Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_O02
(877 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 265 5e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 260 2e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 133 4e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 116 4e-27
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.16
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 3.5
SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 26 6.1
SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase Exg2|Schizosacch... 26 6.1
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 265 bits (650), Expect = 5e-72
Identities = 125/229 (54%), Positives = 155/229 (67%)
Frame = +1
Query: 133 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 312
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 313 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 492
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 493 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIXPAASGFHC 672
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++ PA
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 673 RXRALHSIXXXXXXXXXXXXCFHGSTMKPSMTSAXGNLXLXRPTYXNLN 819
+S+ F + NL + RP+Y NLN
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDN-ESCYDICRRNLDIERPSYENLN 228
Score = 32.3 bits (70), Expect = 0.093
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +2
Query: 650 PRPQVSTAVXEPYTLSSPPTQPLSHSDCAFM 742
P PQVST+V EPY L +DC FM
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFM 203
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 260 bits (636), Expect = 2e-70
Identities = 127/234 (54%), Positives = 157/234 (67%), Gaps = 5/234 (2%)
Frame = +1
Query: 133 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 297
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 298 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 477
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 478 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIXPAA 657
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++ PA
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 658 SGFHCRXRALHSIXXXXXXXXXXXXCFHGSTMKPSMTSAXGNLXLXRPTYXNLN 819
+S+ F + NL + RPTY NLN
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDN-EACYDICRRNLDIERPTYENLN 232
Score = 35.1 bits (77), Expect = 0.013
Identities = 16/31 (51%), Positives = 18/31 (58%)
Frame = +2
Query: 650 PRPQVSTAVXEPYTLSSPPTQPLSHSDCAFM 742
P PQVST+V EPY L +SDC FM
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFM 207
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 133 bits (321), Expect = 4e-32
Identities = 65/174 (37%), Positives = 94/174 (54%)
Frame = +1
Query: 133 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 312
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 313 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 492
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 493 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIXPA 654
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PA
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 116 bits (279), Expect = 4e-27
Identities = 62/174 (35%), Positives = 97/174 (55%), Gaps = 2/174 (1%)
Frame = +1
Query: 136 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 315
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 316 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 489
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 490 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIXP 651
D I + AD L+GF + H L+ERL+ Y KK +++ P
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 31.5 bits (68), Expect = 0.16
Identities = 21/79 (26%), Positives = 36/79 (45%)
Frame = +2
Query: 497 SASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSXPRPQVSTAV 676
S++ +S P+ S S T S +P G+ S +P+T SS +T+
Sbjct: 377 SSTGTSSSPLLSTSTSCTTSTSIPPTGN-----STTPVTPTVPPTSSSTPLTTTNCTTST 431
Query: 677 XEPYTLSSPPTQPLSHSDC 733
PYT + + PL+ ++C
Sbjct: 432 SVPYTSTPVTSTPLATTNC 450
Score = 29.5 bits (63), Expect = 0.66
Identities = 22/81 (27%), Positives = 39/81 (48%), Gaps = 2/81 (2%)
Frame = +2
Query: 491 TESASSLTS--VPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSXPRPQV 664
T +SSL++ P + +S+ S+ +P + + +PL T + +S S P
Sbjct: 124 TGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSNPLPTTSTSCTTSTSIPPTGG 183
Query: 665 STAVXEPYTLSSPPTQPLSHS 727
S+++ P T + PPT S S
Sbjct: 184 SSSLSTPITPTVPPTSTSSTS 204
Score = 27.1 bits (57), Expect = 3.5
Identities = 24/71 (33%), Positives = 33/71 (46%)
Frame = +2
Query: 515 SVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSXPRPQVSTAVXEPYTL 694
S P+ +S SST S GS S SPL + + +S S P S+++ P T
Sbjct: 84 STPIITESTSSTSSASTT--GS-----SSSPLPSTSTSCTTSTSIPPTGGSSSLSTPITP 136
Query: 695 SSPPTQPLSHS 727
+ PPT S S
Sbjct: 137 TVPPTSTSSTS 147
Score = 25.8 bits (54), Expect = 8.1
Identities = 19/86 (22%), Positives = 41/86 (47%), Gaps = 5/86 (5%)
Frame = +2
Query: 491 TESASSLTSVPVCKDS*SSTPSVEVPALGSLPY*WSVSPLTTAR-SLNWSSPSXPRP--- 658
T + ++ TS+P +STP S ++ +P+T++ +++ S+P P
Sbjct: 470 TTNCTTSTSIPYTSTPVTSTPLTTTNCTTSTSVPYTSTPVTSSNYTISSSTPVTSTPVTT 529
Query: 659 -QVSTAVXEPYTLSSPPTQPLSHSDC 733
+T+ YT + + PL+ ++C
Sbjct: 530 TNCTTSTSVLYTSTPVTSTPLATTNC 555
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 27.1 bits (57), Expect = 3.5
Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +2
Query: 551 PSV-EVPALGSLPY*WSVSPLTTARSLNWSSPSXPRPQVSTAVXEPYTLSSPPTQPLS 721
PSV + PA+ +P SV P + PS P+P V+ V E ++ PP P++
Sbjct: 557 PSVPQRPAVPVVPEALSV-PQPPVAPVAPEVPSVPQPPVAPVVPEAPSVPQPPVAPVA 613
>SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase
Ppk30|Schizosaccharomyces pombe|chr 2|||Manual
Length = 953
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = +2
Query: 548 TPSVEVPALGSLPY*WSVSPLTTARSLNWSSPSXPRPQVSTAVXEPYTLSSPPTQPLSHS 727
TPS + L S P S+ P+ T ++ + + P RP + + + S P+S +
Sbjct: 356 TPSGSLTPLSSRPAHTSLPPIPTVQTTSSNVPPVNRPSLKSKSPSVSNILSNQLSPISSA 415
Query: 728 D 730
+
Sbjct: 416 N 416
>SPAC12B10.11 |exg2||glucan 1,3-beta-glucosidase
Exg2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 570
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -2
Query: 774 GRCHRWLHXGPMKAQSEWLKGCVGGED 694
G + W H G + Q EWL G V GE+
Sbjct: 280 GNQNSWNHGGYLN-QMEWLDGTVKGEE 305
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 212 STASSLMARCPQTRPSGVETILSTLSSARPELAST 316
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 231 WPDAHRQDHRGWRRFFQHFLQR 296
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,511,994
Number of Sequences: 5004
Number of extensions: 73431
Number of successful extensions: 221
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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