Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_C19
         (890 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    31   0.22 
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb...    28   1.6  
SPAP19A11.05c |mrp7||mitochondrial ribosomal protein subunit L27...    27   2.7  
SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|ch...    27   4.7  
SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces po...    26   6.3  
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy...    26   8.3  

>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 3227

 Score = 31.1 bits (67), Expect = 0.22
 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
 Frame = +2

Query: 527  STMLRWSLTKFPKTYTIGE-AMIVTQGFDFIHHCSYWKDH 643
            ST L  SL K P    I   A+I+ QG D + H + W DH
Sbjct: 2456 STGLLESLCKVPVVNGISAPALILQQGIDLLSHLATWADH 2495


>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 542

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 22/90 (24%), Positives = 39/90 (43%)
 Frame = +2

Query: 278 LISYNFLYKVSPLYTLVAYVAVGLFCYSILFVVFISVSCLVLREPIYGGCIASSLVSALM 457
           L+S   LY      +LV    +  F   +LF+ F+  SC+V  E  +      + +  L+
Sbjct: 361 LVSERMLYLSLMYKSLVDLKTIDSFSLKLLFLKFMICSCMVKGESNF---FLDNRI-FLL 416

Query: 458 NFIFNTQGLTTSLSFSLTAVYLYSTMLRWS 547
             I N  G+  ++   L   ++ +   RWS
Sbjct: 417 ERIMNRYGIPMTIDTFLLMQFILAKSNRWS 446


>SPAP19A11.05c |mrp7||mitochondrial ribosomal protein subunit
           L27|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 153

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 7/31 (22%), Positives = 19/31 (61%)
 Frame = +2

Query: 683 KQHCAYSIINGWITYYRXXCDIGKTKKHTNI 775
           K H  YS+++G++ +++    + K +K+  +
Sbjct: 90  KDHTIYSLVSGYVKFFQMDVSLRKRRKYVGV 120


>SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 546

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 12/48 (25%), Positives = 25/48 (52%)
 Frame = +2

Query: 257 CALLPALLISYNFLYKVSPLYTLVAYVAVGLFCYSILFVVFISVSCLV 400
           C LLP  +  + +      ++ +V  +A   F +S+L V F+S + ++
Sbjct: 423 CFLLPISMFWFAWTCYPHHIHWIVPIIASAFFGFSLLIVFFVSYNYII 470


>SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 166

 Score = 26.2 bits (55), Expect = 6.3
 Identities = 22/101 (21%), Positives = 41/101 (40%)
 Frame = +2

Query: 275 LLISYNFLYKVSPLYTLVAYVAVGLFCYSILFVVFISVSCLVLREPIYGGCIASSLVSAL 454
           L +S +F+      Y L A++A+  F  +   V     S ++   P      +SS     
Sbjct: 67  LSLSVSFITHGDNAYLLPAWIAISCFQTAAYIVQDWITSPIIRTLPFRSSSSSSSNYRHN 126

Query: 455 MNFIFNTQGLTTSLSFSLTAVYLYSTMLRWSLTKFPKTYTI 577
           ++F+  T      +     A +    ML W L K+P ++ +
Sbjct: 127 LDFLEITVFAVVPVGI---ASFFTMVMLIWQLYKYPDSFLV 164


>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1010

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 11/33 (33%), Positives = 15/33 (45%)
 Frame = -1

Query: 377 IPQTVCCSRINQQLRKLLVYKVATLCTKNCMRL 279
           IP+  C   I  +L  +L Y +  LC   C  L
Sbjct: 806 IPKAFCAVEIVDRLAAMLNYNLQALCGPKCSNL 838


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,443,590
Number of Sequences: 5004
Number of extensions: 72030
Number of successful extensions: 173
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 448490560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)