Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_D06
(953 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 29 1.3
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 29 1.3
SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ... 28 1.7
SPBC428.01c |nup107|SPBC582.11c|nucleoporin Nup107|Schizosacchar... 27 3.9
SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces pom... 27 3.9
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar... 27 5.1
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz... 26 6.8
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 26 6.8
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 26 6.8
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac... 26 6.8
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 6.8
SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi... 26 6.8
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 28.7 bits (61), Expect = 1.3
Identities = 13/47 (27%), Positives = 28/47 (59%)
Frame = +3
Query: 291 GQVSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEF 431
G ++K E+ ++++F P + + +++F + DA +G ID +EF
Sbjct: 41 GHLNKSEFQKIYKQFF--PFGDPSAFAEYVFNVFDADKNGYIDFKEF 85
Score = 27.5 bits (58), Expect = 2.9
Identities = 15/66 (22%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
Frame = +3
Query: 402 NDGSIDSEEFSSVYASFGLDKAEAASAFQKLSKGKSS--VSFAEFQELFKEYFASEDVNA 575
+ + ++ + S DK E ++ K S ++ +EFQ+++K++F D +A
Sbjct: 4 SQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSA 63
Query: 576 PGNFVF 593
+VF
Sbjct: 64 FAEYVF 69
Score = 26.6 bits (56), Expect = 5.1
Identities = 33/118 (27%), Positives = 51/118 (43%), Gaps = 9/118 (7%)
Frame = +3
Query: 105 HVFTAFFDTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTD 284
+VF F D + +G ID K+F A+ SRG E+ D L +W D D
Sbjct: 67 YVFNVF-DADKNGYIDFKEFICALS--VTSRG---------ELNDKL--IW-AFQLYDLD 111
Query: 285 NDGQVSKEEWI----SLWEKFSS-----SPSDWQNLYCKFIFQLEDASNDGSIDSEEF 431
N+G +S +E + ++++ S D IF + D + DG + EEF
Sbjct: 112 NNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLEEF 169
>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 836
Score = 28.7 bits (61), Expect = 1.3
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -1
Query: 140 AAIGVEESGENVQQLLLSKI*HHFADLSRLII 45
+A+G+EE + L K HHFADL + +
Sbjct: 85 SAVGIEEKSFKDEHLAQKKGLHHFADLKEIFL 116
>SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 28.3 bits (60), Expect = 1.7
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +3
Query: 279 TDNDGQVSKEEWISLWE---KFSSSPSDWQNLY 368
T+ DG EE+ ++ KF++ P+ WQN Y
Sbjct: 240 TEEDGDEKDEEYSDIFSIKGKFNTRPNAWQNAY 272
>SPBC428.01c |nup107|SPBC582.11c|nucleoporin
Nup107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 794
Score = 27.1 bits (57), Expect = 3.9
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +3
Query: 306 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSS 437
EEWI LW+ S + W + + F+L + G I S FSS
Sbjct: 100 EEWIELWDLESRT---WDLIQRLYSFRLSE--QQGHIQSHAFSS 138
>SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 27.1 bits (57), Expect = 3.9
Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +3
Query: 267 SSADTDNDGQVSKEEWISLWEKFS-SSPS 350
SS DTD+DGQ+ EE K S SSPS
Sbjct: 245 SSEDTDSDGQMEDEEKSDSVHKSSFSSPS 273
>SPBC530.14c |dsk1||SR protein-specific kinase
Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 544
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +1
Query: 286 TTAKSPKKSGFPSGRSFPAAPVTGRI 363
T AK+P K+ P+G+ P++P T +
Sbjct: 278 TFAKNPTKNSKPAGQVIPSSPFTSTL 303
>SPBC354.05c |sre2||membrane-tethered transcription factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 793
Score = 26.2 bits (55), Expect = 6.8
Identities = 20/58 (34%), Positives = 24/58 (41%), Gaps = 3/58 (5%)
Frame = +1
Query: 280 PITTAKSPKKSGFPSGRSFPAA---PVTGRIYTASLYFNLRMLVMTGPSTARNSLRST 444
PI+ S G P S +A V AS FNL V P ARNS+ S+
Sbjct: 57 PISHVPSVGTRGIPRSYSMASAYPYQVNSNSMDASTAFNLNQDVADKPMLARNSIDSS 114
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 26.2 bits (55), Expect = 6.8
Identities = 31/108 (28%), Positives = 44/108 (40%)
Frame = +3
Query: 126 DTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTDNDGQVSK 305
D +G+GTID +F + R K D +E KV+D D +G ++
Sbjct: 58 DADGNGTIDFTEFLTMMARKMK-------DTDNEEEVREAFKVFD------KDGNGYITV 104
Query: 306 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSSVYAS 449
EE + S Q I + D DG I+ EEFS V +S
Sbjct: 105 EELTHVLTSLGERLS--QEEVADMIREA-DTDGDGVINYEEFSRVISS 149
>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1210
Score = 26.2 bits (55), Expect = 6.8
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 106 TFSPLSSTPMAAEPLIKRTLNLPLKGYPNREAGVL 210
TF+ SS P + P +R +++ ++ PN + G++
Sbjct: 876 TFTVESSQPFSLSPGERREIHIKIRAKPNSQEGLI 910
>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
Hip3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1630
Score = 26.2 bits (55), Expect = 6.8
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -2
Query: 205 LQPLDLDILSMASSKSFLSMVPLPLVSKKAVKTC 104
L LDL I S+ + SF +P P ++++ C
Sbjct: 201 LPQLDLSISSLYTLPSFFPQLPTPSFHRRSINLC 234
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 26.2 bits (55), Expect = 6.8
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +3
Query: 282 DNDGQVSKEEWISLWEKFSSSPSDWQN 362
DND Q + W S+ F+ P ++ N
Sbjct: 253 DNDSQTRRSSWSSIASAFNDFPEEFPN 279
>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
Tom70|Schizosaccharomyces pombe|chr 1|||Manual
Length = 625
Score = 26.2 bits (55), Expect = 6.8
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +3
Query: 465 AEAASAFQKLSKGKSSVSFAEFQELFKEYFASEDVNAPGNFVFGKTSF 608
A A A++ L KG+ +S+ + +E F+S VNA + + G F
Sbjct: 311 AALAEAYEYLEKGEYQLSYDKAKESCLGSFSSPSVNARTHNLVGTFKF 358
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,309,578
Number of Sequences: 5004
Number of extensions: 68910
Number of successful extensions: 251
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 487313384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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