Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP20_F_B23
         (908 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ...   322   4e-89
SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit S5|S...    29   0.69 
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S...    29   0.91 
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    28   2.1  
SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces p...    26   8.5  

>SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score =  322 bits (791), Expect = 4e-89
 Identities = 141/215 (65%), Positives = 178/215 (82%)
 Frame = +3

Query: 219 KEDQKEWVPVTKLGRLVREGKIDKLESIYLFSLPIKEFEIIDFFLGPSLNDEVLKIMPVQ 398
           ++++KEWVPVTKLGRLV+ GKI  +E IYL+SLPIKE++I+D+FL P LNDEV+K++PVQ
Sbjct: 28  RDEEKEWVPVTKLGRLVKAGKIKSIEEIYLYSLPIKEYQIVDYFL-PRLNDEVMKVVPVQ 86

Query: 399 KQTRAGQRTRFKAFVAIGDNNGHIGLGVKCSKEVATAIRGAIILAKLSVLPVRRGYWGNK 578
           KQTRAGQRTRFKAFV IGD++GH+GLG+KC+KEVATAIRGAII+ KLS++P+RRGYWG  
Sbjct: 87  KQTRAGQRTRFKAFVVIGDSDGHVGLGIKCAKEVATAIRGAIIMGKLSIMPIRRGYWGTA 146

Query: 579 IGKPHTVPCKVTGKCGSVTVRLIPAPRGTGIVSAPVPKKLLQMAGVQDCYTSARGSTGTL 758
           +G PHTVP KV+GKCGSVTVRL+PAPRG G+V+APV K+ LQ+AG++DCYT +RGST TL
Sbjct: 147 LGDPHTVPVKVSGKCGSVTVRLVPAPRGAGLVAAPVTKRFLQLAGIEDCYTQSRGSTKTL 206

Query: 759 GNFXXXXXXXXXXXXXXLTPDLWXDXPLTKSPYSE 863
           GNF              LTP+LW + P  ++P  E
Sbjct: 207 GNFVKAAFAAASLTYGILTPNLWAERPFGQTPIEE 241


>SPAC4G9.17c |mrps5||mitochondrial ribosomal protein subunit
           S5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 387

 Score = 29.5 bits (63), Expect = 0.69
 Identities = 28/118 (23%), Positives = 50/118 (42%)
 Frame = +3

Query: 393 VQKQTRAGQRTRFKAFVAIGDNNGHIGLGVKCSKEVATAIRGAIILAKLSVLPVRRGYWG 572
           V  QTR G+         +G+ NG  G G   ++  + A + +   A  +++ + R Y  
Sbjct: 235 VVNQTRKGKIASMYVLTVVGNRNGVAGFGEGKAESYSLAYKQSCGRAVKNMVYIPR-Y-- 291

Query: 573 NKIGKPHTVPCKVTGKCGSVTVRLIPAPRGTGIVSAPVPKKLLQMAGVQDCYTSARGS 746
                  TV   +  K  +V + L   P G G+   P+  ++ + AG++D      GS
Sbjct: 292 ----DKRTVYGVIHKKFHAVRLTLRSRPAGFGLRCNPILHEICRCAGIKDISGEILGS 345


>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 971

 Score = 29.1 bits (62), Expect = 0.91
 Identities = 12/20 (60%), Positives = 16/20 (80%)
 Frame = +1

Query: 691 RSFFRWLVYRTATPQLVVQL 750
           RS F+WL+  TATP+L+V L
Sbjct: 69  RSVFQWLIALTATPRLLVLL 88


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 13/42 (30%), Positives = 21/42 (50%)
 Frame = -3

Query: 216 RAHDHGRDHDRVHEDRRGLYLHRVIRIRRENRHVHRLEQRPP 91
           ++HDHG  H + H DR      +  R  R++R     ++ PP
Sbjct: 720 QSHDHGHSHSKSH-DREKEKEKKKDREHRKHRETEEEDEGPP 760


>SPAC140.01 |sdh2||succinate dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 252

 Score = 25.8 bits (54), Expect = 8.5
 Identities = 10/29 (34%), Positives = 15/29 (51%)
 Frame = +2

Query: 584 KATHRPLQGHRQVWFCNSPADSCPSWYWN 670
           +A    L G  +   C   + SCPS++WN
Sbjct: 153 RADRAKLDGLYECILCACCSTSCPSYWWN 181


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,499,710
Number of Sequences: 5004
Number of extensions: 73745
Number of successful extensions: 223
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 460503700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)