Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP18_F_L21
(854 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 121 2e-28
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 118 1e-27
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.16
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 29 0.64
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 1.5
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 7.8
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 121 bits (291), Expect = 2e-28
Identities = 61/106 (57%), Positives = 80/106 (75%), Gaps = 2/106 (1%)
Frame = +1
Query: 199 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 378
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 379 WRVISSIEQK--TXGSERKQQMAKEYRVKVXKELXEICYDVLGLLD 510
WR+ISSIEQK + G+ R+ + KEYR K+ EL +IC+DVL +L+
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLE 106
Score = 48.4 bits (110), Expect = 1e-06
Identities = 33/90 (36%), Positives = 40/90 (44%)
Frame = +3
Query: 510 QHLIPKASXPXSKVFYLXMXGDYYRYLGXSGHXXNXTFCCXGFXKAXQXAFEIXKAXMXP 689
+HLIP A+ SKVFY M GDYYRYL +A + A +I A + P
Sbjct: 107 KHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPP 166
Query: 690 XXPHXAGSXX*ISXXXXYXILNSPXXAXPL 779
P G S Y IL+SP A L
Sbjct: 167 TDPMRLGLALNFS-VFYYEILDSPESACHL 195
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 118 bits (283), Expect = 1e-27
Identities = 58/105 (55%), Positives = 77/105 (73%), Gaps = 2/105 (1%)
Frame = +1
Query: 202 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 381
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 382 RVISSIEQK--TXGSERKQQMAKEYRVKVXKELXEICYDVLGLLD 510
R++SSIEQK + G+ + ++ KEYR K+ +EL IC D+L +L+
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLE 107
Score = 52.4 bits (120), Expect = 8e-08
Identities = 35/94 (37%), Positives = 40/94 (42%)
Frame = +3
Query: 510 QHLIPKASXPXSKVFYLXMXGDYYRYLGXSGHXXNXTFCCXGFXKAXQXAFEIXKAXMXP 689
+HLIP A+ SKVFY M GDYYRYL + + A EI A + P
Sbjct: 108 KHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAP 167
Query: 690 XXPHXAGSXX*ISXXXXYXILNSPXXAXPLXXXA 791
P G S Y ILNSP A L A
Sbjct: 168 THPIRLGLALNFS-VFYYEILNSPDRACYLAKQA 200
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.16
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 3/67 (4%)
Frame = +1
Query: 202 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 372
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 373 SSWRVIS 393
S+W S
Sbjct: 62 STWATFS 68
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 29.5 bits (63), Expect = 0.64
Identities = 24/104 (23%), Positives = 42/104 (40%)
Frame = +1
Query: 175 ISSLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKN 354
I S+ S S EEL+ R + + Y+ MA + E E ++ + LLS Y N
Sbjct: 2998 IMSITMSDSSAYGEELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056
Query: 355 VVGARRSSWRVISSIEQKTXGSERKQQMAKEYRVKVXKELXEIC 486
+R+ + +E+ + EY + + L + C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
Frame = +2
Query: 92 FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 256
FS +E ILC L+L+V + ++ S+ + F +PR K N WPN+ S
Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98
Query: 257 DM 262
+
Sbjct: 99 HL 100
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 7.8
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +2
Query: 104 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 241
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,398,399
Number of Sequences: 5004
Number of extensions: 41357
Number of successful extensions: 109
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 424464280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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