Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP19_F_K13
         (897 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF...   115   1e-26
SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F ...   110   2e-25
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    27   3.6  
SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   8.4  

>SPBC1709.18 |tif452|SPBC409.01|translation initiation factor eIF4E
           4F complex subunit |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 243

 Score =  115 bits (276), Expect = 1e-26
 Identities = 61/166 (36%), Positives = 91/166 (54%), Gaps = 4/166 (2%)
 Frame = +3

Query: 189 LQNQWSLWFYDND-RNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDYAVFKQG 365
           LQ++W+LWF     +   W + L E+ +F TVE+FW ++  I   S L    DY+ F +G
Sbjct: 66  LQHEWTLWFLKPPTQGLEWSDLLKEIISFKTVEEFWGIFKTISKASMLPAKSDYSYFLKG 125

Query: 366 IRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENXE-NSDEICGAVVNVR 542
           IRP WED  N  GG+W  + + K + ++LD  WL +VL  IGE  +    E+ G V N+R
Sbjct: 126 IRPEWEDPQNMNGGKW--AYQSKHKGSNLDELWLYMVLAAIGETLDPTGKEVTGVVCNMR 183

Query: 543 PKVDKIAXWTADAMKQHATIEIGKKLKEXLGIHXQ--IGFPVHRDT 674
               +IA WT +   +    +IG + KE LGI  +  I +  H D+
Sbjct: 184 KGFYRIAVWTRNCNDKDVLEKIGLRFKEVLGISDKETIEYSAHEDS 229


>SPAC16E8.15 |tif45|tif1|translation initiation factor eIF4E, 4F
           complex subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 218

 Score =  110 bits (265), Expect = 2e-25
 Identities = 58/172 (33%), Positives = 87/172 (50%), Gaps = 4/172 (2%)
 Frame = +3

Query: 171 FDQRTRLQNQWSLWFY-DNDRNKTWEENLIELTTFDTVEDFWRLYHHIKLPSELRQGHDY 347
           F+ +  L   W+LWF         W E    + TF++VE+FW ++++I   S L    DY
Sbjct: 34  FNLKHPLARPWTLWFLMPPTPGLEWNELQKNIITFNSVEEFWGIHNNINPASSLPIKSDY 93

Query: 348 AVFKQGIRPMWEDDANKMGGRWLISLEKKQRFTDLDRFWLDVVLLLIGENXE-NSDEICG 524
           + F++G+RP WED  NK GG+W     K +    LD  WL  VL  IGE  +    E+ G
Sbjct: 94  SFFREGVRPEWEDVHNKTGGKWAFQ-NKGRGGNALDEMWLTTVLAAIGETLDPTGQEVMG 152

Query: 525 AVVNVRPKVDKIAXWTADAMKQHATIEIGKKLKEXLGI--HXQIGFPVHRDT 674
            V+N+R    ++A WT     +   +EIG + K+ L +     I F  H D+
Sbjct: 153 VVINMRKGFYRLAVWTKSCNNREVLMEIGTRFKQVLNLPRSETIEFSAHEDS 204


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2685

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 13/35 (37%), Positives = 19/35 (54%)
 Frame = +3

Query: 168 IFDQRTRLQNQWSLWFYDNDRNKTWEENLIELTTF 272
           IF+  + L+   +  FY N +NK W  N  +L TF
Sbjct: 510 IFEIESSLRLSSNKVFYHNPKNKKWLINTSDLITF 544


>SPAC1805.10 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 527

 Score = 25.8 bits (54), Expect = 8.4
 Identities = 13/38 (34%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = +3

Query: 366 IRPMWEDDANKMGGRWLIS-LEKKQRFTDLDRFWLDVV 476
           +  +WE   N   G +L   +EKK  F  +DR+W  V+
Sbjct: 161 VAALWEQ-FNIFHGSYLFDEIEKKSSFEIIDRWWTSVL 197


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,991,827
Number of Sequences: 5004
Number of extensions: 54273
Number of successful extensions: 156
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)