Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP20_F_C18
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyce... 270 2e-73
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 73 7e-14
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 69 9e-13
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 52 1e-07
SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyce... 52 1e-07
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 44 2e-05
SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomy... 38 0.002
SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 29 0.87
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 29 1.1
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 27 4.6
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 27 4.6
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce... 26 6.1
SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane synthase|Schiz... 26 6.1
SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 26 8.1
>SPCC63.12c |||20S proteasome component beta 3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 204
Score = 270 bits (661), Expect = 2e-73
Identities = 119/205 (58%), Positives = 162/205 (79%)
Frame = +2
Query: 152 MSILAYNGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDT 331
MSI+ YNGG+ VAM G++CVAIA+D R G+Q+ +++ NFPKVF MG Y+GL GLATD
Sbjct: 1 MSIMEYNGGSCVAMAGKNCVAIASDLRLGVQSISLTNNFPKVFAMGDKTYLGLTGLATDV 60
Query: 332 QTVFQRLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPY 511
QT+++ ++++NLY+ +E R ++PKTF+ ++S+ LYE+RFGPYF PV+AG+ DN P+
Sbjct: 61 QTLYELFRYKVNLYKFREERQIQPKTFANLVSSTLYEKRFGPYFSFPVVAGVS-NDNTPF 119
Query: 512 VCNMDLIGCPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAI 691
+C D IGC + EDF+VSGT +EQLYGMCE+++EPNL+PD+LFETISQAL+NA DRD I
Sbjct: 120 ICGFDSIGCIDFAEDFIVSGTATEQLYGMCESVYEPNLEPDDLFETISQALLNAQDRDCI 179
Query: 692 SGWGAVVYIIEKDKITEKHVKTRMD 766
SGWG VVY+I DK ++ VK R D
Sbjct: 180 SGWGCVVYVITADKCIKRLVKGRQD 204
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 72.5 bits (170), Expect = 7e-14
Identities = 37/123 (30%), Positives = 66/123 (53%), Gaps = 1/123 (0%)
Frame = +2
Query: 170 NGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNF-PKVFQMGPTLYVGLPGLATDTQTVFQ 346
NGG VA+ G +A D R + ++T F P+V ++G L +G G D + +
Sbjct: 12 NGGTTVAIAGDGFAILAGDTR-SVNGYNINTRFQPRVHEVGDDLVIGASGFEADALALVK 70
Query: 347 RLKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMD 526
R++ R++LY R M ++ + M+ LLY +RF PY++ +AG+D + + + + D
Sbjct: 71 RIQQRIDLYHDNHERKMSAQSCACMVRTLLYGKRFFPYYVYTTVAGIDK-EGKGEIYSFD 129
Query: 527 LIG 535
+G
Sbjct: 130 PVG 132
>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 68.9 bits (161), Expect = 9e-13
Identities = 48/191 (25%), Positives = 90/191 (47%)
Frame = +2
Query: 173 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 352
G + A++ +D V +A D R + A + K+ Q+ ++ G A DTQTV L
Sbjct: 24 GTTITALRYKDGVILAADSRTTMGAYIANRVTDKLTQLTDNIWCCRSGSAADTQTVADLL 83
Query: 353 KFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDLI 532
K+ +++Y ++ T + + S + Y+ + ++AG D V ++ L
Sbjct: 84 KYYLSMYRIQFGHDPSVHTAATLASEMCYQNK-NMLSAGLIVAGYDEKTGGD-VYSIPLG 141
Query: 533 GCPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGWGAVV 712
G ++ + + G+ S +YG C+A + N+ +E E + A+ A +RD SG +
Sbjct: 142 GSLHK-QPLAIGGSGSAFIYGFCDANFRENMTQEEAVEFLKNAVALAMERDGSSGGTIRM 200
Query: 713 YIIEKDKITEK 745
I+ KD + K
Sbjct: 201 VILNKDGMERK 211
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 52.0 bits (119), Expect = 1e-07
Identities = 45/187 (24%), Positives = 79/187 (42%)
Frame = +2
Query: 170 NGGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQR 349
+G +A + Q + + D R S KV ++ P L L G A D Q
Sbjct: 60 HGTTTLAFRYQHGIVVCVDSRASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETV 119
Query: 350 LKFRMNLYELKENRMMRPKTFSAMLSNLLYERRFGPYFIEPVIAGLDPYDNQPYVCNMDL 529
L L++L+ ++ S +LSN+ Y + + ++AG Y + D
Sbjct: 120 LGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMGTMLAGTGKGGTALYYIDSD- 178
Query: 530 IGCPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGWGAV 709
G + + F V G+ S YG+ ++ + +L E +++V A RDA SG
Sbjct: 179 -GTRLKGDLFSV-GSGSTFAYGVLDSGYRWDLSKQEALYLAQRSIVAATHRDAYSGGSVN 236
Query: 710 VYIIEKD 730
+Y I+++
Sbjct: 237 LYHIDEN 243
>SPBC577.10 |||20S proteasome component beta 7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 262
Score = 52.0 bits (119), Expect = 1e-07
Identities = 37/125 (29%), Positives = 62/125 (49%), Gaps = 4/125 (3%)
Frame = +2
Query: 173 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 352
G +V+A+K D V IA D + + ++ ++G VG G +D Q + QRL
Sbjct: 43 GSSVLALKFADGVMIAADNLASYGSLARFYDVERLTKVGDNTIVGAGGDISDYQQI-QRL 101
Query: 353 KFRMNLYE--LKENRMMRPKTFSAMLSNLLYERR--FGPYFIEPVIAGLDPYDNQPYVCN 520
++ + E + ++P LS +LY RR PY+ + ++AG+D + +PYV
Sbjct: 102 LEKLEIKEGNYGDGYALQPSYIHEYLSKVLYARRNKLDPYWNQLIVAGVDGENKEPYVAF 161
Query: 521 MDLIG 535
DL G
Sbjct: 162 ADLRG 166
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 44.4 bits (100), Expect = 2e-05
Identities = 36/169 (21%), Positives = 72/169 (42%), Gaps = 4/169 (2%)
Frame = +2
Query: 173 GGAVVAMKGQDCVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLATDTQTVFQRL 352
G +A++G +C+ I +++ + Q VS NF K+ + + + GL D + + +
Sbjct: 30 GTTAIALRGNECIVIGVERKNVPKLQNVS-NFQKIAMVDNHVCLAFAGLNADARILIDKA 88
Query: 353 KFRMNLYELKENRMMRPKTFSAMLSNLL--YERRFG--PYFIEPVIAGLDPYDNQPYVCN 520
+ ++L + + + ++ + Y + G P+ + +IAG D DN P V
Sbjct: 89 RVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPRVYQ 148
Query: 521 MDLIGCPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALV 667
+ G N + + G S+ E W+ L DE +L+
Sbjct: 149 TEPAGIYNAWKATAI-GRASKAAREYLEKNWKEGLSRDETIHLAVSSLL 196
>SPAC31A2.04c |||20S proteasome component beta 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 194
Score = 37.5 bits (83), Expect = 0.002
Identities = 42/197 (21%), Positives = 81/197 (41%), Gaps = 6/197 (3%)
Frame = +2
Query: 179 AVVAMKGQDCVAIATDKRF--GIQAQTVSTNFPKVFQM-GPTLYVGLPGLATDTQTVFQR 349
+++A++GQD V A+ GI + ++ LY G G DT +
Sbjct: 3 SLLAVQGQDFVLTASSSSAVRGITVLKPDDDKSQILNSHNLMLYCGEAG---DTTNFAEY 59
Query: 350 LKFRMNLYELKENRMMRPKTFSAMLSNLLYE--RRFGPYFIEPVIAGLDPYDNQPYVCNM 523
+ ++LY L+ N + P+ ++ L R PY + ++AG + +P + +
Sbjct: 60 IAANISLYTLRHNLNLSPEATASFTRKQLATSLRSRKPYQVNILLAGYETNLGKPELFWL 119
Query: 524 DLIG-CPNEPEDFVVSGTCSEQLYGMCEALWEPNLKPDELFETISQALVNAADRDAISGW 700
D + C P + G S + + ++P+L DE + R I
Sbjct: 120 DYLATCVRVP--YACQGYSSFYCLSIFDRYYKPDLTIDEAVRIMKLCFDELKKRMPIDFK 177
Query: 701 GAVVYIIEKDKITEKHV 751
G + +++KD I E ++
Sbjct: 178 GFICKVVDKDGIREINI 194
>SPAC1F8.07c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 594
Score = 29.1 bits (62), Expect = 0.87
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 672 RQIVTPSPVGVPLFTSLKKTRSLRNTSRLGWINY 773
+ + P+PV +PL T LK+T +N L + NY
Sbjct: 555 KAVAPPTPVLLPLITRLKRTLFGKNMLHLDYKNY 588
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = +2
Query: 176 GAVVAMKGQD-CVAIATDKRFGIQAQTVSTNFPKVFQMGPTLYVGLPGLAT 325
GA+ +D VA A D G+ S + +VF++G L VG+ G T
Sbjct: 407 GALFKFDTEDEVVAWANDSPVGLAGYLFSKDISRVFRVGEALQVGMVGCNT 457
>SPAC328.04 |||AAA family ATPase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 26.6 bits (56), Expect = 4.6
Identities = 18/49 (36%), Positives = 26/49 (53%)
Frame = -1
Query: 702 PQPEMASRSAAFTRACEIVSKSSSGFKLGSQRASHIPYSCSEQVPDTTK 556
P P+ ++ A R +S SSS FK +Q SH+P +E VP +K
Sbjct: 267 PTPDNSAVIEAARRTYSSISSSSSPFKKKTQ--SHLPNRTTE-VPSISK 312
>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 981
Score = 26.6 bits (56), Expect = 4.6
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = +2
Query: 80 IQYFELITLQ*FQVKKCLSFYITNMSIL----AYNGGAVVAMK 196
++YF+ + + +VK SF+I N +L AYNG V +K
Sbjct: 441 LKYFDQVVVGLKEVKDAFSFFIFNRFVLVYVPAYNGVDPVTLK 483
>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 512
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = +1
Query: 202 RLCGHRYGQAVWYTSSNSINQLPKSIPNGTHIV 300
RL G Y V Y+S NS+ LPK P+ H+V
Sbjct: 239 RLRGKTYDYKVEYSSINSLFLLPK--PDEQHVV 269
>SPAC24B11.13 |hem3|SPAC806.01|hydroxymethylbilane
synthase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 336
Score = 26.2 bits (55), Expect = 6.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 250 NSINQLPKSIPNGTHIVCRPSRS 318
+S+ LP +P+G I C P RS
Sbjct: 84 HSLKDLPSEMPDGMVIACIPKRS 106
>SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 373
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2
Query: 632 DELFETISQALVNAADRDAISG 697
DEL +T+S +AAD DA+ G
Sbjct: 298 DELLDTLSTTSPDAADDDALQG 319
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,575,056
Number of Sequences: 5004
Number of extensions: 79457
Number of successful extensions: 219
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 203
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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