BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_M02
(858 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 59 9e-10
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 46 5e-06
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.5
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 3.4
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 3.4
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 6.0
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 6.0
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 26 6.0
SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid transporter|Schizo... 26 7.9
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 58.8 bits (136), Expect = 9e-10
Identities = 39/124 (31%), Positives = 66/124 (53%), Gaps = 4/124 (3%)
Frame = +1
Query: 208 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 375
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 376 ASWSDSELLPASCAWVFPYLGIMQFNKXNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 555
S+++SEL A + P + +++ + A++ + D L ++T+LV R+T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 556 LADV 567
LAD+
Sbjct: 157 LADI 160
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 46.4 bits (105), Expect = 5e-06
Identities = 41/168 (24%), Positives = 74/168 (44%), Gaps = 8/168 (4%)
Frame = +1
Query: 88 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 267
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 268 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 423
ADG L + Y+ E L + +A + +W + + +++ P + WV
Sbjct: 58 ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117
Query: 424 FPYLGIMQFNKXNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADV 567
+ G + + + + + + + +LK+ + + RT+LV +R TLAD+
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADL 165
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +2
Query: 482 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 574
TY PP Y D+FS APS+ + S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 3.4
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +1
Query: 115 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 294
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 295 SN 300
SN
Sbjct: 612 SN 613
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +2
Query: 344 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 439
+W+P PV SG T C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 6.0
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -2
Query: 284 STFPSALSNAGTFPAGNFFKKSSDL 210
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 6.0
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +1
Query: 295 SNAIAYYVANESLRGGDLATQARVWQWAS 381
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 26.2 bits (55), Expect = 6.0
Identities = 22/66 (33%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +1
Query: 13 YRSHLYGEFLKILPRPPLLFPKTPTMAAGVLYTYPENFRAYKALIAAQYSG-TDVKVAPN 189
++ + YG+ L + L+ + A G +YT E+FRA K+L S T V+
Sbjct: 304 FKFNYYGQDA-YLTQSSQLYLEAALPALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELP 362
Query: 190 FV-FGE 204
FV FGE
Sbjct: 363 FVNFGE 368
>SPBC1652.02 |aap1|SPBC16A3.20c|APC amino acid
transporter|Schizosaccharomyces pombe|chr 2|||Manual
Length = 594
Score = 25.8 bits (54), Expect = 7.9
Identities = 19/60 (31%), Positives = 27/60 (45%)
Frame = +1
Query: 52 PRPPLLFPKTPTMAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLK 231
P P ++ P T T G + +Y E L + K+ P+ F E + SEDFLK
Sbjct: 16 PVPEIIVPST-TNGNGTIESYKEKSVGTSFLDFFR----SYKLRPDNEFAEVHNSEDFLK 70
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,906,583
Number of Sequences: 5004
Number of extensions: 59940
Number of successful extensions: 187
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -