Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP19_F_C04
(932 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 123 3e-29
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 123 3e-29
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 33 0.044
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.18
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.71
SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe... 29 0.71
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 1.6
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 2.9
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 5.0
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 8.7
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 8.7
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 123 bits (297), Expect = 3e-29
Identities = 62/105 (59%), Positives = 80/105 (76%), Gaps = 2/105 (1%)
Frame = +2
Query: 176 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 355
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 356 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLI 484
WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL ++
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVL 105
Score = 53.6 bits (123), Expect = 4e-08
Identities = 23/29 (79%), Positives = 25/29 (86%)
Frame = +3
Query: 486 LDKHLIPKASNPESKVFYLKMKGDYYRYL 572
L+KHLIP A+ ESKVFY KMKGDYYRYL
Sbjct: 105 LEKHLIPAATTGESKVFYYKMKGDYYRYL 133
Score = 33.1 bits (72), Expect = 0.058
Identities = 22/58 (37%), Positives = 29/58 (50%)
Frame = +1
Query: 571 LAEVATGXTRHSVVEDSXKAYQDAFEISKAKMQPHTPP*GWVWR*IFSVXYYEILKFP 744
LAE G + S +AY+ A +I+ A++ P T P FSV YYEIL P
Sbjct: 133 LAEFTVGEVCKEAADSSLEAYKAASDIAVAELPP-TDPMRLGLALNFSVFYYEILDSP 189
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 123 bits (297), Expect = 3e-29
Identities = 82/196 (41%), Positives = 113/196 (57%), Gaps = 5/196 (2%)
Frame = +2
Query: 179 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 358
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 359 RVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLIT*Q-TPNS*S**SRK* 529
R++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L ++ PN+ S S+
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 530 SVLPQNE--RRLLQVPWQKWPQEXPDILL*RIXXKXTKMLLKSARRKCSPTHPHKAGSGV 703
+ + R L + + Q D L + K + A + +PTHP + G +
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSL-----EGYKAASEIATAELAPTHPIRLGLAL 177
Query: 704 KFSQSFIMRY*NSPDQ 751
FS F NSPD+
Sbjct: 178 NFS-VFYYEILNSPDR 192
Score = 56.4 bits (130), Expect = 5e-09
Identities = 44/134 (32%), Positives = 54/134 (40%)
Frame = +3
Query: 486 LDKHLIPKASNPESKVFYLKMKGDYYRYLGRSGHRRXQTFCCRGFXESXPRCF*NQQGEN 665
L+KHLIP A++ ESKVFY KMKGDYYRYL + E E
Sbjct: 106 LEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAEL 165
Query: 666 AAPHTPIRXXXXXXXXXXXXXDIKIPQTKACHLXQPGLPTMXXXELGPXERXFVQTIPPL 845
A H PIR + P +AC+L + E + + L
Sbjct: 166 APTH-PIRLGLALNFSVFYYEILNSPD-RACYLAKQAFDEAISELDSLSEESYKDS--TL 221
Query: 846 IMXLLXKYXXLWTS 887
IM LL LWTS
Sbjct: 222 IMQLLRDNLTLWTS 235
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 33.5 bits (73), Expect = 0.044
Identities = 25/104 (24%), Positives = 44/104 (42%)
Frame = +2
Query: 152 ISSLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 331
I S+ S S EEL+ R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2998 IMSITMSDSSAYGEELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 332 VVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 463
+R+ + +E+ + EY +E+ L++ C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.18
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +2
Query: 179 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 349
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 350 SSWRVIS 370
S+W S
Sbjct: 62 STWATFS 68
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.71
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 3/93 (3%)
Frame = +2
Query: 185 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 364
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 365 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 454
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 719
Score = 29.5 bits (63), Expect = 0.71
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +3
Query: 27 EFLKILXPSKPVYPFSAREKLILCLNLL 110
E +++L + ++PF EK++LCL LL
Sbjct: 81 ELIELLQKNHTIFPFELCEKIVLCLVLL 108
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
Frame = +3
Query: 69 FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 233
FS +E ILC L+L+V + ++ S+ + F +PR K N WPN+ S
Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98
Query: 234 DM 239
+
Sbjct: 99 HL 100
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 27.5 bits (58), Expect = 2.9
Identities = 18/58 (31%), Positives = 25/58 (43%)
Frame = -2
Query: 208 TLHQFFLVDGHRGRWKRTNSLMPLSEGEKCI*TSKFKHNISFSLALNGYTGFDGXKIL 35
TL FL DG RWK + S+ K +++ +LNGY D K+L
Sbjct: 52 TLKDEFLADGLESRWKTNGEKSSSIRYPLVMEFSQLKEDLTNRASLNGY---DSLKLL 106
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 5.0
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 326 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 442
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 8.7
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +2
Query: 314 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 448
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 8.7
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +3
Query: 81 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 218
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,337,188
Number of Sequences: 5004
Number of extensions: 62188
Number of successful extensions: 183
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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