG W+ ++ + W+L +S +LELGSGI G GI L+ F
Sbjct: 65 GSTGSVLWKTSVKVVPWLLQQSWFMNSLTPKTSILELGSGISGLAGILLSPF 116
>SPAC25B8.10 |||trans-aconitate 3-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 256
Score = 31.9 bits (69), Expect = 0.13
Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 1/59 (1%)
Frame = +1
Query: 580 EAALMLSDWILCNKELFSSKDVLELGSGIG-FTGITLAKFCEPKSVTMTDCHEEVLXVL 753
E + ++DWI + + +LELG+G G FT +A PK + D + E+L VL
Sbjct: 23 EYPIGITDWITDEFLIDETSIILELGAGTGKFTPRIIAS--HPKEIIAVDVYPEMLDVL 79
>SPAC23H4.09 |cdb4||curved DNA-binding protein
Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 31.1 bits (67), Expect = 0.22
Identities = 14/43 (32%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +1
Query: 298 ELFVDLTLRSP-VIKKYPISTELSRIFLKKLINCIEPVQEVHD 423
E VD +L +P + KY I+ E+S+ +KK++ +P +++D
Sbjct: 9 ETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYD 51
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 29.1 bits (62), Expect = 0.89
Identities = 24/82 (29%), Positives = 36/82 (43%), Gaps = 7/82 (8%)
Frame = +1
Query: 487 HYVISNDLNNIIIMKETKNMVVNGTTGMRTWEAALMLSD-W---ILCNK--ELFSSK-DV 645
H+ I + L I + ++ +V+ T L +D W LC K L S K D+
Sbjct: 324 HFFIRDSLEVPFIWQHRRDYIVHNNRERNTITPLLSQNDLWNIFFLCTKFWSLHSKKQDI 383
Query: 646 LELGSGIGFTGITLAKFCEPKS 711
L+L S +G + FCE S
Sbjct: 384 LKLYSDLGINDDLVVPFCEAAS 405
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 27.5 bits (58), Expect = 2.7
Identities = 21/76 (27%), Positives = 31/76 (40%), Gaps = 3/76 (3%)
Frame = +1
Query: 367 RIFLKKLINCIEPVQEVHDNLYAELCRAMNNSAIEDYCYRHYVISNDLNNII---IMKET 537
RI K L C+E V++V + Y L NN E C V N + ++
Sbjct: 435 RILQKPL--CLEDVKDVDEVYYESLKWIKNNDVDESLCLNFSVEENRFGESVTVDLIPNG 492
Query: 538 KNMVVNGTTGMRTWEA 585
+N+ VN M +A
Sbjct: 493 RNIAVNNQNKMNYLKA 508
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 27.1 bits (57), Expect = 3.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +1
Query: 445 RAMNNSAIEDYCYRHYVISNDLNNIIIM 528
+ NNS E YRH + D+NN +IM
Sbjct: 585 QVFNNSPDETAFYRHMLNHEDVNNSLIM 612
>SPCC970.01 |rad16|rad10, rad20, swi9|DNA repair endonuclease
XPF|Schizosaccharomyces pombe|chr 3|||Manual
Length = 892
Score = 25.8 bits (54), Expect = 8.3
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -1
Query: 823 IHMLXHQSFCFYIVMENLYPHFHTVLVEPL 734
I+ L H C ++ +YP FH V+ E L
Sbjct: 168 INALSHCLRCLFLRHVFIYPRFHVVVAESL 197
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,030,723
Number of Sequences: 5004
Number of extensions: 56430
Number of successful extensions: 172
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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