BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_P16
(1007 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.34
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 0.54
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.59
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 0.78
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.4
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.8
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 26 7.3
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 22 8.2
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.34
Identities = 13/27 (48%), Positives = 14/27 (51%), Gaps = 4/27 (14%)
Frame = -1
Query: 248 PPPXXFLFXPPPPXPP----XXXPPPP 180
PPP + PPPP PP PPPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPP 778
Score = 27.9 bits (59), Expect = 2.4
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = -1
Query: 251 PPPPXXFLFXPPPPXPPXXXPPP 183
PPPP + P P P PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPP 754
Score = 27.9 bits (59), Expect = 2.4
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = -1
Query: 251 PPPPXXFLFXPPPPXPPXXXPPPPL 177
PPPP + P P P PP P+
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPI 757
Score = 27.9 bits (59), Expect = 2.4
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +2
Query: 383 PPGXGXGGXTPXPXTXPPPPPP 448
PP G G P P PPPPPP
Sbjct: 765 PPPPGVAGAGPPP---PPPPPP 783
Score = 27.5 bits (58), Expect = 3.2
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -1
Query: 251 PPPPXXFLFXPPPPXPPXXXPPPP 180
PPPP + PP PP PPPP
Sbjct: 763 PPPPPPGVAGAGPPPPP---PPPP 783
Score = 26.2 bits (55), Expect = 7.3
Identities = 13/31 (41%), Positives = 14/31 (45%), Gaps = 7/31 (22%)
Frame = -1
Query: 251 PPPPXXFLFXP-------PPPXPPXXXPPPP 180
PPPP + P PPP P PPPP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.0 bits (52), Expect(2) = 0.54
Identities = 11/34 (32%), Positives = 12/34 (35%)
Frame = +3
Query: 342 KAPXTPGPXXXXXXPPGXGGGXXLPRXKPXPPPP 443
+ P P P P G G P PPPP
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
Score = 23.4 bits (48), Expect(2) = 0.54
Identities = 9/24 (37%), Positives = 11/24 (45%)
Frame = +3
Query: 540 PXGXPPPPHXPXXTXPTXXRXPXP 611
P G PP P + P+ R P P
Sbjct: 355 PQGRSAPPPPPPRSAPSTGRQPPP 378
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.9 bits (64), Expect = 0.59
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = -2
Query: 454 RXGXGGGGXGXXRGSXXPPPXPGG 383
R GGG G GS PPP PGG
Sbjct: 183 RGHNGGGFGGFGGGSGGPPPGPGG 206
Score = 25.8 bits (54), Expect = 9.7
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = -3
Query: 447 GGGGGGXVXGXGVXPPXPXPGGXXXXXRGRG 355
GGG GG G G PP P G G G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEG 217
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 29.5 bits (63), Expect = 0.78
Identities = 14/28 (50%), Positives = 14/28 (50%)
Frame = +1
Query: 169 GKXRGGGGXXXGGXGGGGXKRNXXGGGG 252
G RGG G GG GG G R GGG
Sbjct: 12 GGSRGGRGGFNGGRGGFGGGRGGARGGG 39
Score = 26.6 bits (56), Expect = 5.5
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +1
Query: 178 RGGGGXXXGGXGGGGXKRNXXGGG 249
RGG G GG GG R GGG
Sbjct: 8 RGGRGGSRGGRGGFNGGRGGFGGG 31
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.4
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = -1
Query: 251 PPPPXXFLFXPPPPXPPXXXPPPP 180
PPPP F PP PP PPPP
Sbjct: 10 PPPPPPPGFEPPSQPPP---PPPP 30
Score = 27.9 bits (59), Expect = 2.4
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +3
Query: 540 PXGXPPPPHXPXXTXPTXXRXPXPP 614
P G PPPP P P P PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 26.6 bits (56), Expect = 5.5
Identities = 10/25 (40%), Positives = 11/25 (44%)
Frame = +3
Query: 540 PXGXPPPPHXPXXTXPTXXRXPXPP 614
P PPPP P P+ P PP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.8
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = -1
Query: 221 PPPPXPPXXXPPPPLFXP 168
P PP PP PPPP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPP 1722
Score = 27.5 bits (58), Expect = 3.2
Identities = 13/29 (44%), Positives = 13/29 (44%), Gaps = 5/29 (17%)
Frame = -1
Query: 248 PPPXXFLFXPPPPXP-----PXXXPPPPL 177
PPP PPP P P PPPPL
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPL 1735
Score = 26.6 bits (56), Expect = 5.5
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = -1
Query: 251 PPPPXXFLFXPPPPXPPXXXPPPPLFXP 168
PPPP + PPPP P PP P
Sbjct: 1707 PPPPP--MSVPPPPSAPPMPAGPPSAPP 1732
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 26.2 bits (55), Expect = 7.3
Identities = 15/33 (45%), Positives = 15/33 (45%), Gaps = 6/33 (18%)
Frame = +1
Query: 169 GKXRGGGGXXXGGXGG------GGXKRNXXGGG 249
G RGG G GG GG GG R GGG
Sbjct: 141 GGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGG 173
Score = 25.8 bits (54), Expect = 9.7
Identities = 14/29 (48%), Positives = 14/29 (48%), Gaps = 5/29 (17%)
Frame = +1
Query: 178 RGGGGXXXGGXGG-----GGXKRNXXGGG 249
RGG G GG GG GG R GGG
Sbjct: 137 RGGRGGFRGGRGGSRGGFGGNSRGGFGGG 165
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 22.2 bits (45), Expect(2) = 8.2
Identities = 11/36 (30%), Positives = 13/36 (36%), Gaps = 1/36 (2%)
Frame = +3
Query: 345 APXTPGPXXXXXXPPGXGGGXXLPRXK-PXPPPPXP 449
AP P P P G +P+ PP P P
Sbjct: 1101 APPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVP 1136
Score = 21.8 bits (44), Expect(2) = 8.2
Identities = 9/27 (33%), Positives = 9/27 (33%)
Frame = +3
Query: 546 GXPPPPHXPXXTXPTXXRXPXPPXPXP 626
G PP P P PP P P
Sbjct: 1177 GIPPVPKPAAGVPPVPPPSEAPPVPKP 1203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,430,599
Number of Sequences: 5004
Number of extensions: 17818
Number of successful extensions: 250
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 523262732
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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