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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_G03
         (923 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    29   0.70 
SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom...    28   1.6  
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces...    27   4.9  
SPAC664.07c |rad9||checkpoint clamp complex protein Rad9|Schizos...    26   6.5  
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   6.5  

>SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 515

 Score = 29.5 bits (63), Expect = 0.70
 Identities = 13/38 (34%), Positives = 23/38 (60%)
 Frame = -1

Query: 602 VNLSFEDFLVLVEVVQIYGSIESQHYHLRCFCRVEAAR 489
           + LS  D   ++ ++QI  +I+  H  L+CFC+V  A+
Sbjct: 292 ITLSSSDVFTIIHLLQIPDNIK--HIELQCFCKVIVAQ 327


>SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 437

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 10/17 (58%), Positives = 14/17 (82%)
 Frame = +1

Query: 754 EWAGEMIGMREILKKLR 804
           +WAGE++GM E LK +R
Sbjct: 344 QWAGEVVGMSERLKSMR 360


>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 879

 Score = 26.6 bits (56), Expect = 4.9
 Identities = 27/85 (31%), Positives = 38/85 (44%), Gaps = 7/85 (8%)
 Frame = +1

Query: 112 KNYDQFASTPXFVCF*STVHINRKIRRKSSL---FVPK--EHRVMGISP--TQPPADVFI 270
           +N +Q   TP +  F S +H N + ++K  L   FV K  ++    I P   Q  A+   
Sbjct: 550 ENVEQEVETPVWETFSSLLHANARTKKKELLNINFVRKYIQYAKSRIHPILNQATAEYIT 609

Query: 271 NVSTGTRI*TDVLQDYILMTSANTA 345
           N+  G R   D LQ     TS  TA
Sbjct: 610 NIYCGLR--NDDLQGNQRRTSPLTA 632


>SPAC664.07c |rad9||checkpoint clamp complex protein
           Rad9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 426

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 14/51 (27%), Positives = 26/51 (50%)
 Frame = +3

Query: 438 AECQLPYCFCSKDGTLIPGGLDPAETPQMIMLTFDGAVNLNNFDKYKKVFK 590
           +EC++ + F  K G +    +   +T Q +   FD +++ NNF    K+ K
Sbjct: 134 SECRIIFKFLCKHGVIKTYKISYEQT-QTLHAVFDKSLSHNNFQINSKILK 183


>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 486

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 9/31 (29%), Positives = 17/31 (54%)
 Frame = -1

Query: 776 PIISPAHSSYPLSCKPCCCETVPVAISCPSC 684
           P++SP   ++   C PC  + +  +  CP+C
Sbjct: 179 PVVSPCGHTF---CGPCLMQALTQSPQCPTC 206


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,929,418
Number of Sequences: 5004
Number of extensions: 87367
Number of successful extensions: 262
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 262
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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