Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_D16
         (903 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa...    29   1.2  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    28   1.6  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    28   2.1  
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ...    27   3.6  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    27   3.6  
SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb...    26   6.4  
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb...    26   6.4  
SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ...    26   8.4  
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...    26   8.4  
SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase |Schizos...    26   8.4  
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe...    26   8.4  

>SPAC637.08 |||iron-sulfur cluster assembly ATPase
           Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 317

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 24/103 (23%), Positives = 41/103 (39%), Gaps = 1/103 (0%)
 Frame = +3

Query: 177 CPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTVEYHDVKTRGFDT-I 353
           CPN  +  I           + RGP  + L   + + D+   NL     D      D  +
Sbjct: 129 CPNLAVMSIGFLLPSEDSSVIWRGPKKNGLIKQFIK-DVNWENLDYLIVDTPPGTSDEHL 187

Query: 354 KIIEFYINSKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIV 482
            +++F+ NS  +  V+    Q + L   +    + RKA  PI+
Sbjct: 188 SLVQFFKNSGIDGAVVVTTPQEVALQDVRKEIDFCRKASIPIL 230


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
 Frame = +3

Query: 165 IKRPCPN-FDLNCIREYFSRNSQCQLVRGPVPDP 263
           I+ PC + F  NCI+ +   N  C + R PV DP
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGTCAICRAPV-DP 442


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 16/47 (34%), Positives = 28/47 (59%)
 Frame = +3

Query: 570  EVFSYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFE 710
            + + ++H IN  F+L  LLS SL +   SQ  KLL  +++  +++ E
Sbjct: 878  KTYGHLHYIN--FVLEKLLSSSLLTYNSSQRDKLLYEVSLLFKDLQE 922


>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
           Cho2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 905

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = -1

Query: 519 LEFHSQLPMRHFLQLALWLSYC 454
           LEF+S L  RHF+ L L   +C
Sbjct: 199 LEFNSWLVFRHFVDLILMCDFC 220


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
           domain|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1811

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
 Frame = +3

Query: 357 IIEFYIN-----SKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLT 521
           +IE Y+N       TE +   A V   ++AS  T    +      + R D L +D     
Sbjct: 527 LIELYLNYDCISGNTENIYERAIVTLSRIASQST----SDPPPSFVFRDDQLVIDKPGF- 581

Query: 522 FTAVFPSISDL-QLSNAEVFSYVHEINPKF 608
              V+ +++D+ QL+++ + SYVH  NP +
Sbjct: 582 ---VYHTLNDIPQLNSSTIGSYVHSHNPPY 608


>SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 952

 Score = 26.2 bits (55), Expect = 6.4
 Identities = 26/114 (22%), Positives = 54/114 (47%), Gaps = 1/114 (0%)
 Frame = +3

Query: 402 AAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLTFTAVF-PSISDLQLSNAEVF 578
           A E + L+++   T F  +RK  + I   + + ++   L    +   ++ ++  +  + F
Sbjct: 76  AREEKDLQVSRRDTCFYCSRKIIKCICNEEHVGIESVQLKLILLLCQNLPNVTTNAKKYF 135

Query: 579 SYVHEINPKFILGPLLSFSLDSETQSQLGKLLNNIAVSLQEVFEAQGTILMTSY 740
           S + +    F L  L  FSLD++T SQL     + A +L E+ +    +L  ++
Sbjct: 136 SSLAD-GHNFTL-TLYKFSLDNQTFSQLLSRFKSFA-TLTELLQVHNVMLQVNF 186


>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 741

 Score = 26.2 bits (55), Expect = 6.4
 Identities = 11/31 (35%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
 Frame = +3

Query: 168 KRPCPN-FDLNCIREYFSRNSQCQLVRGPVP 257
           K PC + F  NC++++   +  C L R  VP
Sbjct: 124 KMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154


>SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 678

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +3

Query: 561 SNAEVFSYVHEINPKFILGPLLSFSLDS 644
           +N  + S V  +NP  ++ PLL  SLD+
Sbjct: 44  TNRIMLSVVSSLNPDSLIAPLLCVSLDN 71


>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 20/82 (24%), Positives = 34/82 (41%)
 Frame = +3

Query: 135 TWIRPMTQKNIKRPCPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTV 314
           T I+P      K     FDLN    + S+   C L++   P+PL +        + N  +
Sbjct: 428 TNIQPELNDAEKSQLDGFDLNKTNWFDSKGLVCYLMKQTSPEPLLIRNLLFSFWSCNGKI 487

Query: 315 EYHDVKTRGFDTIKIIEFYINS 380
           E  D K +    + II + + +
Sbjct: 488 E-QDGKIKTATLVFIINYLLRT 508


>SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
 Frame = -1

Query: 306 NYYSVYLLCSNLKVVDLVQVPLLIDIENFLRNIHE----CSSSRSSDRV 172
           NY   YL    + V  L  +PLL  + N+ + ++E    CS+   ++R+
Sbjct: 316 NYRQSYLRIDAIAVHKLKSIPLLSTVSNWFKKMYEHRANCSALIETERL 364


>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 734

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 15/53 (28%), Positives = 24/53 (45%)
 Frame = -1

Query: 327 HHDILRLNYYSVYLLCSNLKVVDLVQVPLLIDIENFLRNIHECSSSRSSDRVF 169
           H D   +   S+ L    L  V  +Q+P L  +     N+H   SS+S+D  +
Sbjct: 136 HPDYPTIEELSLSLFREKLMAVKNIQIPFLNSLLQSFENLH---SSKSTDHAY 185


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,096,552
Number of Sequences: 5004
Number of extensions: 60165
Number of successful extensions: 162
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)