BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_F12
(921 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 29 1.2
SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces po... 29 1.2
SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 28 1.6
SPAC9G1.10c |||inositol polyphosphate phosphatase |Schizosacchar... 26 6.5
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 26 8.6
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 28.7 bits (61), Expect = 1.2
Identities = 19/76 (25%), Positives = 38/76 (50%), Gaps = 1/76 (1%)
Frame = +1
Query: 283 LYLLAELKT-ISLKVTTVDMQKPPPDFRTNFEATHPPILIDNGLAILENEKIERHIMKSV 459
+++L ELK +KV + PP + PI++D+G+ +E+ I H+++
Sbjct: 16 VWMLEELKVPYEIKVYDRVDGRAPPAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRK- 74
Query: 460 PXGHNLFVQDKEVASL 507
G + +++VA L
Sbjct: 75 -YGPSFKPSEEDVAEL 89
>SPAC4A8.07c |||sphingoid long chain base |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 458
Score = 28.7 bits (61), Expect = 1.2
Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +1
Query: 481 VQDKEVASLIENLYSKLKLVLVR-KDEQKSAALRAHLGRIDGLLERRGD 624
+ + E + + +S ++VL R KD KS A +G DG+L GD
Sbjct: 124 IWESEAEPVFSSAHSICEVVLTRRKDHAKSIAKNLDVGSYDGILSVGGD 172
>SPAC1039.03 |||esterase/lipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 341
Score = 28.3 bits (60), Expect = 1.6
Identities = 20/55 (36%), Positives = 24/55 (43%), Gaps = 3/55 (5%)
Frame = -3
Query: 631 EPGLPCVLEVHR--CVRDELVTP-LISVHLCEPAPASVLNTGSRLETRPPCPARI 476
E G PC L H V + T + H+CE A V+N RL P PA I
Sbjct: 97 EGGWPCFLWFHGGGWVLGNINTENSFATHMCEQAKCVVVNVDYRLAPEDPFPACI 151
>SPAC9G1.10c |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1191
Score = 26.2 bits (55), Expect = 6.5
Identities = 18/43 (41%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
Frame = -3
Query: 529 VLNTGSR-LETRPPCPARIGCVPLARTS*CDVRSFRSQVSPAH 404
VLN G R LET PP P+ P+A + R+ SQ P H
Sbjct: 258 VLNIGDRSLETPPPIPSPRPPQPVAVEAIQQSRAVISQQLPLH 300
>SPAC23D3.13c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1616
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/39 (33%), Positives = 22/39 (56%)
Frame = +1
Query: 427 EKIERHIMKSVPXGHNLFVQDKEVASLIENLYSKLKLVL 543
+KIERHI S+ L + D ++E+L++ L V+
Sbjct: 67 KKIERHIYISLNSIQLLAINDALSPDILESLFNSLNAVI 105
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,556,770
Number of Sequences: 5004
Number of extensions: 72530
Number of successful extensions: 199
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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