Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_F15
(915 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 241 7e-65
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 142 5e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 140 3e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 138 8e-34
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 4.9
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 8.6
SPBC216.04c |||methionine sulfoxide |Schizosaccharomyces pombe|c... 26 8.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 241 bits (591), Expect = 7e-65
Identities = 103/174 (59%), Positives = 132/174 (75%)
Frame = +2
Query: 221 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGDSDLQLERINVYYNEASAGKYV 400
MREI+++Q G CGNQ+G FW I+DEHG+D +G YHG S+ Q ER+NVY+NEA+ GKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 401 PRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWAKGHYTEGVEILESALDVI 580
PR VL+DL+P TMDAV+SG FG LFRPDN +YGQ+ A N WAKGHYTEG E+ ++ LDV+
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 581 RREAEGCDCLQGFQMSHXXXXXXXXXXXXXXVNRIREEYPDRIILTFSVFXSPE 742
RREAE CD LQGFQ++H +++IREEYPDR++ TFSV +P+
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
Score = 42.3 bits (95), Expect = 9e-05
Identities = 28/74 (37%), Positives = 39/74 (52%), Gaps = 5/74 (6%)
Frame = +1
Query: 700 RQNYTD----LFSIXKS-RVSDCVVEPYNTTLAVNQLARILITRSVSITKHCTXICFKTL 864
R+ Y D FS+ + + SD VVEPYN TL+++QL + + I TL
Sbjct: 156 REEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTL 215
Query: 865 KLTXPTYGDLNPLV 906
K+ P+Y DLN LV
Sbjct: 216 KIKSPSYDDLNHLV 229
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 142 bits (345), Expect = 5e-35
Identities = 68/176 (38%), Positives = 103/176 (58%), Gaps = 2/176 (1%)
Frame = +2
Query: 221 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGDSDLQLER--INVYYNEASAGK 394
MREII++ VG G QIG WE+ EHGI P+G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 395 YVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWAKGHYTEGVEILESALD 574
YVPR++ +DL+P +D VR+GP+ LF P+ + G+ A+NN+A+GHYT G E+++ D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 575 VIRREAEGCDCLQGFQMSHXXXXXXXXXXXXXXVNRIREEYPDRIILTFSVFXSPE 742
IRR A+ C LQGF + H + R+ EY + L FSV+ +P+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQ 176
Score = 37.1 bits (82), Expect = 0.003
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = +1
Query: 721 FSIXKS-RVSDCVVEPYNTTLAVNQLARILITRSVSITKHCTXICFKTLKLTXPTYGDLN 897
FS+ + +VS VVEPYN+ L + + + + C IC + L + P+Y +LN
Sbjct: 169 FSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLN 228
Query: 898 PLV 906
L+
Sbjct: 229 RLI 231
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 140 bits (339), Expect = 3e-34
Identities = 66/175 (37%), Positives = 102/175 (58%), Gaps = 2/175 (1%)
Frame = +2
Query: 224 REIINLQVGSCGNQIGGKFWEVISDEHGIDPSGCYHGDSDLQLERINVYYNEASAGKYVP 403
REII LQ G CGNQIG +FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 404 RTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQN--CAANNWAKGHYTEGVEILESALDV 577
R +LIDL+P ++ + S +G L+ P+N + +N A NNWA G Y+ I E +D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 578 IRREAEGCDCLQGFQMSHXXXXXXXXXXXXXXVNRIREEYPDRIILTFSVFXSPE 742
I REA+G D L+GF + H + R+ + YP +II T+SVF + +
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
Score = 29.1 bits (62), Expect = 0.92
Identities = 19/55 (34%), Positives = 25/55 (45%)
Frame = +1
Query: 742 VSDCVVEPYNTTLAVNQLARILITRSVSITKHCTXICFKTLKLTXPTYGDLNPLV 906
VSD VV+PYN+ LA+ +L + V I L PT+ N LV
Sbjct: 178 VSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLV 232
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 138 bits (335), Expect = 8e-34
Identities = 68/180 (37%), Positives = 103/180 (57%), Gaps = 6/180 (3%)
Frame = +2
Query: 221 MREIINLQVGSCGNQIGGKFWEVISDEHGIDPSGC------YHGDSDLQLERINVYYNEA 382
MRE+I++ VG G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 383 SAGKYVPRTVLIDLKPATMDAVRSGPFGCLFRPDNFVYGQNCAANNWAKGHYTEGVEILE 562
GK+VPR++ +DL+P +D VR+GP+ LF P+ V G+ A+NN+A+GHYT G E+++
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 563 SALDVIRREAEGCDCLQGFQMSHXXXXXXXXXXXXXXVNRIREEYPDRIILTFSVFXSPE 742
S L+ IRR A+ C LQGF + H + R+ EY + L FSV+ +P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Score = 35.9 bits (79), Expect = 0.008
Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +1
Query: 721 FSIXKS-RVSDCVVEPYNTTLAVNQLARILITRSVSITKHCTXICFKTLKLTXPTYGDLN 897
FS+ + +VS VVEPYN+ L + + + C IC + L + PTY +LN
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLN 232
Query: 898 PLV 906
L+
Sbjct: 233 RLI 235
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +2
Query: 524 AKGHYTEGVEILESALDVIRREAEGCDCLQ 613
A+GH GVE++ + D +R+++E L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.8 bits (54), Expect = 8.6
Identities = 15/30 (50%), Positives = 18/30 (60%), Gaps = 3/30 (10%)
Frame = +3
Query: 297 TSTASIQADATTATPTCN---SKGSTCTTT 377
TST S+ +TTAT +C S ST TTT
Sbjct: 757 TSTGSVTTTSTTATASCTLPISYTSTPTTT 786
>SPBC216.04c |||methionine sulfoxide |Schizosaccharomyces pombe|chr
2|||Manual
Length = 138
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = +3
Query: 753 CGGAI*HNAGGQSVGENTDHXFCLDNEALYXH 848
CGG + H G+ TD C+++ +L H
Sbjct: 100 CGGHLGHIFKGEGYSNPTDERHCINSASLEFH 131
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,475,618
Number of Sequences: 5004
Number of extensions: 70012
Number of successful extensions: 180
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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