Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_N14
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 35 0.018
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 34 0.024
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.38
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.51
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.88
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.7
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.7
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.7
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 26 6.2
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.2
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.2
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 26 8.2
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 34.7 bits (76), Expect = 0.018
Identities = 16/52 (30%), Positives = 20/52 (38%)
Frame = +3
Query: 675 PXPDPSILXPXXXXXXSLPXPXXXXXXXXXPPPXXXPXPXXPPXPXPXPPPP 830
P P P+++ P +P P PPP P P P PPPP
Sbjct: 733 PPPPPAVIVPTPAPAP-IPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.3 bits (75), Expect = 0.024
Identities = 23/81 (28%), Positives = 29/81 (35%), Gaps = 1/81 (1%)
Frame = +3
Query: 591 PXSR-PXLPRLPXXXPXKVIHNIXXAXXRPXPDPSILXPXXXXXXSLPXPXXXXXXXXXP 767
P +R P +P+L P + + A P P PS P + P P P
Sbjct: 1007 PLARVPPVPKLSSKAPPVPLPS---ADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAP 1063
Query: 768 PPXXXPXPXXPPXPXPXPPPP 830
PP P P P P PP
Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPP 1084
Score = 30.3 bits (65), Expect = 0.38
Identities = 18/61 (29%), Positives = 19/61 (31%), Gaps = 2/61 (3%)
Frame = +3
Query: 675 PXPDPSILXPXXXXXXS--LPXPXXXXXXXXXPPPXXXPXPXXPPXPXPXPPPPXXRXXX 848
P P PS+ P S P P PPP P P P PPP
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 849 P 851
P
Sbjct: 1220 P 1220
Score = 28.3 bits (60), Expect = 1.5
Identities = 26/112 (23%), Positives = 34/112 (30%), Gaps = 7/112 (6%)
Frame = +3
Query: 516 PXAXXPEVSXEXVPPTCRRLMRNWXPXSRPXLPRLPXXXPXKVIHNIXXAXXRPXPDPSI 695
P P V+ VP + P P +P+ P + + P P PS+
Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPV----PSGAPPVPKPSV 1147
Query: 696 LXPXXXXXXS---LPXPXXXXXXXXXP----PPXXXPXPXXPPXPXPXPPPP 830
P +P P P PP P PP P P PP
Sbjct: 1148 AAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/47 (29%), Positives = 14/47 (29%)
Frame = +2
Query: 725 PSXSXXPXXXPXXXXPXXXPPXXXPXXPXPRPPSPXXPXXXPXPXXP 865
PS P P P PP P P P P P P P
Sbjct: 1174 PSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/54 (25%), Positives = 15/54 (27%)
Frame = +2
Query: 725 PSXSXXPXXXPXXXXPXXXPPXXXPXXPXPRPPSPXXPXXXPXPXXPXXXXXPP 886
PS + P P P P P P P P P P PP
Sbjct: 1107 PSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPP 1160
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.3 bits (65), Expect = 0.38
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +3
Query: 765 PPPXXXPXPXXPPXPXPXPPPP 830
PPP P PP P PPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.51
Identities = 27/92 (29%), Positives = 30/92 (32%)
Frame = +3
Query: 555 PPTCRRLMRNWXPXSRPXLPRLPXXXPXKVIHNIXXAXXRPXPDPSILXPXXXXXXSLPX 734
PPT + +R P S P P +P P P PS P SLP
Sbjct: 130 PPTPQSELR--PPTSAPPRPSIPPPSPASA-----------PPIPSKAPPIPS---SLPP 173
Query: 735 PXXXXXXXXXPPPXXXPXPXXPPXPXPXPPPP 830
P PP PP P PPPP
Sbjct: 174 PAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/55 (30%), Positives = 17/55 (30%), Gaps = 2/55 (3%)
Frame = +2
Query: 728 SXSXXPXXXPXXXXPXXX--PPXXXPXXPXPRPPSPXXPXXXPXPXXPXXXXXPP 886
S S P P P PP P P PPSP P P PP
Sbjct: 119 SASAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPP 173
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.88
Identities = 29/121 (23%), Positives = 35/121 (28%), Gaps = 5/121 (4%)
Frame = +3
Query: 483 LPXEAGGRRAEPXAXX---PEVSXEXVPPTCRRLMRNWXPXSRPXLP--RLPXXXPXKVI 647
+P GR A P P + P + R + N P P +P P P +
Sbjct: 351 IPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSN-PPAPPPAIPGRSAPALPP---L 406
Query: 648 HNIXXAXXRPXPDPSILXPXXXXXXSLPXPXXXXXXXXXPPPXXXPXPXXPPXPXPXPPP 827
N P P P L P P PP P PP P P
Sbjct: 407 GNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAA 466
Query: 828 P 830
P
Sbjct: 467 P 467
Score = 26.2 bits (55), Expect = 6.2
Identities = 25/105 (23%), Positives = 30/105 (28%)
Frame = +3
Query: 516 PXAXXPEVSXEXVPPTCRRLMRNWXPXSRPXLPRLPXXXPXKVIHNIXXAXXRPXPDPSI 695
P P S +PP + P P P LP P + + + P
Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTP--PSLPPSAPPSLPPSAPPSLPMGAPAAPP 448
Query: 696 LXPXXXXXXSLPXPXXXXXXXXXPPPXXXPXPXXPPXPXPXPPPP 830
L P LP PP P PP P P P P
Sbjct: 449 LPPSAPIAPPLPA------GMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/44 (29%), Positives = 13/44 (29%)
Frame = +2
Query: 755 PXXXXPXXXPPXXXPXXPXPRPPSPXXPXXXPXPXXPXXXXXPP 886
P P PP P P PPS P P PP
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPP 458
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/37 (32%), Positives = 12/37 (32%)
Frame = +2
Query: 755 PXXXXPXXXPPXXXPXXPXPRPPSPXXPXXXPXPXXP 865
P P PP P PP P P P P P
Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 181 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 300
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.7
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 175 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 264
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -3
Query: 433 GRALDVLPRLFQSLLGLAVRVSERXPGDSW 344
G + L RLF SL GL ++ PG+ W
Sbjct: 411 GTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.2
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +1
Query: 88 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 267
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 268 SKDA 279
SKDA
Sbjct: 230 SKDA 233
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.2
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 145 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 321
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 322 LQQL 333
Q+
Sbjct: 73 NNQI 76
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/41 (29%), Positives = 22/41 (53%)
Frame = -2
Query: 413 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 291
AST+ K PWP + L +P + + CS+ +E ++ +
Sbjct: 38 ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,347,188
Number of Sequences: 5004
Number of extensions: 38348
Number of successful extensions: 236
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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