Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP18_F_P16
         (886 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...   160   2e-40
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...   160   3e-40
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.67 
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi...    28   2.0  
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    26   6.2  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score =  160 bits (389), Expect = 2e-40
 Identities = 73/102 (71%), Positives = 88/102 (86%)
 Frame = +1

Query: 109 AKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIEYY 288
           +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRKSE+EYY
Sbjct: 14  SKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRKSELEYY 73

Query: 289 ALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 414
           A+L++  VHHYSGNNI+LGTACGK +RV  LA+ D GDSDI+
Sbjct: 74  AMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDSDIL 115


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score =  160 bits (388), Expect = 3e-40
 Identities = 73/104 (70%), Positives = 87/104 (83%)
 Frame = +1

Query: 103 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 282
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63

Query: 283 YYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDSDII 414
           YYA+L+K  VHHY+G NI+LGTACGK +RV  LAITD GDSDI+
Sbjct: 64  YYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDSDIL 107


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.67
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +1

Query: 115 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 240
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPAC977.14c |||aldo/keto reductase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 351

 Score = 27.9 bits (59), Expect = 2.0
 Identities = 15/45 (33%), Positives = 26/45 (57%)
 Frame = +1

Query: 223 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 357
           ++ +VI++K   P+RK  I+ +  L+  GVH    ++ EL   CG
Sbjct: 87  RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
 Frame = +1

Query: 94  SKMVAAKK-QKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 264
           +K +A KK  KK ++++        K      G K+ +K +R+G+  LVI+A +  P+
Sbjct: 29  AKPLAPKKLNKKMMKTVKK----ASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPM 82


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,503,791
Number of Sequences: 5004
Number of extensions: 39734
Number of successful extensions: 74
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 74
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 74
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)