Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_E18
(892 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 275 5e-75
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 273 3e-74
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 62 8e-11
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 48 2e-06
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 39 0.001
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 32 0.13
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 2.1
SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr... 27 3.6
SPCC1020.05 |||phosphoprotein phosphatase |Schizosaccharomyces p... 27 4.7
SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2 |S... 26 8.3
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 275 bits (675), Expect = 5e-75
Identities = 132/262 (50%), Positives = 168/262 (64%)
Frame = +3
Query: 81 MSTVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPE 260
MS GK I C AAVAW A +PLSIE+I+V PPKA EVRVK+ + VCHTDAYTLSG DPE
Sbjct: 1 MSFEGKTITCKAAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLSGVDPE 60
Query: 261 GVFPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQ 440
G FP+VL +V+PGDHV+ LY P+C CKFC + KTNLC K+R TQ
Sbjct: 61 GAFPIVLGHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQ 120
Query: 441 GQGVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVSLLGCGVPT 620
G+G+MPDGT RF C+ + L H+MGCS+FSQYTVV +ISL ++ +APL + LLGCGV T
Sbjct: 121 GRGLMPDGTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTT 180
Query: 621 GYGAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKKLESM 800
G+GA ++AKVE GS A+ +RII +DIN DK AKK +
Sbjct: 181 GFGAVTHSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGAT 240
Query: 801 NLSTLRIMINXSPVLVDLTDGG 866
+ + + ++D+TDGG
Sbjct: 241 DFIDSSKVKDLVQYVIDVTDGG 262
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 273 bits (669), Expect = 3e-74
Identities = 128/261 (49%), Positives = 165/261 (63%), Gaps = 1/261 (0%)
Frame = +3
Query: 87 TVGKVIKCLAAVAWEAGKPLSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGV 266
T GK+I C AAVAW+ PLSIE ++V PP+ EVR+KI +GVCHTDAYTLSGKDPEG+
Sbjct: 6 TAGKIINCKAAVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLSGKDPEGL 65
Query: 267 FPVVLXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQ 446
FPV+L T+V+ GD V+ LY P+C TCKFC + KTNLC ++R+TQG+
Sbjct: 66 FPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIRTTQGK 125
Query: 447 GVMPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVSLLGCGVPTGY 626
G+MPDGT RF C G L HFMGCSTFS+YTVV +IS+ + APLD V LLGCG+ TGY
Sbjct: 126 GLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCGITTGY 185
Query: 627 GAALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKKLESMN- 803
GAA TA ++ G + A+F RI G+D+NP+K A + +
Sbjct: 186 GAATITADIKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSFGATDF 245
Query: 804 LSTLRIMINXSPVLVDLTDGG 866
++ + VL+ TDGG
Sbjct: 246 INPNDLQSPIQDVLIHETDGG 266
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 62.5 bits (145), Expect = 8e-11
Identities = 62/231 (26%), Positives = 86/231 (37%), Gaps = 4/231 (1%)
Frame = +3
Query: 105 KCLAAVAWEAGKPLSI--EEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDP-EGVFPV 275
K LAAV G P ++ EE+ V P EV V I TGVCHTD + L G P P+
Sbjct: 6 KQLAAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKMPL 65
Query: 276 VLXXXXXXXXXXXXXXXTSVKPGDHV-VPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGV 452
+ T +K GD V V C C++C+ + +C + Q G
Sbjct: 66 IGGHEGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHI---QLSGY 122
Query: 453 MPDGTRRFRCKGQELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVSLLGCGVPTGYGA 632
DG TF Y + + E+ PL+ + + C T Y
Sbjct: 123 TVDG------------------TFQHYCIANATHATIIPESVPLEVAAPIMCAGITCY-R 163
Query: 633 ALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAK 785
AL +KV PG I R++ +D DK E+ K
Sbjct: 164 ALKESKVGPGEWICIPGAGGGLGHLAVQYAKAMAMRVVAIDTGDDKAELVK 214
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 47.6 bits (108), Expect = 2e-06
Identities = 51/232 (21%), Positives = 89/232 (38%), Gaps = 6/232 (2%)
Frame = +3
Query: 111 LAAVAWEAGKPLSIEEIEVDPPKAG---EVRVKITATGVCH-TDAYTLSGKDPEGVFPVV 278
+ A W+ PL+++ EV P +V VK TA +C +D++ SG+ P +
Sbjct: 37 MKACVWDG--PLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94
Query: 279 LXXXXXXXXXXXXXXXTSVKPGDHVVPLYVPQCNTCKFCLNPKTNLCQKVRSTQGQGVMP 458
L +++ GD VV + C C FC + C ++ V
Sbjct: 95 LGHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDV-N 153
Query: 459 DGTRRFRCKG--QELYHFMGCSTFSQYTVVLEISLCKVAEAAPLDKVSLLGCGVPTGYGA 632
G+ G + L GC EI+ CK+ + P D L V
Sbjct: 154 YGSHHSAIFGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIP-DSEGLFMSDVLCTSLH 212
Query: 633 ALNTAKVEPGSNCAIFXXXXXXXXXXXXXXXXXXNRIIGVDINPDKFEVAKK 788
A +V+ G AI+ +++IG+++ P++ E+A++
Sbjct: 213 ACTLGEVKKGDTVAIWGMGPIGLYAGRWAQILGASKVIGIEVVPERIELARQ 264
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 38.7 bits (86), Expect = 0.001
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 3/81 (3%)
Frame = +3
Query: 186 EVRVKITATGVCHTDA-YTLSGKDPEGVF--PVVLXXXXXXXXXXXXXXXTSVKPGDHVV 356
+V+V I ATG+C +D Y G + + P++L +S+KPGD V
Sbjct: 31 QVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGDPVA 90
Query: 357 PLYVPQCNTCKFCLNPKTNLC 419
C C +C + + NLC
Sbjct: 91 VEPGCVCRLCDYCRSGRYNLC 111
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 31.9 bits (69), Expect = 0.13
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +3
Query: 144 LSIEEIEVDPPKAGEVRVKITATGVCHTDAYTLSGKDPEGVFPVVL 281
+ I+ + + PK GE+ VKI A + +D +G P V+P ++
Sbjct: 23 IEIQSVPIPQPKNGELLVKIEAAAINPSDLMNATGGFPYTVYPRIV 68
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/47 (29%), Positives = 25/47 (53%)
Frame = -2
Query: 672 RNLILVQLWQYSRRLHNL*VHRIPAKKLYLMEQPLQLYREKFLEQLC 532
R+L V + R H L +H++ KK + +Q ++ R+ L+ LC
Sbjct: 210 RDLDAVACHERIARYHILCIHQLCEKKQFSAQQEVEQLRKGILQSLC 256
>SPBC29A10.07 |||nucleoporin Pom152|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1250
Score = 27.1 bits (57), Expect = 3.6
Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = +3
Query: 342 GDHVVPLY-VPQCNTCKFCLNPKTNLCQKVRSTQGQGVMPDGTRRFRCKGQEL 497
G HVV L V N C + KT KV + + P G+R++ C G L
Sbjct: 1036 GKHVVRLLQVRDANGCAASIT-KTQPAAKVSVVEMASLAPLGSRQYYCVGDRL 1087
>SPCC1020.05 |||phosphoprotein phosphatase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 509
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2
Query: 660 LVQLWQYSRRLHNL*VHRIPAKKLYLMEQPLQLY 559
+++ W+++R NL RIP K+ YL + LY
Sbjct: 70 VLEFWKWARTSRNLYRSRIPWKRAYLYVKRPALY 103
>SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 316
Score = 25.8 bits (54), Expect = 8.3
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +3
Query: 735 NRIIGVDINPDKFEVAKKLESMNLSTLR 818
NR G +FE A K E+ NLS+LR
Sbjct: 172 NRQKGTMTQQQRFEEAAKTEAQNLSSLR 199
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,353,178
Number of Sequences: 5004
Number of extensions: 64688
Number of successful extensions: 166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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