BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_L08
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 35 0.013
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 32 0.12
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.38
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 28 1.5
SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyc... 27 2.7
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 2.7
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 26 8.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.1
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 35.1 bits (77), Expect = 0.013
Identities = 16/49 (32%), Positives = 16/49 (32%)
Frame = +2
Query: 662 PXXCPPXXXXXXXXXXPXXPXXRPPPXXXPXXPXXPPXPPXPPXXXPXP 808
P PP P PPP P P PP P PP P P
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 31.9 bits (69), Expect = 0.12
Identities = 15/43 (34%), Positives = 26/43 (60%)
Frame = -2
Query: 164 NGNIQQDKNFLFNSQNLCYSLRASALGRCVDWLASDKTNXNLK 36
N ++ K+FLF ++ SL S+LGRC LAS + + +++
Sbjct: 209 NSELEWFKSFLFKKSSIDNSLLHSSLGRCFSALASAQADKSIE 251
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.3 bits (65), Expect = 0.38
Identities = 17/57 (29%), Positives = 17/57 (29%), Gaps = 2/57 (3%)
Frame = +2
Query: 644 PXYRPP--PXXCPPXXXXXXXXXXPXXPXXRPPPXXXPXXPXXPPXPPXPPXXXPXP 808
P PP P PP P P P P PP PP P P P
Sbjct: 424 PPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Score = 27.9 bits (59), Expect = 2.0
Identities = 23/86 (26%), Positives = 23/86 (26%), Gaps = 3/86 (3%)
Frame = +2
Query: 575 AXTXPPXSGXRXXSXSPXDHCXXPXYRPP--PXXCPPXXXXXXXXXXPXXPXXRPPPXXX 748
A PP S P P PP P PP P P P
Sbjct: 400 APALPPLGNASRTSTPPVP---TPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIA 456
Query: 749 PXXPXX-PPXPPXPPXXXPXPXXXXP 823
P P P PP PP P P
Sbjct: 457 PPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 26.2 bits (55), Expect = 6.1
Identities = 19/73 (26%), Positives = 19/73 (26%), Gaps = 2/73 (2%)
Frame = +2
Query: 659 PPXXCPPXXXXXXXXXXPXXPXXRPP--PXXXPXXPXXPPXPPXPPXXXPXPXXXXPXXX 832
PP PP P P PP P P P PP P P P
Sbjct: 415 PPVPTPPSLPPSAP---PSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPP 471
Query: 833 XXXXPXPXXXXXP 871
P P P
Sbjct: 472 AAPAPPPAPAPAP 484
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 28.3 bits (60), Expect = 1.5
Identities = 30/122 (24%), Positives = 45/122 (36%), Gaps = 5/122 (4%)
Frame = +3
Query: 15 HLTTREFLKIXIRLVAR*PVYTPP*SGSAQRITQVL*IKKKVFILLNVP--ILTRMST-- 182
HL RL P TPP S S+ + +K P +++ ST
Sbjct: 238 HLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTTSNASRQPSPLVSSKSTDN 297
Query: 183 LLEVCGP-LSPPSTPPLMENKVELPLKKIAAKRARESSSPPAGLVTPQPSDSEGEDEFSK 359
++ P L P T E K P +R E+S+ P + TPQ ++ K
Sbjct: 298 IIRSHSPVLLTPQTLSTSETKHIRPNNSTPYQRRAETSTKPKAVATPQKVEAPSAPRLQK 357
Query: 360 RS 365
R+
Sbjct: 358 RA 359
>SPBC19C7.05 |||cell wall organization protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 150
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/56 (28%), Positives = 16/56 (28%), Gaps = 3/56 (5%)
Frame = +2
Query: 644 PXYRPPPXXCPPXXXXXXXXXXPXXPXX---RPPPXXXPXXPXXPPXPPXPPXXXP 802
P PPP P P P RPPP P P PP P
Sbjct: 75 PLTAPPPYTAEPEARYNSTIHNPMPPMSQAYRPPPTEPPTVPAYETSSEFPPPAYP 130
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/50 (28%), Positives = 14/50 (28%)
Frame = +2
Query: 674 PPXXXXXXXXXXPXXPXXRPPPXXXPXXPXXPPXPPXPPXXXPXPXXXXP 823
PP P PPP P PP PP P P P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/49 (28%), Positives = 14/49 (28%)
Frame = +2
Query: 656 PPPXXCPPXXXXXXXXXXPXXPXXRPPPXXXPXXPXXPPXPPXPPXXXP 802
PPP P P P PP P PP PP P
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 25.8 bits (54), Expect = 8.1
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +2
Query: 47 DSSCRSLASLHTSLERKRAANNTSFVN*KESFYLVECSHSNQNVHSARSLRTPLTA 214
D CR + +L LER+R + + S+Y S Q +HS +LR + A
Sbjct: 1045 DECCRYIVALSCELERRRLGEEDTKRALELSYYF--ASADLQPMHSIIALRLAINA 1098
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/24 (41%), Positives = 10/24 (41%)
Frame = +2
Query: 719 PXXRPPPXXXPXXPXXPPXPPXPP 790
P PPP P P PP PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,201,302
Number of Sequences: 5004
Number of extensions: 38044
Number of successful extensions: 185
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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