Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_D15
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 104 1e-23
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 100 2e-22
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 79 8e-16
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 73 4e-14
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 65 1e-11
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 31 0.22
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 28 2.0
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy... 27 3.6
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.2
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.2
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 26 8.2
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 26 8.2
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 104 bits (250), Expect = 1e-23
Identities = 50/111 (45%), Positives = 64/111 (57%)
Frame = +3
Query: 321 RTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGKAD 500
R ++QRG YLA +ETLD GK + + +V +A RYY G AD
Sbjct: 80 RKFSSAQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGGWAD 139
Query: 501 KILGNTIPSDGEVLTFTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAGCT 653
K G TI +D + +T EP+GVCGQIIPWN+P M +WKIAPA+A G T
Sbjct: 140 KDYGQTIETDIKRFAYTRHEPIGVCGQIIPWNFPFLMCAWKIAPAVACGNT 190
Score = 57.2 bits (132), Expect = 3e-09
Identities = 25/52 (48%), Positives = 37/52 (71%)
Frame = +2
Query: 728 FHRGVVNVVPGYGATAGGALTHXPDVDKIAFTGSTEVGRIILXAXSAVNLKR 883
F GV+NV+ G G G A++ D+DK+AFTGST VGR+++ A ++ NLK+
Sbjct: 215 FPPGVINVLSGDGRRCGNAISSHMDIDKVAFTGSTGVGRMVMRAAASSNLKK 266
Score = 47.6 bits (108), Expect = 2e-06
Identities = 28/80 (35%), Positives = 42/80 (52%), Gaps = 2/80 (2%)
Frame = +1
Query: 130 KVDVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAFHRL 309
K ++ LFINN+ VD+V +P E +I +VA+ D+ D+D+AV A+ AF
Sbjct: 17 KTYIQPVGLFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQ-- 74
Query: 310 FPMAVPWTLLNAA--SYCLS 363
PW ++A CLS
Sbjct: 75 --TDAPWRKFSSAQRGRCLS 92
Score = 30.7 bits (66), Expect = 0.29
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +1
Query: 655 VVLKPAEQTPLTXXXXXXXXKEAGFPPG 738
++LK AE TPL+ E GFPPG
Sbjct: 191 IILKTAELTPLSALCLTKFVPECGFPPG 218
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 100 bits (240), Expect = 2e-22
Identities = 48/110 (43%), Positives = 66/110 (60%), Gaps = 1/110 (0%)
Frame = +3
Query: 321 RTMDASQRGXXXXXXXXXXXSQSRYLAELETLDCGKP-VKQSEEEVYFSASVLRYYAGKA 497
+ + S++G + LA +E +D GKP V + +V + ++LRY AG A
Sbjct: 73 KKVPGSEKGELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWA 132
Query: 498 DKILGNTIPSDGEVLTFTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAG 647
DKI G IP+ E L + + P+GVCGQI+PWNYP+ M WKIAPALAAG
Sbjct: 133 DKIYGQVIPTGPEKLAYAKRTPIGVCGQIVPWNYPLNMAGWKIAPALAAG 182
Score = 55.2 bits (127), Expect = 1e-08
Identities = 26/51 (50%), Positives = 35/51 (68%)
Frame = +2
Query: 728 FHRGVVNVVPGYGATAGGALTHXPDVDKIAFTGSTEVGRIILXAXSAVNLK 880
F +GVVN++ G G AG + P +DKIAFTGST+VG +I+ +A NLK
Sbjct: 209 FPKGVVNIISGLGTVAGSYMAKHPGIDKIAFTGSTKVG-VIVQQLAASNLK 258
Score = 29.5 bits (63), Expect = 0.67
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 655 VVLKPAEQTPLTXXXXXXXXKEAGFPPG 738
+++K AE TPL+ +EAGFP G
Sbjct: 185 IIIKSAETTPLSLLYFATLVEEAGFPKG 212
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 79.0 bits (186), Expect = 8e-16
Identities = 44/140 (31%), Positives = 77/140 (55%), Gaps = 1/140 (0%)
Frame = +3
Query: 396 LAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADKILGNT-IPSDGEVLTFTMKEPVGV 572
LA + TL+ GK + ++ EV ++A + ++AG+A +I G++ + S+ + T+K+PVGV
Sbjct: 103 LATMMTLENGKALGDAKGEVVYAAKFIDWFAGEALRISGDSSMSSNPQNRIITIKQPVGV 162
Query: 573 CGQIIPWNYPIPMMSWKIAPALAAGCTGCSEAS*TNTTNGARCCRPGQRSGFSTGGLLMS 752
G I PWN+P M++ K+ ALAAGCT + + +R+G G L M
Sbjct: 163 VGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAGVPAGVLNMV 222
Query: 753 FPDTVRQRAVLLLTXQMLTK 812
++ + + L T ++ K
Sbjct: 223 TANSPSEHGIELTTNPLIRK 242
Score = 34.3 bits (75), Expect = 0.023
Identities = 21/50 (42%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +2
Query: 737 GVVNVVPGYGATAGGA-LTHXPDVDKIAFTGSTEVGRIILXAXSAVNLKR 883
GV+N+V + G LT P + K++FTGST VG+ IL S+ LK+
Sbjct: 217 GVLNMVTANSPSEHGIELTTNPLIRKVSFTGSTNVGK-ILAKQSSSTLKK 265
Score = 29.9 bits (64), Expect = 0.50
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +1
Query: 157 FINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAF 300
+I +WV A S KTF NP + V + + A+ A +AF
Sbjct: 27 YIGGKWVTAASGKTFDVENPGLNETLAPVTDMSVEETRKAIKVAHEAF 74
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 73.3 bits (172), Expect = 4e-14
Identities = 37/88 (42%), Positives = 55/88 (62%), Gaps = 2/88 (2%)
Frame = +3
Query: 396 LAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADKILGNTIPSDGEVLTF--TMKEPVG 569
L ++ TL+ GKP+ Q+E EV + L++YA +A + G+ PS + F ++K+PVG
Sbjct: 97 LVKMLTLENGKPLSQAEMEVTTCSGYLKWYAAEAVRTFGDVAPSSLQSQNFLISIKQPVG 156
Query: 570 VCGQIIPWNYPIPMMSWKIAPALAAGCT 653
V I PWN+P M++ K ALAAGCT
Sbjct: 157 VSALITPWNFPAAMIARKGGAALAAGCT 184
Score = 41.5 bits (93), Expect = 2e-04
Identities = 24/53 (45%), Positives = 34/53 (64%), Gaps = 1/53 (1%)
Frame = +2
Query: 728 FHRGVVNVVPGYGATAGGA-LTHXPDVDKIAFTGSTEVGRIILXAXSAVNLKR 883
F GV+NV+ A+A G LT P V K++FTGST VG+I++ SA +K+
Sbjct: 209 FPDGVLNVITSSDASAHGKELTTNPIVRKVSFTGSTNVGKILM-GQSASTIKK 260
Score = 37.9 bits (84), Expect = 0.002
Identities = 16/55 (29%), Positives = 30/55 (54%)
Frame = +1
Query: 136 DVKYTKLFINNEWVDAVSKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAF 300
D + + F+ +W+ + + KTF NP +I +VA+ + A++AA +AF
Sbjct: 14 DKSHAQSFVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAF 68
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 64.9 bits (151), Expect = 1e-11
Identities = 33/106 (31%), Positives = 53/106 (50%), Gaps = 3/106 (2%)
Frame = +3
Query: 339 QRGXXXXXXXXXXXSQSRYLAELETLDCGKPVKQSEEEVYFSASVLRYYAGKADK---IL 509
QRG + LA ++T++CGKP + ++ A + YYA A+ +
Sbjct: 85 QRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALFDIDSCADMFEYYAEVAETDNPTV 144
Query: 510 GNTIPSDGEVLTFTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAG 647
+P++ F + P GV G I PWN+P+ M WK+ PA+A+G
Sbjct: 145 KVPLPNNPGFCAFEKRFPRGVIGVITPWNFPLKMALWKLVPAIASG 190
Score = 38.3 bits (85), Expect = 0.001
Identities = 18/41 (43%), Positives = 26/41 (63%)
Frame = +2
Query: 737 GVVNVVPGYGATAGGALTHXPDVDKIAFTGSTEVGRIILXA 859
GV+NV+ G G +G AL+ P + +AFTGS G+ I+ A
Sbjct: 220 GVLNVIIGSGKESGAALSCHPKIAYLAFTGSLATGKKIMHA 260
Score = 32.3 bits (70), Expect = 0.094
Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = +1
Query: 154 LFINNEWVDAV--SKKTFPTINPQDETVITQVAEGDKADIDLAVAAAKKAF 300
LFI+ ++V + + K P INP E +I A D+D AV A F
Sbjct: 23 LFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTF 73
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +1
Query: 655 VVLKPAEQTPLTXXXXXXXXKEAGFPPG 738
VVLKP+E P + KEAG P G
Sbjct: 193 VVLKPSELAPWSCLEFALICKEAGLPDG 220
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 31.1 bits (67), Expect = 0.22
Identities = 26/95 (27%), Positives = 42/95 (44%), Gaps = 6/95 (6%)
Frame = +3
Query: 381 SQSRYLAELETLDCGKP-VKQSEEEVYFSASVLRYYAGKADKILGNTIPSDGEVLT---- 545
+Q +Y AE+ D GK V + E+ + + + ++ L T +LT
Sbjct: 63 NQDKY-AEIACKDTGKTLVDAAFGEILVTLEKINWTLANGEQSLRPT-KRPNSLLTSYKG 120
Query: 546 -FTMKEPVGVCGQIIPWNYPIPMMSWKIAPALAAG 647
+ EP+GV ++ WNYP+ I AL AG
Sbjct: 121 GYVKYEPLGVIAALVSWNYPLHNALGPIISALFAG 155
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 27.9 bits (59), Expect = 2.0
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +1
Query: 157 FINNEWVDAVSKKTFPTINPQDETVITQVAEGDKAD 264
F+ D S+ TF TI PQ+ + E K+D
Sbjct: 224 FLQQSEADQPSEDTFTTIGPQNSLTVNSTIEKHKSD 259
>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 27.1 bits (57), Expect = 3.6
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = -1
Query: 284 AATAKSISALSPSATCVMTVSSCGLMVGKVFLLTASTHSLLINSLVYLTST 132
+ +A +SA S + TC +S+ G LTA ++++ NS ST
Sbjct: 442 SGSATLVSASSATGTCASYLSAAGSSATNAISLTADSNAVSRNSSASTMST 492
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.2
Identities = 22/73 (30%), Positives = 33/73 (45%)
Frame = -2
Query: 571 TPTGSFMVNVRTSPSEGMVFPNILSAFPA*YLSTLAEKYTSSSDCFTGFPQSKVSSSARY 392
+PT S + +S S F + LS+ S+ + TSSS + S SSS
Sbjct: 299 SPT-STSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSS 357
Query: 391 LDWDSRSSASLSN 353
S+SS+S S+
Sbjct: 358 TTSSSKSSSSFSS 370
>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 452
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = -1
Query: 329 HGTAMGNXRWNAFFAAATAKSISALSPSATCVMTVSS 219
+ T + WN+ +AAT S S+ S S + V++V+S
Sbjct: 155 NSTTNSSISWNSSTSAATNTSSSSSSSSQSSVVSVNS 191
>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1052
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2
Query: 569 SVRSDNTVELPHTNDVLENSTSLSCRMYR 655
SVR +NT +L D++E + R++R
Sbjct: 357 SVRDENTKKLKELQDIIEEQSKHMARLHR 385
>SPAC1093.03 |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = +2
Query: 437 TIRRRSILF-RKRAQIL--RWKSGQDIRKYHSFRRRSPYVHHEGTCRSVRSDN 586
T+ + ++F KR Q L ++K+GQ +R+ + + YV+ E C++ D+
Sbjct: 543 TVHGKIVVFGEKRLQALTEKFKNGQLVRRDYRYWWTPVYVNQELRCKNAYCDS 595
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,495,568
Number of Sequences: 5004
Number of extensions: 73370
Number of successful extensions: 209
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 182
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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