Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_P09
(916 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 132 7e-32
SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 109 4e-25
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 43 5e-05
SPAC14C4.08 |mug5||meiotically upregulated gene Mug5|Schizosacch... 27 3.7
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom... 26 6.5
SPBC1289.02c |uap2||U2 snRNP-associated protein Uap2|Schizosacch... 26 8.6
SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr 1||... 26 8.6
SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase |Schizosacch... 26 8.6
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 8.6
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 132 bits (319), Expect = 7e-32
Identities = 63/133 (47%), Positives = 90/133 (67%)
Frame = +1
Query: 85 MAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEK 264
MA ++ G +A + +LR+Q++ ++S F L I+QVGGREDSNVY+RMK +AA +
Sbjct: 1 MALLLEGTSLARKVREELREQISSIKSVDPYFNVSLKIIQVGGREDSNVYVRMKTRAANE 60
Query: 265 IGIAAEHIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDK 444
GI+ EH+ P DITE +LL I NE P+VHGIIVQ+PL + I+ +T+AV+P+K
Sbjct: 61 AGISCEHVNFPEDITEYDLLLAIKGFNEDPTVHGIIVQLPLPAH--INEQIITEAVAPEK 118
Query: 445 DVDGLNTINEGRV 483
DVDG N G++
Sbjct: 119 DVDGFCETNLGKL 131
Score = 63.7 bits (148), Expect = 3e-11
Identities = 31/75 (41%), Positives = 45/75 (60%)
Frame = +2
Query: 518 TPAGCVELIKKXGVTIAGKNVVVLXPSRXVGXPVSXLLKWEHATVXVCHSKTXXLXEITX 697
TP G + ++K G+ + GK+ VV+ S VG P+S LL+ +ATV +CHSKT + +I
Sbjct: 144 TPKGIMCILKHYGINVQGKHAVVIGRSNIVGRPMSILLEKANATVTLCHSKTESIADIVR 203
Query: 698 TXXXLVVAIXPPXMV 742
T +V AI P V
Sbjct: 204 TADIVVAAIGIPHFV 218
>SPBC2G2.08 |ade9||C-1-
tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr
ogenase/methylenetetrahydrofolatecyclohydrolase/formylte
trahydrofolatesynthetase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 969
Score = 109 bits (263), Expect = 4e-25
Identities = 56/136 (41%), Positives = 85/136 (62%)
Frame = +1
Query: 88 AQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKI 267
A I++G +A ++ + ++V L++K F+P LAIVQVG REDSNVY+RMK KAA +
Sbjct: 33 ANILNGKLLARKLQRSVAEEVQALKAKDRNFKPALAIVQVGKREDSNVYVRMKEKAARLV 92
Query: 268 GIAAEHIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKD 447
GI ++ P I LL ++ LN+ +VHG++VQ+PL ++ VT++++P KD
Sbjct: 93 GIDFKYCPFPETIQMPALLHELKKLNDDHTVHGVLVQLPLPKH--LNERTVTESITPPKD 150
Query: 448 VDGLNTINEGRVAVGD 495
VDG N G +A D
Sbjct: 151 VDGFGAFNIGLLAKND 166
Score = 66.1 bits (154), Expect = 7e-12
Identities = 33/81 (40%), Positives = 47/81 (58%)
Frame = +2
Query: 500 PDSFLXTPAGCVELIKKXGVTIAGKNVVVLXPSRXVGXPVSXLLKWEHATVXVCHSKTXX 679
P + TP G +EL+K +++AG N VVL S VG P+S LL+ ++ATV VCHSKT
Sbjct: 169 PIHYPCTPKGIMELLKDNKISVAGLNAVVLGRSDIVGNPISYLLRKDNATVTVCHSKTKD 228
Query: 680 LXEITXTXXXLVVAIXPPXMV 742
L + ++ A+ P V
Sbjct: 229 LIQHISNADLVIAALGKPEFV 249
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 43.2 bits (97), Expect = 5e-05
Identities = 35/123 (28%), Positives = 55/123 (44%)
Frame = +1
Query: 91 QIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIG 270
+++ VA + L+Q V F P+L + +Y K +IG
Sbjct: 14 KVVYASRVAETFVEQLKQHVNLFE-----FAPKLVGFLSNSDPAARMYADWTNKTCTEIG 68
Query: 271 IAAEHIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDV 450
E +P+D +L I N PSV+GI++ P+ +D D + + VSPDKDV
Sbjct: 69 FQYELREVPKD----DLEDAIVEANNDPSVNGIMIYFPVFNDGQ-DQY-LQQVVSPDKDV 122
Query: 451 DGL 459
+GL
Sbjct: 123 EGL 125
>SPAC14C4.08 |mug5||meiotically upregulated gene
Mug5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 179
Score = 27.1 bits (57), Expect = 3.7
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
Frame = +1
Query: 172 SGFEPRLAIVQVGGRE-DSNVYIRMKLKAAEKI 267
SGFE R+ I++ E DSN+YIR+K + K+
Sbjct: 11 SGFENRIKILEDLMNEFDSNLYIRLKRECDFKL 43
>SPBC1A4.07c |||U3 snoRNP-associated protein
Sof1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 436
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/51 (25%), Positives = 24/51 (47%)
Frame = +1
Query: 136 LRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHI 288
L + +T+LR+ + P A V G ED N+Y+ + + +H+
Sbjct: 223 LTKLITKLRTNSISWNPMEAFNFVAGSEDHNLYMYDMRNLKRALHVYKDHV 273
>SPBC1289.02c |uap2||U2 snRNP-associated protein
Uap2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +1
Query: 310 EIELLAKITSLNESPSVHGIIVQMPLD-SDHAIDAHRVTDAVSPDKDVDGLNTI 468
+I +L I +L E +++ + D ++ A RVT+ V DK+ DG+ T+
Sbjct: 249 KIVVLKHIFTLEELDKTPELLIDLKDDITEEAEKCGRVTNVVLYDKEPDGVVTV 302
>SPAC6F12.03c |fsv1||SNARE Fsv1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 247
Score = 25.8 bits (54), Expect = 8.6
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +1
Query: 109 EVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRM--KLKAAEK 264
E+ S++ D+RQ++ +L + S E I + RE IRM +L++AE+
Sbjct: 31 EIESSLK-DVRQELQKLNEEQSRLEKNAQIPEYRVRESEAFLIRMQRRLESAEE 83
>SPBC16C6.01c ||SPBC543.11c|lysine methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 473
Score = 25.8 bits (54), Expect = 8.6
Identities = 16/34 (47%), Positives = 20/34 (58%)
Frame = +1
Query: 232 YIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKI 333
YI KL A G+A + I DI E+E+LAKI
Sbjct: 23 YISPKLYIASS-GVAGDGIFSTFDIDELEVLAKI 55
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 8.6
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Frame = -2
Query: 171 PF*TQPRHLLTQVIFYRSCNFNPRYNLCH--LSPENCLVGRSNPQILRIPY 25
PF + +L+Q +F +S F L H L PEN L+ + + +R+PY
Sbjct: 459 PFPLKHIQMLSQQLF-KSVAFLHSLGLVHTDLKPENVLLVSNASRTIRLPY 508
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,044,643
Number of Sequences: 5004
Number of extensions: 53527
Number of successful extensions: 122
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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